Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2014 | 17 | 1 |

Tytuł artykułu

Using of immunocytochemistry in differential diagnosis of neoplasms of serosal cavities in dogs

Treść / Zawartość

Warianty tytułu

Języki publikacji



The presence of tumor within the serosal cavities, often connected with accumulation of serosal effusion, is a quite common problem in the small animal veterinary medicine. The first step in diagnosis of such cases is cytopathological examination. The aim of the present study was to evaluate the usefulness of cytology and immunocytochemistry, using commercially available antibodies (anti-cytokeratin, anti-vimentin, and anti-desmin), in differential diagnosis of malignant tumors located within serosal cavities in dogs. The final cytological diagnosis of carcinoma/adenocarcinoma, sarcoma, and mesothelioma was obtained on the basis of routine cytopathology and immunocytochemistry, and then confirmed by histopathology and immunohistochemistry. Cytoplasmic immunoreactivitiy of normal mesothelid cells and cytoplasmic immunoreactivity of hyperplastic mesothelial cells revealed constant and strong expression of all examined intermediate filaments: cytokeratin, vimentin and desmin. Application of routine cytopathology and immunocytochemistry allowed 32 neoplastic tumors to be detected: 19 cases of carcinomas/adenocarcinomas, 6 cases of sarcomas, 7 cases of mesotheliomas. Immunostaining of cytopathological samples with chosen set of antibodies: anti-cytokeratin, anti-vimentin, anti-desmin is a useful, and low invasive test for differentiation between mesotheliomas and carcinomas/adenocarcinomas in dogs.

Słowa kluczowe








Opis fizyczny



  • Division of Animal Pathomorphology, Department of Pathology and Veterinary Diagnostics, Faculty of Veterinary Medicine, Warsaw University of Life Sciences-SGGW, Nowoursynowska 159c, 02-766 Warsaw, Poland
  • Division of Animal Pathomorphology, Department of Pathology and Veterinary Diagnostics, Faculty of Veterinary Medicine, Warsaw University of Life Sciences-SGGW, Nowoursynowska 159c, 02-766 Warsaw, Poland


  • Baker R, Lumsden JH (2000) Pleural and peritoneal fluids. In: Baker R, Lumsden JH (eds) Color Atlas of Cytology of the Dog and Cat, Mosby, St Louis, pp 161-162.
  • Brisson BA, Reggeti F, Bienzle D (2006) Portal site metastasis of invasive mesothelioma after diagnostic thoracoscopy in a dog. J Am Vet Med Assoc 229: 980-983.
  • Bertazzolo W, Bonfanti U, Mazzotti S, Gelain ME (2012) Cytologic features and diagnostic accuracy of analysis of effusions for detection of ovarian carcinoma in dogs. Vet Clin Pathol 41: 127-132.
  • Burgess HJ, Kerr ME (2009) Cytokeratin and vimentin co-expression in 21 canine primary pulmonary epithelial neoplasms. J Vet Diagn Invest 21: 815-820.
  • Caniatti M, Roccabianca P, Scanziani E, Paltrieri S, Moore PF (1996) Canine lymphoma: immunocytochemical analysis of fine-needle aspiration biopsy. Vet Pathol 33: 204-212.
  • Charney SC, Bergman PJ, McKnight JA, Ferelly J, Novosad CA, Leibman NF, Camps-Palau MA (2005) Evaluation of intracavitary mitoxantrone and carboplatin for treatment of carcinomatosis, sarcomatosis and mesothelioma, with or without malignant effusions: A retrospective analysis of 12 cases (1997-2002). Vet Comp Oncol 3: 171-181.
  • Desnoyers MM, Haines DM, Searcy GP (1990) Immunohistochemical detection of intermediate filament proteins in formalin fixed normal and neoplastic canine tissues. Can J Vet Res 54: 360-365.
  • Espino L, Vazquez S, Fáılde D, Barreiro A, Miño N, Goicoa A (2010) Localized pleural mesothelioma causing cranial vena cava syndrome in a dog. J Vet Diagn Invest 22: 309-312.
  • Fisher C (1990) The value of electronmicroscopy and immunohistochemistry in the diagnosis of soft tissue sarcomas: a study of 200 cases. Histopathology 16: 441-454.
  • Geninet C, Bernex F, Rakotovao F, Crespeau FL, Parodi AL, Fontaine JJ (2003) Sclerosing peritoneal mesothelioma in a dog - a case report. J Vet Med A Physiol Pathol Clin Med 50: 402-405.
  • Glińska K (2009) Evaluation of the usefulness of abdominal fluid examination in diagnosing the etiology of ascites in dogs. Med Weter 65: 40-45.
  • Grieco V, Patton V, Romussi S, Finazzi M (2003) Cytokeratin and vimentin expression in normal and neoplastic canine prostate. J Comp Pathol 129: 78-84.
  • Gumber S, Fowlkes N, Cho DY (2011) Disseminated sclerosing peritoneal mesothelioma in a dog. J Vet Diagn Invest 23: 1046-1050.
  • Höinghaus R, Mischke R, Hewicker-Trautwein M (2002) Use of immunocytochemical techniques in canine melanoma. J Vet Med A Physiol Pathol Clin Med 49: 198-202.
  • Höinghaus R, von Wasielewski R, Hewicker-Trautwein M, Freund M, Mischke R (2007) Immunocytological detection of lymph node metastases in dogs with malignant epithelial tumours. J Comp Pathol 137: 1-8.
  • Höinghaus R, Hewicker-Trautwein M, Mischke R (2008) Immunocytochemical differentiation of canine mesenchymal tumors in cytologic imprint preparations. Vet Clin Pathol 37: 104-111.
  • Hurlimann J (1994) Desmin and neural marker expression in mesothelial cells and mesotheliomas. Hum Pathol 25: 753-757.
  • McColl Williamson M, Middleton DJ (1998) Cutaneous soft tissue tumors in dogs: classification, differentiation, and histogenesis. Vet Dermatol 9: 43-48.
  • Ordonez NG (2006) The diagnostic utility of immunohistochemistry and electron microscopy in distinguishing between peritoneal mesotheliomas and serous carcinomas: a comparative study. Mod Pathol 19: 34-48.
  • Pires MA, Seixas F, Palmeira C, Payan-Carreira R (2010) Histopathologic and immunohistochemical exam in one case of canine endometrial adenocarcinoma. Reprod Domest Anim 45: 545-549.
  • Rabanal RM, Else RW (1994) Immunohistochemical localisation of cytokeratin and vimentin intermediate filament proteins in canine mammary tumours. Res Vet Sci 56: 225-233.
  • Reggeti F, Brisson B, Ruotsalo K, Southorn E, Bienzle D (2005) Invasive epithelial mesothelioma in a dog. Vet Pathol 42: 77-81.
  • Riccardi E, Grieco V, Verganti S, Finazzi M (2007) Immunohistochemical diagnosis of canine ovarian epithelial and granulosa cell tumors. J Vet Diagn Invest 19: 431-435.
  • Sapierzyński R (2010) Practical aspects of immunocytochemistry in canine lymphomas. Pol J Vet Sc 13: 661-668.
  • Sato T, Miyoshi T, Shibuya H, Fujikura J, Koie H, Miyazaki Y (2005) Peritoneal biphasic mesothelioma in a dog. J Vet Med A Physiol Pathol Clin Med 52: 22-25.
  • Sato A, Torii I, Okamura Y, Yamamoto T, Nishigami, T, Kataoka TR, Song M, Hasegawa S, Nakano T, Kamei T, Tsujimura T (2010) Immunocytochemistry of CD146 is useful to discriminate between malignant pleural mesothelioma and reactive mesothelium. Mod Pathol 23: 1458-1466.
  • Sisson D, Thomas WP, Ruehl WW, Zinkl JG (1984) Diagnostic value of pericardial fluid analysis in the dog. J Am Vet Med Assoc 184: 51-55.
  • Smith DA, Hill FW (1989) Metastatic malignant mesothelioma in a dog. J Comp Pathol 100: 97-101.
  • Stepien RL, Whitley NT, Dubielzig RR (2000) Idiopathic or mesothelioma-related pericardial effusion: clinical findings and survival in 17 dogs studied retrospectively. J Small Anim Pract 41: 342-347.
  • Szczepulska-Wójcik E, Langfort R, Roszkowski-Sliz K (2007) A comparative evaluation of immunohistochemical markers for the differential diagnosis between malignant mesothelioma, non-small cell carcinoma involving the pleura, and benign reactive mesothelial cell proliferation. Pneumonol Alergol Pol 75: 57-69.
  • Tzipory L, Vernau KM, Sturges BK, Zabka TS, Highland MA, Petersen SA, Wisner ER, Moore PF, Vernau W (2009) Antemortem diagnosis of localized central nervous system histiocytic sarcoma in 2 dogs. J Vet Intern Med 23: 369-374.
  • VascellariM, Carminato A, Camali G, Melchiotti E, Mutinelli F (2011) Malignant mesothelioma of the tunica vaginalis testis in a dog: histological and immunohistochemical characterization. J Vet Diagn Invest 23: 135-139.

Typ dokumentu



Identyfikator YADDA

JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.