Investigation of possible ecotoxic effects of acrylamide on liver with the azaserine-rat model
Treść / Zawartość
In this study, when acrylamide is taken into the body by nutrition, it was aimed to investigate its eco- toxic and/or carcinogenic effects on the liver. The Azaserine-Rat model developed by Longnecker and Curphey was used in this research, and in total 60 Wistar Albino race male rats were used, including 10 rats in each group. The rats were fed for 16 weeks by adding acrylamide at rates of 5 mg/kg/day and 10 mg/kg/day in drinking water. Moreover, neoplastic structures were formed by azaserine application and its effect on the development of these neoplastic structures was also investigated. As a result of this study, it was determined that ecotoxic and histopathological alterations, together with atypical cells, focuses formed in the livers of the rats in the group to which azaserine was applied and in the livers of the rats in the groups that included 5 and 10 mg/kg/day acrylamide in their drinking water. Moreover, it was found that the development (average focus diameter and focus volume) of neoplastic structures formed with azaserine was increased by 5 and 10 mg/kg/day acrylamide. These results make it possible for the probability of acrylamide to be a cancer initiator in the livers of rats.
- 1. IARC (International Agency for Research on Cancer). Acrylamide, IARC monographs on the evaluation of carcinogenic risk to humans, some industrial chemicals. Lyon, Fransa. Internat. Agency for Res. on Cancer 60, 389,1994.
- 2. European Union Risk Assessment. Report acrylamide. European Union. Luxemburg, pp. 210, 2002.
- 3. MANIÈRE I., GODARD T., DOERGE D.R., CHURCHWEEL M.I., GUFFROY M., LAURENTIE M., POUL J.M. DNA damage and DNA adduct formation in rat tissues following oral administration of acrylamide. Mut. Res. 580, 119, 2005.
- 4. MOTTRAM D.S., WEDZICHA B.L., DODSON A.T. Acrylamide is formed in the maillard reaction. Nature 419,448, 2002.
- 5. STADLER R.H., BLANK I., VORGA N., ROBERT F., HAV J., GUY P.A., ROBERT M.C., REIDIKER S. Acrylamide from Maillard reaction products. Nature 419,449, 2002.
- 6. YENER Y., KALIPCI E. The carcinogenic effects of acrylamide formed during cooking of some foods. Acad. J. of Cancer Res. 2,(1), 25, 2009.
- 7. BOYLE P., LEVIN B. WHO World cancer report. International Agency for Research on Cancer. Lyon. Fransa. 1-105, 2008.
- 8. YILDIZ H., UCMAN S., UNALDI N., OZTAS H. Potential neoplastic effects of parathion-methyl on rat liver. J. of Environ. Sei. 21, (5), 696, 2009.
- 9. YENER Y., DIKMENLI M. Increased micronucleus frequency in rat bone marrow after acrylamide treatment. Food and Chemical Toxicology 47, (8), 2120, 2009.
- 10. KALIPCI E., OZDEMIR C., Investigation of ecotoxic effect of 2,4-Dichlorophenoxyacetic acid dimethylamine on liver and pancreas. Asian J. of Chem. 24, (4), 1559, 2012.
- 11. KALIPCI E., OZDEMIR C. Investigation of the ecotoxicologic effect of pesticide industry wastewater on the pancreas and liver of rats. African J. of Biotec. 10, (12), 2290, 2011.
- 12. YENER Y., DIKMENLI M. The effects of acrylamide on the frequency of megakaryocyte emperipolesis and the mitotic activity of rat bone marrow cells. Journal of the Science of Food and Agriculture 91, (10), 1810, 2011.
- 13. LONGNECKER D.S., CURPHEY T.J. Adenocarcinoma of the pancreas in azaserine-rat-treated rats. Cancer Res. 35, 2249,1975.
- 14. OZTAS H., KOC A., YILDIZ D. Ultrastructural changes in pancreatic acinar cells of fatty acids fed rats. Indian Vet. J. 85, 67, 2008.
- 15. YILDIZ H., KOC A., OZTAS H., YILDIZ D. Inhibitory effects of aspirin on azaserine initiated pancreatic carcinogenesis in rat. Indian. Vet. J. 85, 187, 2008.
- 16. TYL R.W., FRIEDMAN M.A., LOSCO P.E., FISHER L.C., JOHNSON K.A., STROTHER D.E., WOLF C.H. Rat twogeneration reproduction and dominant lethal study of acrylamide in drinking water. Reprod. Toxicol. 14, 385, 2000.
- 17. GAREY J., FERGUSON S.A., PAULE M.G. Developmental and behavioral effects of acrylamide in fischer 344 rats. Neurotoxicol. and Teratol. 27, (4), 553, 2005.
- 18. WISE L.D., GORDON L.R., SOPERK.A., DUCHAID.M., MORRISSEY R.E. Developmental neurotoxicity evaluation of acrylamide in Sprague-Dawley rats. Neurotoxicol. and Teratol. 17, 189,1995.
- 19. RICE J.M. The carcinogenicity of acrylamide. Mut. Res. 580, 3, 2005.
- 20. FRIEDMAN M. A., DULAK L.H., STEDHAM M.A. A lifetime oncogenicity study in rats with acrylamide. Fund, and App. Toxicol. 27,(1), 95,1995.
- 21. LAFFERTY J.S., KAMENDULIS L.M., KASTER J., JIANG J., KLAUNING J.E. Subchronic acrylamide treatment induces a tissue-spesifik increase in DNA synthesis in the rat. Toxicol. Letters. 154, 95, 2004.
- 22. HAASE N.U., MATTHÄUS B., VOSMANN K. Approaches to minimize acrylamide formation in plant shown in the example of foods, from potato chips. Deutche Lebens Runds 99, 87, 2003 [In German],
- 23. PEDRESCHI F., KAACK K., GRANBY K. Reduction of acrylamide formation in fried potato slices. Lebensmitte- Wissen Und Tech. 37, 679, 2004.
- 24. TAREKE E., RYDBERG P., KARLSSON P. Analysis of acrylamide, a carcinogen formed in heated foodstuffs. J. of Agricul. and Food. Chem. 50, 4998, 2002.
- 25. TATATSUKI S., NEMOTO S., SASAKI K, MAITANI T. Production of acrylamide in agricultural products by cooking. J. of the Food Hyg. Soc. of Japan 45, (1), 44, 2004.
- 26. ZHANG Y., HAORAN F., ZHANG Y. Study on formation of acrylamide in asparagine-sugar microwave heating systems using UPLC-MS/MS analytical method. Food Chem. 108, (2), 542, 2007.