Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2012 | 34 | 3 |
Tytuł artykułu

Identification of desiccation-regulated genes by cDNA-AFLP in Haberlea rhodopensis: a resurrection plant

Treść / Zawartość
Warianty tytułu
Języki publikacji
The Balkan endemic plant species Haberlea rhodopensis belongs to the group of resurrection plants. The members of this small group of angiosperms tolerate extreme dehydration of their vegetative tissues, which are able to recover very rapidly upon rehydration. In this respect, resurrection plants are a unique model intensively studied to reveal the secrets of desiccation tolerance. To date, the knowledge on the molecular biology of H. rhodopensis is very limited. Here, we report on the application of a cDNAAFLP analysis to examine gene expression in leaves of Haberlea during dehydration. Twenty transcripts among 33 sequenced cDNA fragments appear to be involved in energy metabolism, transport, cell-wall biogenesis, signal transduction, or are probably transcription regulators according to their putative function. Expression patterns of two up-regulated (HrhDR8, HrhDR35) and two down-regulated (HrhDR6, HrhDR25) transcripts were verified by sqRTPCR analysis at different stages of water stress. The results demonstrated that two up-regulated transcripts HrhDR8 and HrhDR35 encoding putative succinate-dehydrogenase and xyloglucan endotransglucosylase/hydrolase (XTH), respectively, were induced during early stage of dehydration, persist in desiccated state, and subsequent rehydration of Haberlea. Their possible involvement in drought tolerance is discussed.
Słowa kluczowe
Opis fizyczny
  • AgroBioInstitute, 8 Dragan Tsankov Blvd, 1164 Sofia, Bulgaria
  • School of Forest Resources and Environmental Science, Michigan Technological University, 1400 Townsend Drive, Houghton, MI 49931-1295, USA
  • AgroBioInstitute, 8 Dragan Tsankov Blvd, 1164 Sofia, Bulgaria
  • AgroBioInstitute, 8 Dragan Tsankov Blvd, 1164 Sofia, Bulgaria
  • Bachem CW, van der Hoeven RS, de Bruijn SM, Vreugdenhil D, Zabeau M, Visser RG (1996) Visualization of differential gene expression using a novel method of RNA fingerprinting based on AFLP: analysis of gene expression during potato tuber development. Plant J 9:745–753
  • Bockel C, Salamini F, Bartels D (1998) Isolation and characterization of genes expressed during early events of the dehydration process in the resurrection plant Craterostigma plantagineum. J Plant Physiol 152:158–166
  • Bove J, Lucas P, Godin B, Oge L, Jullien M, Grappin P (2005) Gene expression analysis by cDNA-AFLP highlights a set of new signaling networks and translational control during seed dormancy breaking in Nicotiana plumbaginifolia. Plant Mol Biol 57:593–612
  • Cho SK, Kim JE, Park J-A, Eom TJ, Kim WT (2006) Constitutive expression of abiotic stress-inducible hot papper CaXTH3, which encodes a xyloglucan endotransglucosylase/hydrolase homolog, improves drought and salt tolerance in transgenic Arabidopsis plants. FEBS Lett 580:3136–3144
  • Collett H, Shen A, Gardner M, Farrant JM, Denby K, Illing N (2004) Towards transcripts profiling of desiccation tolerance in Xerophyta humilis: construction of a normalized 11 k X. humilis cDNA set and microarray expression analysis of 424 cDNAs in response to dehydration. Physiol Plant 122:39–53
  • Creste S, Neto AT, Figueira A (2001) Detection of single sequence repeat polymorphisms in denaturing polyacrylamide sequencing gels by silver staining. Plant Mol Biol Rep 19:299–306
  • de Diego JG, Rodríguez FD, Rodríguez Lorenzo JL, Grappin P, Cervantes E (2006) cDNA-AFLP analysis of seed germination in Arabidopsis thaliana identifies transposons and new genomic sequences. J Plant Physiol 63:452–462
  • Deng X, Phillips J, Mejier AH, Salamini F, Bartels D (2002) Characterization of five novel dehydration-responsive homeodomain leucine zipper genes from the resurrection plant Craterostigma plantagineum. Plant Mol Biol 49:601–610
  • Deng X, Phillips J, Bräutigam A, Engström P, Johannesson H, Ouwerkerk PB, Ruberti I, Salinas J, Vera P, Iannacone R, Meijer AH, Bartels D (2006) A homeodomain leucine zipper gene from Craterostigma plantagineum regulates abscisic acid responsive gene expression and physiological responses. Plant Mol Biol 61:469–489
  • Ditt RF, Nester EW, Comai L (2001) Plant gene expression response to Agrobacterium tumefaciens. Proc Natl Acad Sci USA 98:10954–10959
  • Ditzer A, Bartels D (2006) Identification of stress-responsive promoter elements and isolation of corresponding DNA binding proteins for the LEA gene CpC2 promoter. Plant Mol Biol 61:643–663
  • Djilianov D, Genova G, Parvanova D, Zapryanova N, Konstantinova T, Atanasov A (2005) In vitro culture of the resurrection plant Haberlea rhodopensis. Plant Cell Tiss Org Cult 80:115–118
  • Djilianov D, Ivanov S, Moyankova D, Miteva L, Kirova E, Alexieva V, Joudi M, Peshev D, Van den Ende W (2011) Sugar ratios, glutathione redox status and phenols in the resurrection species Haberlea rhodopensis and the closely related non-resurrection species Chirita eberhardtii. Plant Biol 13:767–776
  • Dubos C, Plomion C (2003) Identification of water-deficit responsive genes in maritime pine (Pinus pinaster Ait.) roots. Plant Mol Biol 51:249–262
  • Elorza A, Roschzttardtz H, Gómez I, Mouras A, Holuigue L, Araya A, Jordana X (2006) A nuclear gene for the iron-sulfur subunit of mitochondrial complex II is specifically expressed during Arabidopsis seed development and germination. Plant Cell Physiol 47:14–21
  • Furini A, Koncz C, Salamini F, Bartels D (1997) High level transcription of a member of a repeated gene family confers dehydration tolerance to callus tissue of Craterostigma plantagineum. EMBO J 16:3599–3608
  • Ganchev I (1950) Anabiotic desiccation resistance and other biological traits of Haberlea rhodopensis Friv Report of Institute of Botany. Bulg Acad Sci 1:191–214
  • Georgieva K, Maslenkova L, Peeva V, Markovska Yu, Stefanov D, Tuba Z (2005) Comparative study on the changes in photosynthetic activity of the homoiochlorophyllous desiccation-tolerant Haberlea rhodopensis and spinach leaves during desiccation and rehydration. Photosynth Res 85:191–203
  • Georgieva K, Szigeti Z, Sarvari E, Gaspar L, Maslenkova L, Peeva V, Peli E, Tuba Z (2007) Photosynthetic activity of homoiochlorophyllous desiccation tolerant plant Haberlea rhodopensis during dehydration and rehydration. Planta 225:955–964
  • Hilbricht T, Salamini F, Bartels D (2002) CpR18, a novel SAPdomain plant transcription factor, binds to a promoter region necessary for ABA mediated expression of the CDeT27–45 gene from the resurrection plant Craterostigma plantagineum Hochst. Plant J 31:293–303
  • Iturriaga G, Leyns L, Villegas A, Gharaibeh R, Salamini F, Bartels D (1996) A family of novel myb-related genes from the resurrection plant Craterostigma plantagineum. Plant Mol Biol 32:707–716
  • Iturriaga G, Gushman MA, Cushman JC (2006) An EST catalogue from the resurrection plant Selaginella lepidophylla reveals abiotic stress-adaptive genes. Plant Sci 170:1173–1184
  • Kubacka-Zebalska M, Kacperska A (1999) Low temperature induced modifications of cell wall content and polysaccharide composition in leaves of winter oilseed rape (Brassica napus L. var. oleifera L.). Plant Sci 148:59–67
  • Le T-N, McQueen-Mason SJ (2006) Desiccation-tolerant plants in dry environments. Rev Environ Sci Biotechnol 5:269–279
  • Le NT, Blomstedt CK, Kuang J, Tenlen J, Gaff DF, Hamill JD, Neale AD (2007) Desiccation-tolerance specific gene expression in the leaf tissue of the resurrection plant Sporobolus stapfianus. Funct Plant Biol 34:589–600
  • Liu Y-B, Lu S-M, Zhang J-F, Liu S, Lu Y-T (2007) A xyloglucan endotransglucosylase/hydrolase involves in growth of primary root and alters the deposition of cellulose in Arabidopsis. Planta 226:1547–1560
  • Mao C, Yi K, Yang L, Zheng B, Wu Y, Liu F, Wu P (2004) Identification of aluminium-regulated genes by cDNA-AFLP in rice (Oryza sativa L.): alumunium-regulated genes for the metabolism of cell wall components. J Exp Bot 55:137–143
  • Markovska YK, Tsonev TD, Kimenov GP, Tutekova AA (1994) Physiological changes in higher poikilohydric plants—Haberlea rhodopensis Friv. and Ramonda serbica Panc. during drought and rewatering at different light regimes. J Plant Physiol 144: 100–108
  • Markovska YK, Tutekova AA, Kimenov GP (1995) Ultrastructure of chloroplasts of poikilohydric plants Haberlea rhodopensis Friv. and Ramonda serbica Panc. during recovery from desiccation. Photosynthetica 31:613–620
  • Müller J, Sprenger N, Bortlik K, Boller T, Wiemken A (1997) Desiccation increases sucrose levels in Rhamonda and Haberlea, two genera of resurrection plants in the Gesneriaceae. Physiol Plant 100:153–158
  • Oliver MJ, Velten J, Mishler B (2005) Desiccation tolerance in bryophytes: a reflection of the primitive strategy for plant survival in dehydrating habitats? Integr Comp Biol 45:788–799
  • Peeva V, Maslenkova L (2004) Thermoluminescence study of photosystem II activity in Haberlea rhodopensis and spinach leaves during desiccation. Plant Biol 6:1–6
  • Ramanjulu S, Bartels D (2002) Drought- and desiccation induced modulation of gene expression in plants. Plant Cell Environ 25:141–151
  • Rascio N, La Rocca N (2005) Resurrection plants: the puzzle of surviving extreme vegetative desiccation. Crit Rev Plant Sci 24:209–225
  • Rodriguez MC, Edsgärd D, Hussain SS, Alquezar D, Rasmussen M, Gilbert T, Nielsen BH, Bartels D, Mundy J (2010) Transcriptomes of the desiccation-tolerant resurrection plant Craterostigma plantagineum. Plant J 63:212–228
  • Stefanov K, Markovska YK, Kimenov G, Popov S (1992) Lipid and sterol changes in leaves of Haberlea rhodopensis and Ramonda serbica at transition from biosis to anabiosis and vice versa caused by water stress. Phytochemistry 31:2309–2314
  • Toldi O, Tuba Z, Scott P (2009) Vegetative desiccation tolerance: is it a goldmine for bioengineering crops? Plant Sci 176:187–199
  • van Raemdonck D, Pesquet E, Cloquet S, Beeckman H, Boerjan W, Goffner D, Jaziri ME, Baucher M (2005) Molecular changes associated with the setting up of secondary growth in aspen. J Exp Bot 56:2211–2227
  • Vicré M, Sherwin HW, Driouich A, Jaffer MA, Farrant JM (1999) Cell wall characteristics and structure of hydrated and dry leaves of the resurrection plant Craterostigma wilmsii, a microscopical study. J Plant Physiol 155:719–726
  • Vicré M, Lerouxel O, Farrant J, Lerouge P, Driouich A (2004) Composition and desiccation-induced alterations in the cell wall of the resurrection plant Craterostigma wilmsii. Physiol Plant 120:229–239
  • Vos P, Hogers R, Bleeker M, Reijans M, Lee T, Hornes M, Frijters A, Pot J, Peleman J, Kuiper M, Zabeau M (1995) AFLP: a new technique for DNA fingerprinting. Nucl Acids Res 23:4407–4414
  • Yahubyan G, Gozmanova M, Denev I, Toneva V, Minkov I (2009) Prompt response of superoxide dismutase and peroxidase to dehydration and rehydration of the resurrection plant Haberlea rhodopensis. Plant Growth Regul 57:49–56
  • Zwiazek JJ (1991) Cell wall changes in white spruce (Picea glauca) needles subjected to repeated drought stress. Physiol Plant 82:513–518
Rekord w opracowaniu
Typ dokumentu
Identyfikator YADDA
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.