Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2013 | 35 | 03 |

Tytuł artykułu

A seed respiration-based index of cold-sensitivity during imbibition in four macrothermal species


Warianty tytułu

Języki publikacji



A research was carried out to evaluate the influence of temperature on seed respiration response of maize, cotton, grain sorghum and sunflower during imbibition, and to define reliable indices for a fast evaluation of cold-sensitivity at germination level in plants. The seed respiration activity was measured during seed imbibition at 25 ºC (optimal) and 15 ºC (suboptimal) constant temperatures, using a homemade respiration chamber adapted to an infrared gas analyzer. At 15 ºC, sunflower and sorghum maintained high levels of seed germination (C90 %), whilst this last dropped in cotton (36.7 %) and maize (27.8 %). With respect to this, cotton and maize seem to be cold sensitive during germination. Instantaneous seed respiration during imbibition versus temperature or thermal time could not be used as a good indicator for cold tolerance, since the levels of CO2 recorded at 15 ºC in cotton (higher than the other species) and maize (similar to that of sorghum and sunflower) did not correspond to adequate seed germination. Differently, the rates (b coefficient of linear regressions) of accumulation of CO2 respired at optimal and suboptimal temperatures during the first hours of imbibition (up to approximately 24 h from the start of experiment), were significantly different in maize and cotton, whilst they did not differ in sorghum and sunflower. Therefore, the shift between slopes may represent a reliable index for seed cold-sensitivity assessment during early germination.

Słowa kluczowe








Opis fizyczny



  • CNR-Istituto per i Sistemi Agricoli e Forestali del Mediterraneo (ISAFoM), Sede di Catania, Str.le V. Lancia, Zona Industriale, Blocco Palma I, 95121 Catania, Italy
  • CNR-Istituto per i Sistemi Agricoli e Forestali del Mediterraneo (ISAFoM), Sede di Catania, Str.le V. Lancia, Zona Industriale, Blocco Palma I, 95121 Catania, Italy


  • Anastasi U, Cammarata M, Abbate V (2000) Yield potential and oil quality of sunflower (oleic and standard) grown between autumn and summer. Ital J Agron 4:23–36
  • Bewley JD (1997) Seed germination and dormancy. Plant Cell 9:1055–1066
  • Bewley JD, Black M (1994) Seeds: physiology of development and germination, 2nd edn. Plenum Press, New York
  • Botha FC, Potgieter GP, Botha AM (1992) Respiratory metabolism and gene expression during seed germination. Plant Growth Regul 11:211–224
  • Brown DM (1975) Heat units for maize in southern Ontario. Ontario Ministry of agriculture and food, Canada. Fact sheet AGDEX 111/31, Order No. 75-077
  • Chapman GW (1985) Measurement of sunflower seed respiration rates and quotients by gas-solid chromatography in a closed system. J Sci Food Agric 36:326–332
  • Chen F, Martin RC, Song S, Nonogaki H (2010) Seed development and germination. In: Trigiano RN, Gray DJ (eds) Plant tissue culture, development and biotechnology. CRS Press, Taylor & Francis Group, Boca Raton, pp 127–140
  • Constable GA, Shaw AJ (1988) Temperature requirements for cotton. Report No. Agfact P5.3.5, NSW Agriculture & Fisheries, Sydney
  • Cross HZ, Zuber MS (1972) Prediction of flowering dates in maize based on different methods of estimating thermal units. Agron J 64:351–355
  • Dahal P, Bradford KJ, Jones RA (1990) Effects of priming and endosperm integrity on seed germination rates of tomato genotypes. I: germination at suboptimal temperature. J Exp Bot 41:1431–1439
  • Dahal P, Kim N, Bradford K (1996) Respiration and germination rates of tomato seeds at suboptimal temperatures and reduced water potentials. J Exp Bot 47:941–947
  • Ellis RA, Roberts EH (1981) The quantification of ageing and survival in orthodox seeds. Seed Sci Technol 9:373–409
  • Foti S, Cosentino SL, Patane` C, D’Agosta G (2002) Effect of osmoconditioning upon seed germination of sorghum (Sorghum bicolor (L.) Moench) under low temperature. Seed Sci Technol 30:521–533
  • Gbur EE, Thomas GL, Miller FR (1979) Use of segmented regression in the determination of the base temperature in heat accumulation models. Agron J 71:949–953
  • Gimeno V, Ferna´ndez-Martı´nez JM, Fereres E (1989) Winter planting as a means of drought escape in sunflower. Field Crop Res 22:307–316
  • Glantz SA, Slinker BK (1990) Primer of applied regression and analysis of variance. McGraw-Hill, New York
  • Go´rnik K, de Castro RD, Liu Y, Bino RJ, Groot SPC (1997) Inhibition of cell division during cabbage (Brassica oleracea L.) seed germination. Seed Sci Res 7:333–340
  • Gresta F, Avola G, Onofri A, Anastasi U, Cristaudo A (2011) When does hard coat impose dormancy in legume seeds? Lotus and Scorpiurus case study. Crop Sci 51:1739–1747
  • Haigh AH, Barlow EWR (1987) Water relations of tomato seeds germination. Aust J Plant Physiol 14:485–492
  • Hegarty TW (1977) Seed activation and seed germination under moisture stress. New Phytol 78:349–359
  • ISTA (1996) The germination test: international rules for seed testing. Seed Sci Technol 24:155–202
  • Khalifa FM, Schneiter AA, Eltayeb EI (2000) Temperature germination responses of sunflower (Helianthus annuus L.) genotypes. Helia 23:97–104
  • Leviatov S, Shoseyov O, Wolf S (1994) Roles of different seed components in controlling tomato seed germination at low temperature. Sci Hort 56:197–206
  • Melethiou-Christou MS, Diamantoglou S, Mitrakos K (1990) Analysis of lipids of Citrullus lanatus (cv sugar baby) during seed germination and seedling growth. J Exp Bot 41:1455–1460
  • Motulsky HJ (2007) Prism 5 statistics guide, GraphPad Software Inc., San Diego. Accessed 17 Oct 2012
  • Patane` C, Gresta F (2006) Germination of Astragalus hamosus and Medicago orbicularis as affected by seed-coat dormancy breaking techniques. J Arid Environ 67:165–173
  • Patane` C, Cavallaro V, Avola G, D’Agosta G (2006) Seed respiration of sorghum (Sorghum bicolor (L.) Moench) during germination as affected by temperature and osmoconditioning. Seed Sci Res 16:251–260
  • Ranal MA, Santana DG, Ferreira WR, Mendes-Rodrigues C (2009) Calculating germination measurements and organizing spreadsheets. Rev Bras Bot 32:849–855
  • Salon C, Raymond P, Pradet A (1988) Quantification of carbon fluxes through the tricarboxylic acid cycle in early germinating lettuce embryos. J Biol Chem 263:12278–12287
  • Woodstock LW (1973) Physiological and biochemical tests for seed vigor. Seed Sci Technol 1:127–157
  • Woodstock LW, Combs MF (1965) Effects of gamma-irradiation of corn seed on the respiration and growth of the seedling. Am J Bot 52:568–569
  • Woodstock LW, Pollock BM (1965) Physiological predetermination: imbibition, respiration and growth of lima bean seeds. Science 150:1031–1032
  • Zhang L, van der Werf W, Zhang S, Li B, Spiertz JHJ (2008) Temperature-mediated developmental delay may limit yield of cotton in relay intercrops with wheat. Field Crop Res 106:258–268


rekord w opracowaniu

Typ dokumentu



Identyfikator YADDA

JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.