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2011 | 33 | 6 |
Tytuł artykułu

An efficient protocol for particle bombardment-mediated transformation of Centella asiatica callus

Treść / Zawartość
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
In this study, we report an optimization of particle bombardment transformation system for Centella asiatica callus. A total of eight parameters affecting the genetic transformation system were optimized using the synthetic green fluorescent protein (sGFP) as a reporter driven by the CaMV 35S promoter. The results indicated that DNA delivery conditions of 9-cm target distance, 1,100 psi helium pressure, 1.0 µm gold particles size, 27 mmHg chamber vacuum pressure, 2 times number of bombardment, spermidine as precipitation agent, 60 h postbombardment incubation time and 2 µg plasmid DNA concentration were optimal for C. asiatica callus transformation. The expression of sGFP was monitored using fluorescence microscope and further confirmed using RT-PCR. This optimized genetic transformation system is applicable for rapid transient gene analysis and transgenic C. asiatica production.
Słowa kluczowe
Wydawca
-
Rocznik
Tom
33
Numer
6
Opis fizyczny
p.2547-2552,fig.,ref.
Twórcy
autor
  • Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia (UPM), 43400 Serdang, Selangor DE, Malaysia
autor
  • Department of Cell and Molecular Biology, Faculty of Biotechnologyand Biomolecular Sciences, Universiti Putra Malaysia (UPM), 43400 Serdang, Selangor DE, Malaysia
  • Institute of Bioscence, Universiti Putra Malaysia (UPM), 43400 Serdang, Selangor DE, Malaysia
autor
  • Department of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia (UPM), 43400 Serdang, Selangor DE, Malaysia
  • Institute of Bioscence, Universiti Putra Malaysia (UPM), 43400 Serdang, Selangor DE, Malaysia
autor
  • Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia (UPM), 43400 Serdang, Selangor DE, Malaysia
  • Institute of Bioscence, Universiti Putra Malaysia (UPM), 43400 Serdang, Selangor DE, Malaysia
Bibliografia
  • Brinkhaus B, Linder M, Schuppan D, Hahn EG (2000) Chemical, pharmacological and clinical profile of the East Asian medicinal plant Centella asiatica. Phytomedicine 7:427–448
  • Kim OT, Bang KH, Shin YS, Lee MJ, Jung SJ, Hyun DY, Kim YC, Seong NS, Cha SW, Hwang B (2007) Enhanced production of asiaticoside from hairy root cultures of Centella asiatica (L.) Urban elicited by methyl jasmonate. Plant Cell Rep 26: 1941–1949
  • Kim OT, Kim HS, Ohyama K, Muranaka T, Choi YE, Lee HY, Kim MY, Hwang B (2010) Upregulation of phytosterol and triterpene biosynthesis in Centella asiatica hairy roots overexpressed ginseng farnesyl diphosphate synthase. Plant Cell Rep 29: 403–411
  • Krishnan VN, Soni KB, Rajmohan K (2008) Agrobacterium tumefaciens mediated genetic transformation in Centella asiatica L. Urban Curr Biotica 2:1–8
  • Martin KP (2004) Plant regeneration through somatic embryogenesis in medicinally important Centella asiatica L. In Vitro Cell Dev Biol Plant 40:586–591
  • Matsuda H, Morikawa T, Ueda H, Yoshikawa M (2001) Medicinal foodstuffs XXVII: saponin constituents Gotu Kola (2) structures of a new ursane- and oleanane-type triterpene oligoglycosides, centellasaponin B, C, and D, from Centella asiatica cultivated in Sri Lanka. Chem Pharm Bull 49:1368–1371
  • Milen IG, Jost W, Alexandre M (2009) Bioprocessing of plant cell cultures for mass production of targeted compounds. Appl Microbiol Biotechnol 83:809–823
  • Mook-Jung IH, Shin JE, Yun SH, Huh K, Koh JY, Park HK, Jew SS, Jung MW (1999) Protective effects of asiaticoside derivatives against beta-amyloid neurotoxicity. J Neurosci Res 58:417–425
  • Murashige T, Skoog F (1962) A revised medium for rapid growth of and bioassay with tobacco tissue cultures. Physiol Plant 15: 473–497
  • Parveez GKA, Chowdhury MKU, Saleh NM (1997) Physical parameters affecting transient GUS gene expression in oil palm (Elaeis guineensis Jacq.) embryogenic calli via microprojectile bombardment. Ind Crop Prod 8:17–27
  • Patra A, Rai B, Rout GR, Das P (1998) Successful plant regeneration from callus cultures of Centella asiatica (Linn.) Urban. Plant Growth Regul 24:13–16
  • Purkayastha J, Sugla T, Paul A, Solleti SK, Mazumdar P, Basu A, Mohommad A, Ahmed Z, Sahoo L (2010) Efficient in vitro plant regeneration from shoot apices and gene transfer by particle bombardment in Jatropha curcas. Biol Plant 54:13–20
  • Rochange F, Serrano L, Marque C, Teulieres C, Boundet AM (1995) DNA delivery into Eucalyptus globules zygotic embryos through biolistics: optimization of the biological and physical parameters of bombardment for two different particle guns. Plant Cell Rep 14:674–678
  • Southgate EM, Davey MR, Power JB, Marchant R (1995) Factors affecting the genetic engineering of plants by microprojectile bombardment. Biotech Adv 13:631–651
  • Sreeramanan S, Maziah M, Abdullah MP, Sariah M, Xavier M, Nor’Aini MF (2005) Physical and biological parameters affecting transient GUS and GFP expression in banana via particle bombardment. Asia Pac J Mol Biol Biotechnol 13:35–57
  • Suwanaketchanatit C, Piluek J, Peyachoknagul S, Huehne PS (2007) High efficiency of stable genetic transformation in Dendrobium via microprojectile bombardment. Biol Plant 51:720–727
  • Tee CS, Maziah M (2005) Optimization of biolistic bombardment parameters for Dendrobium Sonia 17 calluses using GFP and GUS as the reporter system. Plant Cell Tissue Organ Cult 80: 77–89
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Typ dokumentu
Bibliografia
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Identyfikator YADDA
bwmeta1.element.agro-03d12586-1f8b-4c73-811d-98fa4b386d68
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