Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2011 | 33 | 5 |

Tytuł artykułu

Disturbance of ethylene biosynthesis and perception during somatic embryogenesis in Medicago sativa L. reduces embryos ability to regenerate

Warianty tytułu

Języki publikacji



Effects of non-specific ethylene biosynthesis inhibitors: salicylic acid (SA) and aminoethoxyvinylglycine (AVG), and of specific inhibitors of ethylene binding to receptors: 1-methylcyclopropene (1-MCP) and 2,5-norbornadiene (NBD) applied during proliferation and differentiation phases of indirect somatic embryogenesis (SE) of Medicago sativa L. cv. Rangelander on embryogenic suspension growth, embryo production, development, and ability to germinate and convert were studied. Application of SA and AVG alone or together at concentrations from 1 to 500 µM in B₅g liquid medium during the proliferation phase had an inhibitory effect on ethylene production and embryogenic suspension growth. Additionally, it caused a drastic reduction in production of embryos and their development on BOi2Y solid differentiation medium. The inhibitory effect of SA was more visible than that of AVG. In addition, disturbance of ethylene biosynthesis during the proliferation phase of SE resulted in diminished lateral germination and conversion of cotyledonary embryos on MS solid medium. Moreover, blocking of ethylene receptors by 1-MCP during the proliferation phase also inhibited ethylene production and embryogenic suspension growth and reduced embryo production during differentiation. MCP almost completely inhibited development of cotyledonary embryos. At the same time, development of more embryos was arrested at the globular stage, and the number of abnormal embryos almost doubled. Similarly, addition of 1-MCP or NBD to the ambient atmosphere during the differentiation phase evidently arrested the development of embryos and, consequently, their ability to germinate and convert on MS regeneration medium. All the results presented above demonstrated that not only ethylene biosynthesis, but also ethylene action is involved in the control of individual phases of SE in Medicago sativa L. cv. Rangelander. And what is more, disturbance of these processes during distinct phases of SE adversely affects vigor of the somatic embryos obtained.

Słowa kluczowe








Opis fizyczny



  • Department of Plant Biotechnology, University of Szczecin, Waska 13, 71-415, Szczecin, Poland
  • Department of Plant Biotechnology, University of Szczecin, Waska 13, 71-415, Szczecin, Poland


  • Abeles FB, Morgan PW, Saltveit ME (1992) Ethylene in plant biology. Academic Press, New York
  • Bingham ET, Hurley LV, Kaatz DM, Saunders JW (1975) Breeding alfalfa which regenerate from callus tissue culture. Crop Sci 15:719–721
  • El Meskaoui A, Tremblay FM (2001) Involvement of ethylene in the maturation of black spruce embryogenic cell lines with different maturation capacities. J Exp Bot 52:761–769
  • Gamborg OL, Miller RA, Oijma K (1968) Nutrient requirement of suspension cultures of soybean root cell. Exp Cell Res 50:148–151
  • Gomez-Jimenez MC, Matilla AJ, Garrido D (1998) Isolation and characterization of a DNA encoding an ACC-oxidase from Cicer arietinum and its expression during embryogenesis and seed germination. Austr J Plant Physiol 25:765–773
  • Harms K, Ramirez I, Péna-Cortés H (1998) Inhibition of woundinduced accumulation of allene oxide synthase transcript in flax leaves by aspirin and salicylic acid. Plant Physiol 118:1057–1065
  • Hatanaka T, Sawabe E, Azuma T, Uchida N, Yasuda T (1995) The role of ethylene in somatic embryogenesis from leaf discs of Coffea canephora. Plant Sci 107:199–204
  • Hays DB, Reid DM, Yeung EC, Pharis RP (2000) Role of ethylene in cotyledon development of microspore––derived embryos of Brassica napus. J Exp Bot 51:1851–1859
  • Huang XL, Xu JL, Gao DW, Ye WH, Li XJ (1998) The effect of MGBG on growth of the callus tissue, induction of somatic embryogenesis of alfalfa and their ethylene biosynthesis. Acta Bot Sin 40:635–641
  • Huang XL, Li XJ, Li Y, Huang LZ (2001) The effect of AOA on ethylene and polyamine metabolism during early phases of somatic embryogenesis in Medicago sativa. Physiol Plant 113:424–429
  • Johnson-Flanagan AM, Spencer MS (1994) Ethylene production during development of mustard (Brassica juncea) and canola (Brassica napus) seed. Plant Physiol 106:601–606
  • Kępczyńska E, Ruduś I, Kępczyński J (2009a) Endogenous ethylene in indirect somatic embryogenesis of Medicago sativa L. Plant Growth Regul 59:63–73
  • Kępczyńska E, Ruduś I, Kępczyński J (2009b) Abscisic acid and methyl jasmonate as regulators of ethylene biosynthesis during somatic embryogenesis of Medicago sativa L. Acta Physiol Plant 31:1263–1270
  • Kępczyński J, Kępczyńska E (1997) Ethylene in seed dormancy and germination. Physiol Plant 101:720–726
  • Kępczyński J, Kępczyńska E (2005) Manipulation of ethylene biosynthesis. Acta Physiol Plant 27:213–220
  • Kępczyński J, McKersie BD, Brown DCW (1992) Requirement of ethylene for growth of callus and somatic embryogenesis in Medicago sativa L. J Exp Bot 43:1199–1202
  • Kong L, Yeung EC (1994) Effects of ethylene and ethylene inhibitors on white spruce somatic embryo maturation. Plant Sci 104:71–80
  • Leslie CA, Romani RJ (1986) Salicylic acid: a new inhibitor of ethylene biosynthesis. Plant Cell Rep 5:144–146
  • Leslie CA, Romani RJ (1988) Inhibition of ethylene biosynthesis by salicylic acid. Plant Physiol 88:833–837
  • Mantiri FR, Kurdyukov S, Lohar DP, Sharapova N, Saeed NA, Wang XD, VandenBosch KA, Rose RJ (2008) The transcription factor MtSERF1 of the ERF subfamily identified by transcriptional profiling is required for somatic embryogenesis induced by auxin plus cytokinin in Medicago truncatula. Plant Physiol 146:1622–1636
  • Matilla AJ, Matilla–Vazquez MA (2008) Involvement of ethylene in seed physiology. Plant Sci 175:87–97
  • McKersie BD, Senaratna T, Bowley SR, Brown DCW, Krochko F, Bewley JD (1989) Application of artificial seed technology in the production of hybrid alfalfa (Medicago sativa L.). In Vitro Cell Dev Biol 25:1183–1188
  • Meijer EGM (1989) Developmental aspects of ethylene biosynthesis during somatic embryogenesis in tissue cultures of Medicago sativa L. J Exp Bot 40:479–484
  • Meijer EGM, Brown CW (1988) Inhibition of somatic embryogenesis in tissue cultures of Medicago sativa by aminoethoxyvinylglycine, amino-oxyacetic acid and 2,4-dinitrophenol and salicylic acid at concentrations which do not inhibit ethylene biosynthesis and growth. J Exp Bot 39:263–270
  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassay with tobacco callus cultures. Physiol Plant 15:473–497
  • Nascimento WM (2003) Ethylene and lettuce seed germination. Sci Agric 60:601–606
  • Nissen P (1994) Stimulation of somatic embryogenesis in carrot by ethylene: effects of modulators of ethylene biosynthesis and action. Physiol Plant 92:397–402
  • Pierik R, Tholen D, Poorter H, Visser EJW, Voesenek LACJ (2006) The Janus face of ethylene: growth inhibition and stimulation. Trends in Plant Sci 11:176–183
  • Rodrigez–Gacio MC, Nicolas C, Matilla AJ (2004) The final step of the ethylene biosynthesis pathway in turnip tops (Brassica rapa): molecular characterization of the 1-aminocyclopropane–1–carboxylate oxidase BrACO1 throughout zygotic embryogenesis and germination of heterogenous seeds. Physiol Plant 121:132–140
  • Roustan JP, Latche A, Fallot J (1990) Inhibition of ethylene production and stimulation of carrot embryogenesis by salicylic acid. Biol Plant 32:273–276
  • Ruduś I, Kępczyńska E, Kępczyński J (2006) Comparative efficiency of abscisic acid and metyl jasmonate for indirect somatic embryogenesis in Medicago sativa L. Plant Growth Regul 48:1–11
  • Ruduś I, Weiler EW, Kępczyńska E (2009) Do stress–related phytohormones, abscisic acid and jasmonic acid play a role in the regulation of Medicago sativa L. somatic embryogenesis? Plant Growth Regul 59:159–169
  • Schenk RU, Hildebrandt AC (1972) Medium and techniques for induction and growth of monocotyledonous and dicotyledonous plant cell cultures. Can J Bot 50:199–204
  • Smalle J, Van Der Straeten D (1997) Ethylene and vegetative development. Physiol Plant 100:593–605
  • Wang KLC, Li H, Ecker JR (2002) Ethylene biosynthesis and signaling networks. Plant Cell 14: S131–S151
  • Yang SF, Hoffman NE (1984) Ethylene biosynthesis and its regulation in higher plants. Ann Rev Plant Physiol 35:155–189


Rekord w opracowaniu

Typ dokumentu



Identyfikator YADDA

JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.