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2015 | 18 | 2 |
Tytuł artykułu

Variability of non-structural proteins of equine arteritis virus during persistent infection of the stallion

Treść / Zawartość
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
The genetic stability of ORF1a encoding non-structural proteins nsp1, nsp2, nsp3 and nsp4 of equine arteritis virus (EAV) has been analysed for nearly seven years in a persistently infected stallion of the Malopolska breed. Between November 2004 and June 2011, 11 semen samples were collected. Viral RNA extracted from semen of this carrier stallion was amplified, sequenced and compared with the sequences of the other known strains of EAV. Sequence analysis of ORF1a showed 84 synonymous and 16 non-synonymous mutations. The most variable part of ORF1a was the region encoding nsp2 protein with 13 non-synonymous substitutions. The degree of amino acid identity between isolates ranged from 98.91 to 100%. Only single non-synonymous mutations were detected in nsp1 (one substitution) and nsp4 (two substitutions). The most stable was nsp3 in which no amino acid substitutions were observed during the whole period of observation.
Słowa kluczowe
Wydawca
-
Rocznik
Tom
18
Numer
2
Opis fizyczny
p.255-259,fig.,ref.
Twórcy
autor
  • National Veterinary Research Institute, Partyzantow 57, 24-100 Pulawy, Poland
autor
  • National Veterinary Research Institute, Partyzantow 57, 24-100 Pulawy, Poland
  • National Veterinary Research Institute, Partyzantow 57, 24-100 Pulawy, Poland
Bibliografia
  • Balasuriya UB, Go YY, MacLachlan NJ (2013) Equine arteritis virus. Vet Microbiol 167: 93-122.
  • Balasuriya UB, Hedges JF, Smalley VL, Navarrette A, McCollum WH, Timoney PJ, Snijder EJ, MacLachlan NJ (2004) Genetic characterization of equine arteritis virus during persistent infection of stallions. J Gen Virology 85: 379-390.
  • Barrette-Ng IH, Ng KK, Mark BL, Van Aken D, Cherney MM, Garen C, Kolodenko Y, Gorbalenya AE, Snijder EJ, James MN (2002) Structure of arterivirus nsp4. The smallest chymotrypsin-like proteinase with an alpha/beta C-terminal extension and alternate conformations of the oxyanion hole. J Biol Chem 277: 39960-39966. de Vries AA, Chirnside ED, Horzinek MC, Rottier PJ (1992) Structural proteins of equine arteritis virus. J Virol 66: 6294-6303.
  • Firth AE, Zevenhoven-Dobbe JC, Wills NM, Go YY, Balasuriya UB, Atkins JF, Snijder EJ, Posthuma CC (2011) Discovery of a small arterivirus gene that overlaps the GP5 coding sequence and is important for virus production. J Gen Virology 92: 1097-1106.
  • Holyoak GR, Balasuriya UB, Broaddus CC, Timoney PJ (2008) Equine viral arteritis: current status and prevention. Theriogenology 70: 403-414.
  • Liu L, Castillo-Olivares J, Davis-Poynter NJ, Baule C, Xia H, Belhk S (2008) Analysis of ORFs 2b, 3, 4, and partial ORF5 of sequential isolates of equine arteritis virus shows genetic variation following experimental infection of horses. Vet Microbiol 129: 262-268.
  • Miszczak F, Legrand L, Balasuriya UB, Ferry-Abitbol B, Zhang J, Hans A, Fortier G, Pronost S, Vabret A (2012) Emergence of novel equine arteritis virus (EAV) variants during persistent infection in the stallion: origin of the 2007 French EAV outbreak was linked to an EAV strain present in the semen of a persistently infected carrier stallion. Virology 423: 165-174.
  • Patton JF, Balasuriya UB, Hedges JF, Schweidler TM, Hul-linger PJ, MacLachlan NJ (1999) Phylogenetic characterization of a highly attenuated strain of equine arteritis virus from the semen of a persistently infected standardbred stallion. Arch Virol 144: 817-827.
  • Rola J, Larska M, Rola JG, Belhk S, Autorino GL (2011) Epizotiology and phylogeny of equine arteritis virus in hucul horses. Vet Microbiol 148: 402-407.
  • Snijder EJ, Kikkert M, Fang Y (2013) Arterivirus molecular biology and pathogenesis. J Gen Virol 94: 2141-2163.
  • Surma-Kurusiewicz K, Winiarczyk S, Adaszek Ł (2013) Comparative analysis of ORF5 nucleotide sequences and amino acid sequences of the GP5 protein of equine arteritis virus (EAV) detected in the semen of stallions from Eastern Poland. Res Vet Sci 94: 361-367.
  • Timoney PJ, McCollum WH, Roberts AW, Murphy TW (1986) Demonstration of the carrier state in naturally acquired equine arteritis virus infection in the stallion. Res Vet Sci 41: 279-280.
  • van Aken D, Zevenhoven-Dobbe J, Gorbalenya AE, Snijder EJ (2006) Proteolytic maturation of replicase polyprotein pp1a by the nsp4 main proteinase is essential for equine arteritis virus replication and includes internal cleavage of nsp7. J Gen Virol 87: 3473-3482.
  • van den Born E, Gultyaev AP, Snijder EJ (2004) Secondary structure and function of the 5'-proximal region of the equine arteritis virus RNA genome. RNA 10: 424-437.
  • Wieringa R, de Vries AA, Raamsman MJ, Rottier PJ (2002) Characterization of Two New Structural Glycoproteins, GP(3) and GP(4), of Equine Arteritis Virus. J Virol 76: 10829-10840.
  • Zhang J (2005) Permissiveness of selected cell lines to equine arteritis virus: establishment, characterization, and significance of persistent infection in HeLa cells. University of Kentucky doctoral dissertations.
  • Zhang J, Go YY, MacLachlan NJ, Meade BJ, Timoney PJ, Balasuriya UB (2008) Amino acid substitutions in the structural or nonstructural proteins of a vaccine strain of equine arteritis virus are associated with its attenuation. Virology 378: 355-362.
  • Ziebuhr J, Snijder EJ, Gorbalenya AE (2000) Virus-encoded proteinases and proteolytic processing in the Nidovirales. J Gen Virol 81: 853-879.
Typ dokumentu
Bibliografia
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Identyfikator YADDA
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