The aim of the study conducted in 2012-2014 was to evaluate multi-breeding value of the tomato male-sterile lines (gene ms-10). Two (PWS 10, PWS 18) of the five tested lines showed the highest and most stable level of sterility. The good stability of sterility was also found in the two other ms-10 lines (PWS 12, PWS 21), which displayed a low number of fruits with seeds. In the case of the last tested line (PWS 19), regardless of the research year, partially fertile plants were observed. A large variation among tested lines was demonstrated in terms of most analysed agrobotanical traits, as well as the content of basic chemical components and health-enhancing constituents.