Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2011 | 51 | 4 |

Tytuł artykułu

Utilization of carbohydrates by Beauveria bassiana isolates obtained from forest pests

Warianty tytułu

Języki publikacji



Carbohydrate utilization profiles of ten isolates of the entomopathogenic fungus Beauveria bassiana (Bals.-Criv.) Vuillemin were studied and compared to assist in determining their phenotypic characterization. Isolates were obtained in pure cultures from dead individuals of several forest pests collected from different regions in Bulgaria. Studies on utilization profiles were based on the acidification of twenty carbohydrates. The results indicate that sucrose, maltose and trehalose were assimilated at a high degree compared to esculin, arabinose and dulcitol. According to the results of a cluster analysis of the carbohydrate utilization profiles, the B. bassiana isolates were divided into two larger groupings. All isolates in the first larger cluster were obtained from the coleopteran insects - Stenomax aeneus, Ips typographus, I. sexdentatus and Dryocoetes autographus. Isolates from the other cluster were obtained from the lepidopteran larvae of Thaumetopoea pityocampa and Lymantria dispar, and from an adult of the coleopteran species Hylurgops palliatus. We determined that each B. bassiana isolate exhibited a different and specific carbohydrate utilization profile but differences at the p-level < 0.05 were significant among some of them. The most distinguishable was the isolate 560Bb obtained from T. pityocampa. Differences between the isolate 560Bb and the other nine B. bassiana isolates were highly significant at the p-level < 0.005. Isolate 433Bb obtained from a dead adult of I. typographus was significantly different from five of the studied isolates at the p-level < 0.05.

Słowa kluczowe








Opis fizyczny



  • Plant Protection Institute, 35 Panajot Volov Str., 2230 Konstinbrod, Bulgaria


  • Becker Z.E. 1988. Physiology and Biochemistry of Fungi. Moscow University Press, Moscow, 230 pp.
  • Bidochka M.J., Low N.H., Khachatourians G.G. 1990. Carbohydrate storage in the entomopathogenic fungus Beauveria bassiana. Appl. Environ. Microbiol. 56 (10): 3186-3190.
  • Bridge P.D., Abraham Y.J., Cornish M.C., Prior C., Moore D. 1990. The chemotaxonomy of Beauveria bassiana (Deuteromycotina: Hyphomycetes) isolates from the coffee berry borer Hypothenemus hampei (Coleoptera: Scolytidae). Mycopathologia 111 (2): 85-90.
  • Bridge P.D., Williams M.A.J., Prior C., Paterson R.R.M. 1993. Morphological, biochemical and molecular characteristics of Metarhizium anisopliae and Metarhizium flavoviride. J. Gen. Microbiol. 139 (6): 1163-1169. Doi: 10.1099/00221287-139-6-1163
  • Campbell R.K., Barnes G.L., Cartwright B.O., Eikenbary R.D. 1983. Growth and sporulation of Beauveria bassiana and Metarhizium anisopliae in a basal medium containing various carbohydrate sources. J. Invertebr. Pathol. 41 (1): 117-121.
  • Draganova S. 1997. Utilization of carbon sources by the Beauveria Vuill. genus (Moniliales, Deuteromycotina). Bulg. J. Agric. Sci. 3: 557-562.
  • Draganova S., Lecheva I. 2002. Discrimination between Beauveria bassiana strains isolated from Agriopis bajaria (Lepidoptera: Geometridae). Bulg. J. Agric Sci. 8: 43-48.
  • Draganova S., Trenchev G. 1993. A highly virulent strain of Beauveria bassiana (Bals.) Vuill. on the larvae of Agrilus ribesi Schaeff. (Coleoptera: Buprestidae). p. 241-245. In: Proc. the Second National Scientific Entomological Conference (G. Tsankov, ed.). PSSA, Sofia.
  • El-Sayed G.N., Ignoffo C.M., Leathers T.D., Gupta S.C. 1992. Use of a colorimetric system to detect enzymes expressed by germinating conidia of entomopathogenic fungi. Mycopathologia 118 (1): 29-36.
  • Evlakhova A.A. 1974. Entomogenous Fungi. Classification, Biology, Practical Significance. "Nauka" Press, Leningrad, 260 pp.
  • Kretovich V.L. 1986. Biochemistry of Plants. University Press, Moscow, 503 pp.
  • Lanyi B. 1987. Classical and rapid identification methods for medically important bacteria. Methods Microbiol. 19: 1-16.
  • Lederkremer R.M., Gallo-Rodriguez C. 2004. Naturally occurring monosaccharides. Properties and Synthesis. Adv. Carbohydrate Chem. Biochem. 5: 9-69.
  • Mugnai L., Bridge P.D., Evans H.C. 1989. A chemotaxonomic evaluation of the genus Beauveria. Mycol. Res. 92 (2): 199-209. Doi: 10.1016/S0953-7562(89)80012-7
  • Ownley B.H., Griffin M.R., Klingeman W.E., Gwinn K.D., Moulton J.K., Pereira R.M. 2008. Beauveria bassiana: Endophytic colonization and plant disease control. J. Invertebr. Pathol. 98 (3): 267-270. Doi: 10.1016/j.jip.2008.01.010
  • Pernfuss B., Schweigkofler W., Strasser H. 2003. Distinction of the entomopathogenic fungal species Beauveria brongniartii and Beauveria bassiana by comparing their carbon utilization patterns. In: "Entomopathogens and Insect Parasitic Nematodes: Current Research and Perspectives in Pest Biocontrol" (B. Papierok, ed.). IOBC/WPRS Bull. 26 (1): 121-123.
  • Posada F., Vega F.E. 2005. Establishment of the fungal entomopathogen Beauveria bassiana (Ascomycota: Hypocreales) as an endophyte in cocoa seedlings (Theobroma cacao). Mycologia 97 (6): 1195-1200.
  • Rath A.C., Carr C.J., Graham B.R. 1995. Characterization of Metarhizium anisopliae strains by carbohydrate utilization (API 50 CH). J. Invertebr. Pathol. 65 (2): 152-161.
  • St. Leger R.J., Charnley A.K., Cooper R.M. 1986. Enzymatic characterization of entomopathogens with the APIZYM system. J. Invertebr. Pathol. 48 (3): 375-376.
  • Sun M.H., Liu X.Z. 2006. Carbon requirements of some nematophagous, entomopathogenic and mycoparasitic Hyphomycetes as fungal biocontrol agents. Mycopathologia 161 (5): 295-305. Doi: 10.1007/s11046-006-0249-9
  • Todorova S., Cote J.C., Coderre D. 1998. Distinction between Beauveria and Tolypocladium by carbohydrate utilization. Mycol. Res. 102 (1): 81-87.
  • Todorova S., Cote J.C., Martel P., Coderre D. 1994. Heterogeneity of two Beauveria bassiana strains revealed by biochemical tests, protein profiles and bioassays on Leptinotarsa decemlineata (Coleoptera: Chrysomelidae) and Coleomegilla maculate lengi (Coleoptera: Coccinellidae) larvae. Entomophaga 39 (2): 159-169.
  • Vega F.E., Posada F., Aime M.C., Pava-Ripoll M., Infante F., Rehner S.A. 2008. Entomopathogenic fungal endophytes. Biol. Control 46 (1): 72-82. Doi: 10.1016/j.biocontrol.2008.01.008


Rekord w opracowaniu

Typ dokumentu



Identyfikator YADDA

JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.