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2015 | 14 | 1 |
Tytuł artykułu

Assessment of membrane stabilizing activity from honey. An in-vitro approach

Treść / Zawartość
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
Aim. The present study was conducted to evaluate Manoflora (MF), Polyflora (PF), Polyflora forest (PFf), and Processed (Pro) honey varieties to compare the in-vitro anti-inflammatory effects of aqueous honey samples in dose dependent manner. In-vitro anti-inflammatory activity was evaluated using membrane stabilization assay of RBCs at different aqueous honey concentrations. Material and method. The present investigation carried out for selected varieties of honey against erythrocytes exposed to both heat and hypotonic lyses and inhibition of membrane damage was compared to the standard drug acetylsalicylic acid. Results. Membrane damage was inhibited in both the model hemolysis of erythrocytes in vitro in a concentration dependent manner. Hypotonic solution inducing damage was inhibited by aqueous honey sample in ascending order ranged from 8.25% to 97.76% at 10 to 50 mg/ml and standard drug acetylsalicylic acid showing hemolysis protection 96.09% at 100 pg/ml concentration. In heat induced hemolysis model aqueous honey sample exhibited its protecting property during external stress condition in all samples ranged from 0.44% to 21.23% at 10 to 50 mg/ml and acetylsalicylic acid showed 39.38% at 100 pg/ml concentration. Among the variety PFf showed highest protecting nature for hypotonic solution induced lyses (97.76%) and heat induced hemolysis (21.23%) at 50 mg/ml respectively. Conclusion. With these investigations data conclude that the model exhibits marked anti-inflammatory effect. Future research is to be carried out to identify the molecules responsible in honey and its mechanism involved.
Słowa kluczowe
Wydawca
-
Rocznik
Tom
14
Numer
1
Opis fizyczny
p.85-90,fig.,ref.
Twórcy
  • Department of Studies in Biotechnology, University of Mysore, Manasagangothri Mysore-570006, Karnataka, India
autor
  • Department of Studies in Biotechnology, University of Mysore, Manasagangothri Mysore-570006, Karnataka, India
Bibliografia
  • Ahmadiani, A., Fereidoni, M., Semnanian, S., Kamalinejad, M., Saremi, S. (1998). Antinociceptive and anti-inflammatory effects of Sambucus ebulus rhizome extract in rats. J. Ethnopharm., 2, 229-232.
  • Al-Mamary, M., Al-Meeri, A., Al-Habori, M. (2002). Antioxidant ctivities and total phenolics of iflferent types of honey. Nutr. Res. 22, 1041-1047.
  • Chou, C. T. (1997). The anti-inflammatory effect of Tripterygium wilfordii Hook F on adjuvant Induced paw edema in rats and inflammatory mediators release. Phy- tother. Res., 11, 152-154.
  • Definition of honey and honey products. Approved by the National Honey Board June 15, 1996. Updated September 27, 2003. (2003). [online], www.honey.com.images/ uploads/general/honeydefs.pdf.
  • Gandhisan, R., Thamaraichelvasn, A., Baburaj, S. (1991). Anti-inflammatory action of Lannea coromandelica HRBC membrane stabilization. Fitoterapia, 62, 82-83.
  • Grossman, R. (1986). The other medicines: the penicillin of bees. A Pan Books 177. London: MacMillan.
  • Kumarappan, C. T., Chandra, R., Mandal. S. C. (2006). Anti-inflammatory activity of Ichnocarpus frutescens. Pharmacologyonline, 2, 201-206.
  • Molan, P. C. (2002). Reintroducing honey in the management of wounds and ulcers: Theory and practice. Ostomy Wound Manag., 48, 28—40.
  • Murugasan, N., Vember, S., Damodharan, C. (1981). Studies on erythrocyte membrane IV. In vitro haemolytic activity of Oleander extract. Toxicol. Lett., 8, 33-38.
  • New medical dictionary. (2007). New Delhi: Oxford and IBH Publ.
  • Opie, E. L. (1962). On the relation of necrosis and inflammation to dénaturation of proteins. J. Exp. Med., 115, 597-608.
  • Perez, R. M., Perez S., Zavala, M. A., Salazar, M. (1995). Anti-inflammatory activity of the bark of Hippocratea excelsa. J. Ethnopharm., 47, 85-90.
  • Rakhi, K. Chute, Deogade, N. G., Meghna Kawale (2010). Antimicrobial activity of Indian honey against clinical isolates. Asiatic J. Biotech. Res., 1, 35-38.
  • Sadique, J. A., Al-Rqobah, W. A., Bugharith, M. E., El-Gin- dy, A. R. (1989). The bioactivity of certain medicinal plants on the stabilization of RBC membrane system. Fitoterapia, 6, 525-532.
  • Shinde, U. A., Phadke, A.S., Nair, A.M., Mugantiwar, A.A., Dikshit, V.J., Saraf, V.O. (1999). Membrane stabilizing activity - a possible mechanism of action for the antiinflammatory activity of Cedrus deodara wood oil. Fitoterapia, 70 (3), 251-257.
  • Tripathi, K. D., (2008). Essentials of medical pharmacology. New Delhi: Jaypee Brothers Medical Publ.
  • White, J.W. Jr. (1980). Detection of honey adulteration by carbohydrate analysis. AOAC, 63(1), 11-18.
  • Umapathy, E., Ndebia, E. J., Meeme, A., Adam, B., Men- ziura, P., Nkeh-Chungag, B. N., Iputo, J. E. (2010). An experimental evaluation of Albuca setosa aqueous extract on membrane stabilization, protein dénaturation and white blood cell migration during acute inflammation. J. Med. Plant Res., 4 (9), 789-795.
  • White, J. W. Jr. (1962). Composition of American honeys. Tech. Bull. 1261. Washington, DC: Agric. Res. Serv. USDA.
  • Vadivu, R., Lakshmi, K. S. (2008). In-vitro and in-vivo antiinflammatory activity of leaves of Symplocos cochinchnensis (Lour) Moore ssp. laurina. Bangl. J. Pharmacol., 3. 121-124.
Typ dokumentu
Bibliografia
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