An evaluation of dioxin-like polychlorinated biphenyls (DL-PCBs) contamination in sediment from CauBay River was carried out. Ten representative sediment samples were collected in April 2013. The analyzed results indicated the wide occurrence of contamination of DL-PCBs in the CauBay River. In industrial and urban sampling sites, ΣDL-PCB concentrations ranged from 14,254 to 17,369 ng·kg-1 dw, while those in agricultural sampling sites ranged from 9,398 to 13,793 ng·kg-1 dw. The value of (PCB126+PCB169)/ (PCB77+PCB126+PCB169) in CauBay River ranged from 10.9% to 16.1%, suggesting that the DL-PCBs in CauBay river are attributable to both commercial PCBs and pyrogenic sources. The total TEQ value of DLPCBs in the CauBay sediment samples ranged from 5.3 to 11.9 ng TEQ·kg-1 dw. Due to the propensity of DLPCBs to accumulate in various compartments of environment, further evaluation of ecotoxicology should be undertaken as a high priority.