Prevalence of Helicobacter pullorum in Egyptian hen’s eggs and in vitro susceptibility to different antimicrobial agents

• Autorzy: Hameed K.G.A. , Sender G.
• Języki publikacji: EN

Abstrakty

EN

The objective of the study was to investigate the occurrence of H. pullorum in chicken hens’ eggs and to determine their in vitro susceptibility to various antimicrobial agents. A total of 300 fresh commercial chicken eggs of Balady native breed and poultry farms (PF) were collected from both Assiut and Qena Governorates in Egypt. Every five eggs represented one sample (mixed contents of five eggs). The eggshell and eggshell contents were examined for the presence of Helicobacter species by conventional methods, next confirmed with PCR. The highest incidence of contamination with Helicobacter species was recorded in eggs from Assiut Governorate poultry farms. The obtained results of Helicobacter pullorum (H. pullorum) isolation revealed that poultry farms egg shells and contents were less contaminated in both Governorates than Balady ones. Additionally, fresh egg contents of poultry farms in both Governorates were free from H. pullorum. All 12 isolates that were conventionally classified as carrying H. pullorum occurred free from the organism when tested with PCR method by detection of one PCR product on agarose gel that matched the predicted size of 477 bp that corresponding to 16S rRNA region of the gene. H. pullorum show in vitro susceptibility to almost all tested types of antibiotics except for ampicillin, ceftriaxone, and sulphamethoxazole trimethoprim.

Słowa kluczowe

EN

prevalence; Helicobacter pullorum; Egyptian hen; hen; hen egg; in vitro; susceptibility; antimicrobial agent

• Wydawca: -
• Czasopismo: Animal Science Papers and Reports
• Rocznik: 2011
• Tom: 29
• Numer: 3
• Opis fizyczny: p.257-264,fig.,ref.

Twórcy

autor

Hameed K.G.A.

• South Valley University, Qena, Egypt

autor

Sender G.

Bibliografia

• ATABAY H.I., CORRY J.E.L., ON S.L.W., 1998 − Identification of unusual Campylobacter-like isolates from poultry products as Helicobacter pullorum. Journal of Applied Microbiology 84, 1017-1024.
• BAELE M., VAN DEN BULCK K., DECOSTERE A., VANDAMME P., HÄNNINEN M.-L.,DUCATELLE R., HAESEBROUCK F., 2004 − Multiplex PCR assay for differentiation of Helicobacter felis, H. bizzozeronii, and H. salomonis. Journal of Clinical Microbiology 42, 1115-1122.
• BURNENS A.P., STANLEY J., MORGENSTERN R., NICOLET J. 1994 − Gastroenteritis associated with Helicobacter pullorum. Lancet 344, 1569-1570.
• CEELEN L., DECOSTERE A., ON S.W.L., VAN DEN BULCK K., BAELE M., DUCATELLE R.,HAESEBROUCK F. 2006 − Helicobacter pullorum in chickens, Belgium. Emerging Infectious Diseases 12, 263-267.
• CEELEN L., DECOSTERE A., VERSCHRAEGEN G., DUCATELLE R., HAESEBROUCK F., 2005 − Prevalence of Helicobacter pullorum among patients with gastrointestinal disease and clinically healthy persons. Journal of Clinical Microbiology 43, 2984-2986.
• CHAVES S., GADANHO M., TENREIRO R., AND CABRITA J., 1999 − Assessment of metronidazole susceptibility in H. pylori: statistical validation and error rate analysis of breakpoints determined by the disc diffusion test. Journal of Clinical Microbiology 37, 1628-1631.
• GIBSON J.R., FERRUS M.A., WOODWARD D., XERRY J., OWEN R.J., 1999 − Genetic diversity In Helicobacter pullorum and poultry sources identified by an amplified fragment length polymorphism technique and pulsed-field gel electrophoresis. Journal of Applied Microbiology 87, 602-610.
• MELITO P.L., WOODWARD D.L., BERNARD K.A., PRICE L., KHAKHRIA R., JOHNSON W.M., RODGERS F.G., 2000 − Differentiation of clinical Helicobacter pullorum isolates from related Helicobacter and Campylobacter species. Helicobacter 5, 142-147.
• MOATS W.A., 1980 − Classification of bacteria from commercial egg washers and washed and unwashed eggs. Journal of Applied Environmental Microbiology 4, 710-714.
• NATIONAL COMMITTEE FOR CLINICAL LABORATORY STANDARDS (NCCLS). 2002 −Approved standard, second edition M31-A2. Selection of antimicrobial agents for routine testing and reporting.
• ON S.L.W., HOLMES B., SACKIN M.J., 1996 − A probability matrix for the identification of campylobacters, helicobacters and allied taxa. Journal of Applied Bacteriology 81, 425-432.
• ON S.L.W., HYNEST S., WADSTROM T., 2002 − Extragastric Helicobacter species. Helicobacter 7, S63-67.
• SCHMITZ F.J., MACKENZIE C.R., HOFMANN B., VERHOEF J., FRINKEN EIGEN M., HEINZ H.P., KOHRER K., 1997 − Specific information concerning taxonomy, pathogenicity and methicillin resistance of staphylococci obtained by multiplex PCR. Journal of Medical Microbiology 46, 773-778.
• Speck M.L., 1976 − Compendium of Methods for Microbiological Examination of Food. American Public Health Association, Washington.
• STANLEY J., LINTON D., BURNENS A.P., DEWHIRST F.E., ON S.L.W., PORTER A., OWEN R.J.,COSTAS M., 1994 − Helicobacter pullorum species novel genotype and phenotype of a new species isolated from poultry and from human patients with gastroenteritis. Microbiology 140, 3441- 3449.
• STEINBRUECKNER B., HEARTER G., PELZ K., WEINER S., RUMP J.A., DEISSLER W.,1997 − Isolation of Helicobacter pullorum from patients with enteritis. Scandinavian Journal of Infectious Diseases 29, 315-318.
• YOUNG V.B., CHIEN C.C., KNOX K.A., TAYLOR N.S., SCHAUER D.B., FOX J.G. 2000 − Cytolethal distending toxin in avian and human isolates of Helicobacter pullorum. Journal of Infectious Diseases 182, 620-623