Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2011 | 33 | 2 |

Tytuł artykułu

Determination of ploidy and homozygosity of carrot plants obtained from anther cultures

Warianty tytułu

Języki publikacji



The purpose of the present work was to evaluate carrot plants obtained from anther cultures with respect to their ploidy and homozygosity. Ploidy was determined using flow cytometry. Homozygosity of analyzed plants was determined using isoenzymes, glucose-6-phosphate isomerase (PGI) and aspartate aminotransferase (AAT). The cytometric tests revealed that more than 90% of the carrot plants obtained from anther cultures were doubled haploid. In the initial assessment of polymorphism of the two enzymatic systems in selected androgenetic carrot plants of the cultivars: Berjo, Kazan F₁, and Splendid F₁, it was proven that 100% of those plants were homozygotes in respect to PGI and also with respect to AAT. In the second experiment, the obtained androgenetic progeny of the heterozygous donor plant of cultivar Narbonne F₁ was found to be 94% homozygotic with respect to PGI and 100% homozygotic in the case of AAT. For the androgenetic plants of the cultivar Kazan F₁, 89% of them were homozygotic with respect to AAT but PGI enzyme system did not differentiate the homozygotic and heterozygotic androgenetic progeny. These results indicated that enzyme polymorphism depends on carrot genotype, therefore, analysis of some (more than one) isozymes was necessary to confirm homozygosity of plants. PGI and AAT can be a useful tool for determining homozygosity in androgenetic carrot plants.

Słowa kluczowe








Opis fizyczny



  • · Research Institute of Vegetable Crops, Konstytucji 3Maja 1/3, 96-100 Skierniewice, Poland
  • Research Institute of Vegetable Crops, Konstytucji 3Maja 1/3, 96-100 Skierniewice, Poland
  • Kutno Sugar Beet Breeding Company, Straszkow, 62-650 Klodawa, Poland
  • Research Institute of Vegetable Crops, Konstytucji 3Maja 1/3, 96-100 Skierniewice, Poland
  • Institute of Plant Genetic, Polish Academy of Science, Strzeszynska 34, 60-479 Poznan, Poland
  • Research Institute of Vegetable Crops, Konstytucji 3Maja 1/3, 96-100 Skierniewice, Poland


  • Andersen SB, Christiansen J, Farestveit B (1990) Carrot (Daucus carota L.): In vitro production of haploids and field trials. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 12. Springer, Berlin, pp 393–402
  • Bartošová Z, Obert B, Takáč T, Kormuťák A, Preťová A (2005) Using enzyme polymorphism to identify the gametic origin of flax regenerants. Acta Biol Crac Ser Botanica 47(1):173–178
  • Cardoso MB, Kaltchuk-Santos E, de Mundstock EC, Bodanese-Zanettini MH (2004) Initial segmentation patterns of microspores and pollen viability in soybean cultured anthers: indication of chromosome doubling. Braz Arch Biol Technol 47(5):703–712
  • Chen CC, Kasha KJ, Marsolais A (1984) Segmentation patterns and mechanisms of genome multiplication in cultured microspores of barley. Can J Genet Cytol 26:475–483
  • de Pentado OMI, Garcia P, de la Perez Vega MP (1997) Isozyme markers and genetic variability in three species of Centrosema (Leguminosae). Braz J Genet 20(3):443–452
  • Ferrie AMR (2007) Doubled haploid production in nutraceutical species: a review. Euphytica 158:347–357
  • Funasaka T, Yanagawa T, Hogan V, Raz A (2005) Regulation of phosphoglucose isomerase/autocrine motility factor expression by hypoxia. FASEB J 19:1422–1430
  • Galbraith DW (1984) Flow cytometric analysis of the cell cycle. Cell Cult Som Cell Genet Plants 1:765–777
  • Górecka K, Krzyżanowska D, Górecki R (2005a) The influence of several factors on the efficiency of androgenesis in carrot. J Appl Genet 46(3):265–269
  • Górecka K, Krzyżanowska D, Kiszczak W, Górecki R (2005b) Embryo induction in anther culture of Daucus carota L. Veget Crops Res Bull 63:25–32
  • Górecka K, Krzyżanowska D, Kiszczak W, Kowalska U (2009a) Carrot doubled haploids. In: Touraev A, Forster BP, Mohan Jain S (eds) Advances in haploid production in higher plants. Springer, New York, pp 231–239
  • Górecka K, Krzyżanowska D, Kiszczak W, Kowalska U (2009b) Plant regeneration from carrot (Daucus carota L.) anther culture derived embryos. Physiol Plant 31:1139–1145
  • Gottlieb LD (1973) Enzyme differentiation and phylogeny in Clarkia franciscana, C. rubicunda and C. amoena. Evolution 27:205–214
  • Höfer M, Touraev A, Heberle-Bors E (1999) Induction of embryogenesis from isolated apple microspores. Plant Cell Rep 18:1012–1017
  • Hu KL, Matsubara S, Murakami K (1993) Haploid plant production by anther culture in carrot (Daucus carota L.). J Japan Soc Hort Sci 62(3):561–565
  • Matsubara S, Dohya N, Murakami K, Nishio T, Dore C (1995) Callus formation and regeneration of adventitious embryos from carrot, fennel and mitsuba microspores by anther and isolated microspore cultures. Acta Hort 392:129–137
  • Selander RK, Smith MH, Yang SY, Johnson WE, Gentry JB (1971) Biochemical polymorphism and systematics in the genus Peromyscus. I. Variation in the old-field mouse (Peromyscus polionotus). Univ Tex Publ 7103:49–90
  • Silvente S, Camas A, Lara M (2003) Molecular cloning of the cDNA encoding aspartate aminotransferase from bean root nodules and determination of its role in nodule nitrogen metabolism. J Exp Bot 54(387):1545–1551
  • Smykalová I, Šmirous P Jr, Kubošiová A, Gasmanová N, Griga M (2005) Dihaploid production via anther culture in Czech breeding lines of caraway (Carum carvi L.). Abstracts XII International Conference on Plant Embryology, September 5–7 2005 Cracow, Poland. Acta Biol Crac Ser Botanica 47(1):52
  • Song W (2001) Identification of different maize (Zea mays L.) genotypes using isozyme and RAPD marker analysis. Master dissertation, Institute of Plant Genetics, Polish Academy of Sciences, Poznań, Poland
  • Sunderland N, Collins GB, Dunwell JM (1974) The role of nuclear fusion in pollen embryogenesis of Datura innoxia Mill. Planta 117:227–241
  • Szklarczyk M (1996) Isoenzymatic markers. In: Michalik B (ed) The use of biotechnological methods in plant breeding. Drukpol, Kraków, pp 90–98 (in polish)
  • Testillano P, Georgiev S, Mogensen HL, Coronado MJ, Dumas C, Risueno MC, Matthys-Rochon E (2004) Spontaneous chromosome doubling results from nuclear fusion during in vitro maize induced microspore embryogenesis. Chromosoma 112:342–349
  • Tyukavin GB, Shmykova NA, Mankhova MA (1999) Cytological study of embryogenesis in cultured carrot anthers. Russ J Plant Physiol 46(6):876–884
  • Weeden FN, Gottlieb LD (1980) Isolation of cytoplasmic enzymes from pollen. Plant Phys 66:400–403
  • Westphal L, Wricke G (1989) Genetic analysis of DIA, GOT and PGI isozyme loci in Daucus carota L. ssp. sativus. Plant Breed 102:51–57
  • Zeidler M (2000) Electrophoretic analysis of plant isozymes. Act Univ Palacki Olomun Fac Rer Nat Biol 38:7–16


rekord w opracowaniu

Typ dokumentu



Identyfikator YADDA

JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.