Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2012 | 34 | 2 |
Tytuł artykułu

Exogenous cinnamic acid regulates antioxidant enzyme activity and reduces lipid peroxidation in drought-stressed cucumber leaves

Treść / Zawartość
Warianty tytułu
Języki publikacji
To investigate the physiological mechanisms of drought stress mitigated by exogenous cinnamic acid (CA), cucumber seedlings were pretreated with 50 µM CA for 2 days and then were subjected to 10% polyethylene glycol (PEG) 6000. We examined if CA protects plants from PEGinduced drought stress, and whether the protective effect is related to antioxidant and lipid-peroxidation regulation. 2 days of CA application enhanced the activities of guaiacol peroxidase (GPX), glutathione peroxidase (GSH-Px), dehydroascorbate reductase (DHAR) and glutathione reductase (GR) and increased the levels of ascorbate, proline, soluble sugar, vanillic acid (VA) and CA in leaves. After CA-pretreated leaves were exposed to drought, the activities of superoxide dismutase (SOD), catalase, ascorbate peroxidase, monodehydroascorbate reductase, GPX, GSH-Px, DHAR and GR were higher than under drought stress alone, while the levels of reduced glutathione, ascorbate, proline, soluble sugar,VAand CA in leaves were more. The combination of exogenous CA and drought led to higher transcript levels of Cu/Zn-SOD and Mn-SOD genes and decreased contents of malonaldehyde and hydrogen peroxide, but drought had adverse effects on them. Furthermore, the combined effects of exogenous CA and drought made 61.67% leaf edges dried, while drought resulted in 95.83% withered leaves. We conclude CA pretreatment leads to higher contents of CA and VA in drought-stressed leaves and thereby results in higher antioxidant activities directly or indirectly via proline and soluble sugar, thus increasing drought tolerance of cucumber. We also propose soluble sugar can reduce reactive oxygen species and decrease lipid peroxidation when exogenous CA mitigates drought stress.
Słowa kluczowe
Opis fizyczny
  • State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai'an, 271018 Shandong, People's Republic of China
  • State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai'an, 271018 Shandong, People's Republic of China
  • State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai'an, 271018 Shandong, People's Republic of China
  • State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai'an, 271018 Shandong, People's Republic of China
  • State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai'an, 271018 Shandong, People's Republic of China
  • Ahmed CB, Rouina BB, Sensoy S, Boukhriss M, Abdullah FB (2010) Exogenous proline effects on photosynthetic performance and antioxidant defense system of young olive tree. J Agric Food Chem 58:4216–4222
  • Alscher RG, Donahue JL, Cramer CL (1997) Reactive oxygen species and antioxidants: relationships in green cells. Physiol Plant 100:224–233
  • Aravind P, Prasad MNV (2005) Modulation of cadmium-induced oxidative stress in Ceratophyllum demersum by zinc involves ascorbate–glutathione cycle and glutathione metabolism. Plant Physiol Biochem 43:107–116
  • Asada K (1992) Ascorbate peroxidase—a hydrogen peroxide-scavenging enzyme in plants. Physiol Plant 85:235–241
  • Bates LS, Waldren RP, Teare ID (1973) Rapid determination of free proline for water-stress studies. Plant Soil 39:205–207
  • Baziramakenga R, Simard RR, Leroux GD (1994) Effect of benzoic and cinnamic acids on growth, mineral composition, and chlorophyll contents of soybean. J Chem Ecol 20:2821–2833
  • Bernt E, Bergmeyer HU (1974) Inorganic peroxides. In: Bergmeyer HU (ed) Methods of enzymatic analysis. Academic Press, New York, pp 2246–2248
  • Bian S, Jiang Y (2009) Reactive oxygen species, antioxidant enzyme activities and gene expression patterns in leaves and roots of Kentucky bluegrass in response to drought stress and recovery. Sci Hortic 120:264–270
  • Bohnert HJ, Jensen RG (1996) Strategies for engineering water-stress tolerance in plants. Trends Biotechnol 14:89–97
  • Bolouri-Moghaddam MR, Le Roy K, Xiang L, Rolland F, Van den Ende W (2010) Sugar signalling and antioxidant network connections in plant cells. FEBS J 277:2022–2037
  • Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254
  • Chaparzadeh N, D’Amico ML, Khavari-Nejad RA, Izzo R, Navari-Izzo F (2004) Antioxidative responses of Calendula officinalis under salinity conditions. Plant Physiol Biochem 42:695–701
  • Couée I, Sulmon C, Gouesbet G, El Amrani A (2006) Involvement of soluble sugars in reactive oxygen species balance and responses to oxidative stress in plants. J Exp Bot 57:449–459
  • Dhindsa RS, Plumb-Dhindsa P, Thorpe TA (1981) Leaf senescence: correlated with increased levels of membrane permeability and lipid peroxidation, and decreased levels of superoxide dismutase and catalase. J Exp Bot 32:93–101
  • Diaz-Vivancos P, Rubio M, Mesonero V, Periago PM, Ros Barceló A, Martínez-Gómez P, Herna ndez JA (2006) The apoplastic antioxidant system in Prunus: response to long-term plum pox virus infection. J Exp Bot 57:3813–3824
  • Djanaguiraman M, Devi DD, Shanker AK, Sheeba JA, Bangarusamy U (2005) Selenium—an antioxidative protectant in soybean during senescence. Plant Soil 272:77–86
  • Doulis AG, Debian N, Kingston-Smith AH, Foyer CH (1997) Differential localization of antioxidants in maize leaves. Plant Physiol 114:1031–1037
  • El-Basyouni SZ, Chen D, Ibrahim RK, Neish AC, Towers GHN (1964) The biosynthesis of hydroxybenzoic acids in higher plants. Phytochemistry 3:485–492
  • Foyer CH, Descourvieres P, Kunert KJ (1994) Protection against oxygen radicals: an important defence mechanism studied in transgenic plants. Plant Cell Environ 17:507–523
  • Foyer CH, Halliwell B (1976) The presence of glutathione and glutathione reductase in chloroplasts: a proposed role in ascorbic acid metabolism. Planta 133:21–25
  • Foyer CH, López-Delgado H, Dat JF, Scott IM (1997) Hydrogen peroxide and glutathione-associated mechanisms of acclamatory stress tolerance and signaling. Physiol Plant 100:241–254
  • Foyer CH, Noctor G (2000) Oxygen processing in photosynthesis: regulation and signalling. New Phytol 146:359–388
  • Foyer CH, Theodoulou FL, Delrot S (2001) The functions of interand intracellular glutathione transport systems in plants. Trends Plant Sci 6:486–492
  • Fridovich I (1975) Superoxide dismutase. Annu Rev Biochem 44:147–159
  • Ganesan V, Thomas G (2001) Salicylic acid response in rice: influence of salicylic acid on H₂O₂ accumulation and oxidative stress. Plant Sci 160:1095–1106
  • Gao Y, Guo YK, Lin SH, Fang YY, Bai JG (2010) Hydrogen peroxide pretreatment alters the activity of antioxidant enzymes and protects chloroplast ultrastructure in heat-stressed cucumber leaves. Sci Hortic 126:20–26
  • Ghareib HRA, Abdelhamed MS, Ibrahim OH (2010) Antioxidative effects of the acetone fraction and vanillic acid from Chenopodium murale on tomato plants. Weed Biol Manag 10:64–72
  • Goicoechea N, Merino S, Sánchez-Díaz M (2005) Arbuscular mycorrhizal fungi can contribute to maintain antioxidant and carbon metabolism in nodules of Anthyllis cytisoides L. subjected to drought. J Plant Physiol 162:27–35
  • Gong H, Zhu X, Chen K, Wang S, Zhang C (2005) Silicon alleviates oxidative damage of wheat plants in pots under drought. Plant Sci 169:313–321
  • Guo R, Yuan G, Wang Q (2011) Effect of sucrose and mannitol on the accumulation of health-promoting compounds and the activity of metabolic enzymes in broccoli sprouts. Sci Hortic 128:159–165
  • Guri A (1983) Variation in glutathione and ascorbic acid content among selected cultivars of Phaseolus vulgaris prior to and after exposure to ozone. Can J Plant Sci 63:733–737
  • Hayat Q, Hayat S, Irfan M, Ahmad A (2010) Effect of exogenous salicylic acid under changing environment: a review. Environ Exp Bot 68:14–25
  • Hoque MA, Banu MNA, Okuma E, Amako K, Nakamura Y, Shimoishi Y, Murata Y (2007) Exogenous proline and glycinebetaine increase NaCl-induced ascorbate-glutathione cycle enzyme activities, and proline improves salt tolerance more than glycinebetaine in tobacco Bright Yellow-2 suspensioncultured cells. J Plant Physiol 164:1457–1468
  • Hwang SY, Lin HW, Chern RH, Lo HF, Li L (1999) Reduced susceptibility to water logging together with high-light stress is related to increases in superoxide dismutase and catalase activities in sweet potato. Plant Growth Regul 27:167–172
  • Imlay JA (2003) Pathways of oxidative damage. Annu Rev Microbiol 57:395–418
  • Jaleel CA, Gopi R, Manivannan P, Panneerselvam R (2007) Responses of antioxidant defense system of Catharanthus roseus (L.) G. Don. to paclobutrazol treatment under salinity. Acta Physiol Plant 29:205–209
  • Jiménez A, Hernández JA, Pastori G, del Río LA, Sevilla F (1998) The role of the ascorbate-glutathione cycle of mitochondria and peroxisomes in the senescence of pea leaves. Plant Physiol 118:1327–1335
  • Kampfenkel K, Van Montagu M, Inzé D (1995) Extraction and determination of ascorbate and dehydroascorbate from plant tissue. Anal Biochem 225:165–167
  • Khalid KA, Teixeira da Silva JA, Cai W (2010) Water deficit and polyethylene glycol 6000 affects morphological and biochemical characters of Pelargonium odoratissimum (L.). Sci Hortic 125:159–166
  • Khanna-Chopra R, Selote DS (2007) Acclimation to drought stress generates oxidative stress tolerance in drought-resistant than - susceptible wheat cultivar under field conditions. Environ Exp Bot 60:276–283
  • Kumar GNM, Knowles NR (1993) Changes in lipid peroxidation and lipolytic and free-radical scavenging enzyme activities during aging and sprouting of potato (Solanum tuberosum) seed—tubers. Plant Physiol 102:115–124
  • Lee DH, Lee CB (2000) Chilling stress-induced changes of antioxidant enzymes in the leaves of cucumber: in gel enzyme activity assays. Plant Sci 159:75–85
  • Li Q, Yu B, Gao Y, Dai AH, Bai JG (2011) Cinnamic acid pretreatment mitigates chilling stress of cucumber leaves through altering antioxidant enzyme activity. J Plant Physiol 168:927–934
  • Li Y, Lee KK, Walsh S, Smith C, Hadingham S, Sorefan K, Cawley G, Bevan MW (2006) Establishing glucose- and ABA-regulated transcription networks in Arabidopsis by microarray analysis and promoter classification using a Relevance Vector Machine. Genome Res 16:414–427
  • Liang Y, Chen Q, Liu Q, Zhang W, Ding R (2003) Exogenous silicon (Si) increases antioxidant enzyme activity and reduces lipid peroxidation in roots of salt-stressed barley (Hordeum vulgare L.). J Plant Physiol 160:1157–1164
  • Liu JJ, Lin SH, Xu PL, Wang XJ, Bai JG (2009) Effects of exogenous silicon on the activities of antioxidant enzymes and lipid peroxidation in chilling-stressed cucumber leaves. Agric Sci China 8:1075–1086
  • Ludlow MM, Muchow RC (1990) A critical evaluation of traits for improving crop yields in water-limited environments. Adv Agron 43:107–153
  • Luster DG, Donaldson RP (1987) Orientation of electron transport activities in the membrane of intact glyoxysomes isolated from castor bean endosperm. Plant Physiol 85:796–800
  • Manivannan P, Jaleel CA, Kishorekumar A, Sankar B, Somasundaram R, Sridharan R, Panneerselvam R (2007) Changes in antioxidant metabolism of Vigna unguiculata (L.) Walp. By propiconazole under water deficit stress. Colloids Surf B Biointerf 57:69–74
  • Manivannan P, Jaleel CA, Somasundaram R, Panneerselvam R (2008) Osmoregulation and antioxidant metabolism in drought-stressed Helianthus annuus under triadimefon drenching. Comp Rend Biol 331:418–425
  • Mansouri A, Makris DP, Kefalas P (2005) Determination of hydrogen peroxide scavenging activity of cinnamic and benzoic acids employing a highly sensitive peroxyoxalate chemiluminescencebased assay: structure-activity relationships. J Pharm Biomed Anal 39:22–26
  • Mao X, Liu M, Wang X, Liu C, Hou Z, Shi J (2003) Effects of deficit irrigation on yield and water use of greenhouse grown cucumber in the North China Plain. Agric Water Manage 61:219–228
  • Mittler R (2002) Oxidative stress, antioxidants and stress tolerance. Trends Plant Sci 7:405–410
  • Noctor G, Foyer CH (1998) Ascorbate and glutathione: keeping active oxygen under control. Annu Rev Plant Physiol Plant Mol Biol 49:249–279
  • Ohkawa H, Ohishi N, Yagi K (1979) Assay for lipid peroxides in animal tissue by thiobarbituric acid reaction. Anal Biochem 95:351–358
  • Pereira GJG, Molina SMG, Lea PJ, Azevedo RA (2002) Activity of antioxidant enzymes in response to cadmium in Crotalaria juncea. Plant Soil 239:123–132
  • Qiu ZB, Liu X, Tian XJ, Yue M (2008) Effects of CO₂ laser pretreatment on drought stress resistance in wheat. J Photochem Photobiol B Biol 90:17–25
  • Ramel F, Sulmon C, Bogard M, Couée I, Gouesbet G (2009) Differential patterns of reactive oxygen species and antioxidative mechanisms during atrazine injury and sucrose-induced tolerance in Arabidopsis thaliana plantlets. BMC Plant Biol 9:28
  • Ramiro DA, Guerreiro-Filho O, Mazzafera P (2006) Phenol contents, oxidase activities, and the resistance of coffee to the leaf miner Leucoptera coffeella. J Chem Ecol 32:1977–1988
  • Santarius KA (1973) The protective effect of sugars on chloroplast membranes during temperature and water stress and its relationship to frost, desiccation and heat resistance. Planta 113:105–114
  • Schonhof I, Kläring HP, Krumbein A, Clauβben W, Schreiner M (2007) Effect of temperature increase under low radiation conditions on phytochemicals and ascorbic acid in greenhouse grown broccoli. Agric Ecosyst Environ 119:103–111
  • Shi Q, Zhu Z (2008) Effects of exogenous salicylic acid on manganese toxicity, element contents and antioxidative system in cucumber. Environ Exp Bot 63:317–326
  • Shigeoka S, Ishikawa T, Tamoi M, Miyagawa Y, Takeda T, Yabuta Y, Yoshimura K (2002) Regulation and function of ascorbate peroxidase isoenzymes. J Exp Bot 53:1305–1319
  • Ślesak I, Hałdaś W, Ślesak H (2006) Influence of exogenous carbohydrates on superoxide dismutase activity in Trifolium repens L. explants cultured in vitro. Acta Biol Cracov Bot 48:93–98
  • Smirnoff N (1993) The role of active oxygen in the response of plants to water deficit and desiccation. New Phytol 125:27–58
  • Tang K, Zhan JC, Yang HR, Huang WD (2010) Changes of resveratrol and antioxidant enzymes during UV-induced plant defense response in peanut seedlings. J Plant Physiol 167:95–102
  • Türkan I, Bor M, Ozdemir F, Koca H (2005) Differential responses of lipid peroxidation and antioxidants in the leaves of droughttolerant P. acutifolius Gray and drought-sensitive P. vulgaris L. subjected to polyethylene glycol mediated water stress. Plant Sci 168:223–231
  • Vyas D, Kumar S (2005) Purification and partial characterization of a low temperature responsive Mn-SOD from tea (Camellia sinensis (L.) O. Kuntze). Biochem Biophys Res Commun 329:831–838
  • Wang WB, Kim YH, Lee HS, Kim KY, Deng XP, Kwak SS (2009) Analysis of antioxidant enzyme activity during germination of alfalfa under salt and drought stresses. Plant Physiol Biochem 47:570–577
  • Wang Y, Ying Y, Chen J, Wang X (2004) Transgenic Arabidopsis overexpressing Mn-SOD enhanced salt-tolerance. Plant Sci 167:671–677
  • Wei J, Miao H, Wang Q (2011) Effect of glucose on glucosinolates, antioxidants and metabolic enzymes in Brassica sprouts. Sci Hortic 129:535–540
  • Xu PL, Guo YK, Bai JG, Shang L, Wang XJ (2008) Effects of long-term chilling on ultrastructure and antioxidant activity in leaves of two cucumber cultivars under low light. Physiol Plant 132:467–478
  • Xue T, Hartikainen H, Piironen V (2001) Antioxidative and growthpromoting effect of selenium on senescing lettuce. Plant Soil 237:55–61
  • Ye SF, Zhou YH, Sun Y, Zou LY, Yu JQ (2006) Cinnamic acid causes oxidative stress in cucumber roots, and promotes incidence of Fusarium wilt. Environ Exp Bot 56:255–262
  • Yemm EW, Willis AJ (1954) The estimation of carbohydrates in plant extracts by anthrone. Biochem J 57:508–514
  • Yu JQ, Matsui Y (1997) Effects of root exudates of cucumber (Cucumis sativus) and allelochemicals on ion uptake by cucumber seedlings. J Chem Ecol 23:817–827
  • Yu JQ, Matsui Y (1994) Phytotoxic substanees in root exudates of cucumber (Cucumis sativus L). J Chem Ecol 20:21–31
  • Yu JQ, Ye SF, Zhang MF, Hu WH (2003) Effects of root exudates. aqueous root extracts of cucumber (Cucumis sativus L.) and allelochemicals on photosynthesis and antioxidant enzymes in cucumber. Biochem Syst Ecol 31:129–139
  • Yun BW, Huh GH, Kwon SY, Lee HS, Jo JK, Kwak SS (1998) Antioxidant enzymes in Nicotiana cells containing an Ipomoea peroxidase gene. Phytochemistry 48:1287–1290
  • Zhang J, Huang W, Pan Q, Liu Y (2005) Improvement of chilling tolerance and accumulation of heat shock proteins in grape berries (Vitis vinifera cv. Jingxiu) by heat pretreatment. Postharvest Biol Technol 38:80–90
  • Zhang M, Duan L, Tian X, He Z, Li J, Wang B, Li Z (2007) Uniconazole-induced tolerance of soybean to water deficit stress in relation to changes in photosynthesis, hormones and antioxidant system. J Plant Physiol 164:709–717
  • Zhang M, Zhai Z, Tian X, Duan L, Li Z (2008) Brassinolide alleviated the adverse effect of water deficits on photosynthesis and the antioxidant of soybean (Glycine max L.). Plant Growth Regul 56:257–264
  • Zhao H, Yang H (2008) Exogenous polyamines alleviate the lipid peroxidation induced by cadmium chloride stress in Malus hupehensis Rehd. Sci Hortic 116:442–447
  • Zhu Z, Wei G, Li J, Qian Q, Yu J (2004) Silicon alleviates salt stress and increases antioxidant enzymes activity in leaves of saltstressed cucumber (Cucumis sativus L.). Plant Sci 167:527–533
Rekord w opracowaniu
Typ dokumentu
Identyfikator YADDA
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.