Relaxin-3 (RLX3) is recently discovered peptide of the insulin superfamily. The highly-conserved relaxin family peptide receptor 3(RXFP3) signalling system is associated with stress response and feeding behaviour. Central RLX3 injections stimulate feeding via RXFP3 in the paraventricular nucleus (PVN). We hypothesize that RLX3 exerts its orexigenic effect through inhibition of PVN oxytocin neurons activity. To investigate the influence of selective RXFP3 agonist RXFP#-A2, on the activity of magnocellular PVN neurons whole cell patch clamp recordings were performed on the rat brain slices. We have shown that selective RXFP3 agonist, reversibly inhibits electrical activity of magnocellular PVN neurons. Characterization of the recorded neurons was based on their electrophysiological properties and identification of their neurochemical content. Responsiveness of RLX3 neurons to stress factors and their impact on feeding behaviour allow us to hypothesize that this peptide is a bridge between chronic stress and overeating. Future patch clamp experiments with RLX3 antagonist, tract-tracing and behavioural studies will allow to further characterize the role of RLX3 in stress inducted overeating.