Comparison of serum protein profiles of Borrelia burgdorferi-positive Bernese Mountain Dogs and dogs of other breeds using MALDI-TOF technique

• Autorzy: Pisarek M. , Kalinowski M. , Skrzypczak M. , Mazurek L. , Michalak K. , Pietras-Ozga D. , Dokuzeylu B. , Winiarczyk S. , Adaszek L.
• Języki publikacji: EN

Abstrakty

EN

The aim of the study was to compare the serum protein profile of Bernese Mountain Dogs (BMDs) reacting positive for Bb in snap testing with the serum protein profile of dogs of other breeds (healthy and with clinical borreliosis) using the MALDI time-of-flight (MALDI-TOF) technique. The observations included five groups of dogs. BMDs reacting positively to Bb in snap serological testing and showing symptoms of borreliosis (group 1), BMDs for which no borreliosis symptoms were determined but with seropositivity for Bb determined with snap serological tests (group 2), clinically healthy BMDs with no antibodies for Bb found in the serum (group 3), five dogs of different breeds, reacting positively in serological testing, in which borreliosis symptoms were observed (group 4), clinically healthy dogs of different breeds with negative reaction in tests towards Bb (group 5). A proteomic analysis demonstrated the presence of five identical protein fractions among all five groups. An additional two protein fractions of approximately 7.630 and 15.260 kDa were found in all the serum samples obtained from the dogs positive for borrelia in a snap test, both in those exhibiting symptoms of borreliosis, and seropositive BMDs not presenting symptoms of the disease. These two additional protein fractions may be used to differentiate between seropositive and seronegative B. burgdorferi dogs and may be considered a seropositivity marker, however, it cannot be used to differentiate between animals with the clinical form of the disease and those that are only seropositive.

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 2023
• Tom: 26
• Numer: 3
• Opis fizyczny: p.493-496,fig.,ref.

Twórcy

autor

Pisarek M.

• Department of Epizoology and Clinic of Infectious Diseases, Faculty of Veterinary Medicine of the University of Life Sciences in Lublin, ul. Głęboka 30, 20-612 Lublin, Poland

autor

Kalinowski M.

• Department of Epizoology and Clinic of Infectious Diseases, Faculty of Veterinary Medicine of the University of Life Sciences in Lublin, ul. Głęboka 30, 20-612 Lublin, Poland

autor

Skrzypczak M.

• Second Department of Gynecology, Medical University of Lublin, 20-954 Lublin, Poland

autor

Mazurek L.

• Department of Epizoology and Clinic of Infectious Diseases, Faculty of Veterinary Medicine of the University of Life Sciences in Lublin, ul. Głęboka 30, 20-612 Lublin, Poland

autor

Michalak K.

• Department of Epizoology and Clinic of Infectious Diseases, Faculty of Veterinary Medicine of the University of Life Sciences in Lublin, ul. Głęboka 30, 20-612 Lublin, Poland

autor

Pietras-Ozga D.

• Department of Epizoology and Clinic of Infectious Diseases, Faculty of Veterinary Medicine of the University of Life Sciences in Lublin, ul. Głęboka 30, 20-612 Lublin, Poland

autor

Dokuzeylu B.

• Department of Internal Medicine, Veterinary Faculty, Istanbul University-Cerrahpasa, 34320 Avcilar Campus, Avcilar, Istanbul, Turkey

autor

Winiarczyk S.

• Department of Epizoology and Clinic of Infectious Diseases, Faculty of Veterinary Medicine of the University of Life Sciences in Lublin, ul. Głęboka 30, 20-612 Lublin, Poland

autor

Adaszek L.

• Department of Epizoology and Clinic of Infectious Diseases, Faculty of Veterinary Medicine of the University of Life Sciences in Lublin, ul. Głęboka 30, 20-612 Lublin, Poland

Identyfikatory

DOI

10.24425/pjvs.2023.145041