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2015 | 61 | 4 |

Tytuł artykułu

Incubation methods for the detection of fungi associated with caraway (Carum carvi L.) seeds

Treść / Zawartość

Warianty tytułu

PL
Inkubacyjne metody wykrywania grzybów na nasionach kminku (Carum carvi L.)

Języki publikacji

EN

Abstrakty

EN
Infestation with fungi may significantly affect the quality of seeds. However, there is no standard method for caraway (Carum carvi L.) seed health testing. The aim of the present study was to determine the most efficient method of the detection of fungi associatyed with caraway seeds. Seven incubation methods for evaluation of health of these seeds were compared: deep freeze blotter test, blotter test with mannitol, blotter test with polyethylene glycol, agar tests on potato-dextrose-agar (PDA) and on reduced PDA (RPDA) without seed disinfection, and agar tests on PDA and RPDA after seed disinfection. The evaluation was performed after 10 and 14 days of incubation. Thirty two fungal genera were associated with the seeds. Alternaria alternata, Cladosporium spp. and Rhizopus nigricans were identified most frequently. Prolongation of incubation time favoured growth of Fusarium spp. and R. nigricans to the highest extent. The greatest seed infestation with fungi, especially Alternaria spp., was observed in the deep freeze blotter test followed by the blotter test with mannitol. Both of them could be recommended for further study on caraway seed health testing.
PL
Zasiedlenie przez grzyby może istotnie wpływać na jakość nasion, jednak nie ma obecnie standardowej metody oceny zdrowotności nasion kminku (Carum carvi L). Celem prezentowanych badań było określenie optymalnej metody wykrywania grzybów zasiedlających nasiona kminku. Porównywano siedem metod oceny zdrowotności tych nasion: test bibułowy z przemrażaniem nasion, test bibułowy z mannitolem, test bibułowy z glikolem polietylenowym, test agarowy na pożywce glukozowo-ziemniaczanej (PDA) i zubożonej pożywce PDA (RPDA) bez odkażania nasion i test agarowy na pożywce PDA i RPDA po odkażaniu nasion. Ocenę przeprowadzono po 10 i 14 dniach inkubacji. Na nasionach stwierdzono występowanie 32 rodzajów grzybów. Najczęściej identyfikowano Alternaria alternata, Cladosporium spp. i Rhizopus nigricans. Wydłużenie inkubacji w największym stopniu sprzyjało wzrostowi Fusarium spp. i R. nigricans. Największe zasiedlenie nasion przez grzyby, zwłaszcza Alternaria spp., obserwowano w teście bibułowym z przemrażaniem nasion, a następnie w teście bibułowym z mannitolem i oba te testy można polecić do dalszych badań nad oceną zdrowotności nasion kminku.

Słowa kluczowe

Wydawca

-

Czasopismo

Rocznik

Tom

61

Numer

4

Opis fizyczny

p.9-22,ref.

Twórcy

autor
  • Department of Plant Pathology, Seed Science and Technology, Division of Seed Science and Technology, Faculty of Horticulture and Landscape Architecture, Poznan University of Life Sciences, Szamotulska 28, Baranowo, 62-081 Przezmierowo, Poland
autor
  • Department of Plant Pathology, Seed Science and Technology, Division of Seed Science and Technology, Faculty of Horticulture and Landscape Architecture, Poznan University of Life Sciences, Szamotulska 28, Baranowo, 62-081 Przezmierowo, Poland
autor
  • Department of Plant Pathology, Seed Science and Technology, Division of Seed Science and Technology, Faculty of Horticulture and Landscape Architecture, Poznan University of Life Sciences, Szamotulska 28, Baranowo, 62-081 Przezmierowo, Poland

Bibliografia

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Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

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