Background: The aim of this study is to evaluate whether spinning exercise may have an impact on ovarian reserve capacity in reproductive young women. Material and methods: 48 healthy women were included into this prospective cohort study and randomly categorized into control (n = 14) and experimental groups (n = 12). Spinning exercise sessions were conducted for 50 minutes three times a week for 8 weeks in the study group. The control group did not exercise. Immediately before and after the spinning program, each participant underwent the measurements of the body mass index, the waist circumference, the hip circumference as well as serum anti-müllerian hormone (AMH), estradiol (EM), follicle stimulating hormone (FSH) and luteinizing hormone (LH) levels. Results: There were significant differences in both of group in terms of antropemetric features (body weight, BMI, fat percent, waist circumference and hip circumference). There was only a significant difference of AMH in the experimental group. Conclusions: Spinning exercise would be an important cause of associated with a decreased serum AMH level in reproductive young women. Further research should expand the finding by questioning whether intensity or practicing is an important mechanism in reproductive young women.