Suppressive effect of electromagnetic field on analgesic activity of tramadol in rats
Treść / Zawartość
The electromagnetic fields (EMFs) have been shown to alter animal and human behavior, such as directional orientation, learning, pain perception (nociception or analgesia) and anxiety-related behaviors. The aim of this study was to evaluate the influence of electromagnetic fields of high-frequency microwaves on pain perception and anti-nociceptive activity of tramadol (TRAM) - analgetic effective in the treatment of moderate to severe acute and chronic pain states. Electromagnetic fields exposures of a) 1500 MHz frequency and b) modulated, 1800 MHz (which is identical to that generated by mobile phones) were applied. Paw withdrawal latency (PWL) to thermal stimulus was measured in vehicle or tramadol (TRAM) treated animals before and after 30, 60 and 90 minutes from injections. The differences in the level of pain (PWL) between control group and rats exposed to EMF alone in three measurements, were not observed. Tramadol alone significantly increased PWLs to thermal stimulus in comparison to vehicle results at 30 (p < 0.001) and 60 minutes (p < 0.05) after drug injection. EMF exposure of both frequencies transiently suppressed analgesic effect of tramadol, significantly reducing paw withdrawal latency in animals treated with this drug at 30 minutes from the drug injection.
- Department of Microwave Safety, Military Institute of Hygiene and Epidemiology, Kozielska 4, 01-163 Warsaw, Poland
- Berkels R, Taubert D, Rosenkranz A, Rosen R (2003) Vascular protective effects of dihydropyridine calcium antagonists. Involvement of endothelial nitric oxide. Pharmacology 69: 171-176.
- Dixon SJ, Persinger MA (2001) Suppression of analgesia in rats induced by morphine or L-NAME but not both drugs by microTesla, frequency-modulated magnetic fields. Int J Neurosci 108: 87-97.
- Driessen B, Reimann W, Giertz H (1993) Effects of the central analgesic tramadol on the uptake and release of noradrenaline and dopamine in vitro. Br J Pharmacol 108: 806-11.
- Franzellitti S, Valbonesi P, Ciancaglini N, Biondi C, Contin A, Bersani F, Fabbri E (2010) Transient DNA damage induced by high-frequency electromagnetic fields (GSM 1.8 GHz) in the human trophoblast HTR-8/SVneo cell line evaluated with the alkaline comet assay. Mutat Res 683: 35-42.
- Gerner C, Haudek V, Schandl U, Bayer E, Gundacker N, Hutter HP, Mosgoeller W (2010) Increased protein synthesis by cells exposed to a 1,800-MHz radio-frequency mobile phone electromagnetic field, detected by proteome profiling. Int Arch Occup Environ Health 83: 691-702.
- Feng Y, Piletz JE, Leblanc MH (2002) Agmatine suppresses nitric oxide production and attenuates hypoxic-ischemic brain injury in neonatal rats. Pediatr Res 52: 606-611.
- Goeringer KE, Logan BK, Christian GD (1997) Identification of tramadol and its metabolites in blood from drug-related deaths and drug-impaired drivers. J Anal Toxicol 21: 529-537.
- Hara K, Minami K, Sata T (2005) The effects of tramadol and its metabolite on glycine, gamma-aminobutyric acidA, and N-methyl-D-aspartate receptors expressed in Xenopus oocytes. Anesth Analg 100: 1400-1405.
- Hargreaves K, Dubner R, Brown F, Flores C, Joris J (1988) A new and sensitive method for measuring thermal nociception in cutaneous hyperalgesia. Pain 32: 77-88.
- Lehmann KA (1997) Tramadol in acute pain. Drugs 53: 25-33.
- Martin LJ, Persinger MA (2005) The influence of various pharmacological agents on the analgesia induced by an applied complex magnetic field treatment: a receptor system potpourri. Electromagn Biol Med 24: 87-97.
- Minami K, Uezono Y, Ueta Y (2007) Pharmacological Aspects of the Effects of Tramadol on G-Protein Coupled Receptors. J Pharmacol Sci 103: 253-260.
- Monks R, Merskey H (1999) Psychotropic drugs. In: Wall PD, Melzack R (eds) Textbook of pain. Churchill Livingstone, London, pp 1155-1186.
- Nylund R, Leszczynski D (2004) Proteomics analysis of human endothelial cell line EA.hy926 after exposure to GSM 900 radiation. Proteomics 4: 1359-1365.
- Shupak NM, Prato FS, Thomas AW (2004) Human exposure to a specific pulsed magnetic field: effects on thermal sensory and pain thresholds. Neurosci Lett 363: 157-162.
- Raffa RB, Friderichs E, Reimann W, Shank RP, Codd EE, Vaught JL (1992) Opioid and nonopioid components independently contribute to the mechanism of action of tramadol, an ‘atypical’ opioid analgesic. J Pharmacol Exp Ther 260: 275-285.
- Sindrup SH, Andersen G, Madsen C, Smith T, Brøsen K, Jensen TS (1999) Tramadol relieves pain and allodynia in polyneuropathy: a randomised, double-blind, controlled trial. Pain 83: 85-90.
- Stankiewicz W, Dąbrowski MP, Kubacki R, Sobiczewska E, Szmigielski S (2006) Immunotropic influence of 900 MHz microwave GSM signal on human blood immune cells activated in vitro. Electromagn Biol Med 25: 45-51.
- Stankiewicz W, Zdanowski R, Skopińska-Różewska E, Ujazdowska D, Kieliszek J, Skopiński P, Bodera P, Sommer E (2011) The effect of 900 MHz microwave GSM Signal on the proliferation of endothelial cells In vitro. Centr Eur J Immunol 36: 215-219.
- Yang XC, Reis DJ (1999) Agmatine selectively blocks the N-methyl-D-aspartate subclass of glutamate receptor channels in rat hippocampal neurons. J Pharmacol Exp Ther 288: 544-549.
- Zhu S, Stavrovskaya IG, Drozda M, Kim BY, Ona V, Li M, Sarang S, Liu AS, Hartley DM, Wu DC, Gullans S, Ferrante RJ, Przedborski S, Kristal BS, Friedlander RM (2002) Minocycline inhibits cytochrome C release and delays progression of amyotrophic lateral sclerosis in mice. Nature 417: 74-78