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2013 | 35 | 05 |

Tytuł artykułu

Physical stress for overproduction of biomass and flavonoids in cell suspension cultures of Boesenbergia rotunda

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Flavonoid, a bioactive compound isolated from the rhizomes of Boesenbergia rotunda, exhibited antibiotic and anti-inflammatory properties and has also shown high inhibitory activity of dengue-2 virus protease. Several factors are responsible for the production of flavonoid in cell cultures. In the present study, the effects of initial inoculation volume, temperature and speed of agitation on cell growth, total and selected flavonoid in suspension cultures of B. rotunda were determined. High performance liquid chromatography analysis showed that a 2 % inoculation volume induced a significantly high accumulation of biomass and of flavonoid in the cells. The cells cultured at 25 ºC showed significantly high biomass and selected flavonoid accumulation while differences in medium agitation significantly affected the yield of selected flavonoid.

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Opis fizyczny



  • Centre of Biotechnology for Agriculture Research, Biotechnology and Bioproduct Research Cluster, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia
  • Faculty of Plantation and Agrotechnology, University of Technology MARA (UiTM), 40450 Shah Alam, Selangor, Malaysia 123
  • Centre of Biotechnology for Agriculture Research, Biotechnology and Bioproduct Research Cluster, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia
  • Centre of Biotechnology for Agriculture Research, Biotechnology and Bioproduct Research Cluster, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia


  • Baque MA, Moh SH, Lee EJ, Zhong JJ, Paek KY (2012) Production of biomass and useful compounds from adventitious roots of high-value added medicinal plants using bioreactor. Biotechnol Adv. doi:10.1016/j.biotechadv.2011.11.004
  • Chan LK, Koay SS, Boey PL, Bhatt A (2010) Effects of abiotic stress on biomass and anthocyanin production in cell cultures of Melastoma malabathricum. Biol Res 43:127–135
  • Chattopadhyay S, Srivastava AK, Bhojwani SS, Bisaria VS (2002) Production of podophyllotoxin by plant cell cultures of Podophyllum hexandrum in bioreactor. J Biosci Bioeng 93:215–220. doi:10.1016/s1389-1723(02)80017-2
  • Contin A, van der Heijden R, ten Hoopen HJG, Verpoorte R (1998) The inoculum size triggers tryptamine or secologanin biosynthesis in a Catharanthus roseus cell culture. Plant Sci 139:205–211. doi:10.1016/s0168-9452(98)00188-5
  • Fahey JW, Stephenson KK (2002) Pinostrobin from honey and Thai ginger (Boesenbergia pandurata): a potent flavonoid inducer of mammalian phase 2 chemoprotective and antioxidant enzymes. J Agric Food Chem 50:7472–7476
  • Jaipetch T, Kanghae S, Pancharoen O, Patrick V, Reutrakul V, Tuntiwachwuttikul P, White A (1982) Constituents of Boesenbergia pandurata: isolation, crystal structure and synthesis of (±)-boesenbergin A. Aust J Chem 35:351–361
  • Jeong CS, Murthy HN, Hahn EJ, Lee HL, Paek KY (2009) Inoculum size and auxin concentration influence the growth of adventitious roots and accumulation of ginsenosides in suspension cultures of ginseng (Panax ginseng C.A. Meyer). Acta Physiol Plant 31:219–222
  • Kiat TS, Pippen R, Yusof R, Ibrahim H, Khalid N, Rahman NA (2006) Inhibitory activity of cyclohexenyl chalcone derivatives and flavonoids of fingerroot, Boesenbergia rotunda (L.), towards dengue-2 virus NS3 protease. Bioorg Med Chem Lett 16:3337–3340
  • Kirana C, Jones G, Record I, McIntosh G (2007) Anticancer properties of panduratin A isolated from Boesenbergia pandurata; (Zingiberaceae). J Nat Med 61:131–137. doi:10.1007/s11418-006-0100-0
  • Larsen K (1996) A preliminary checklist of the Zingiberaceae of Thailand. Thai For Bull (Bot) 24:35–49
  • Le Bail JC, Aubourg L, Habrioux G (2000) Effects of pinostrobin on estrogen metabolism and estrogen receptor transactivation. Cancer Lett 156:37–44
  • Morris P (1986) Regulation of product synthesis in cell cultures of Catharanthus roseus. Effect of culture temperature. Plant Cell Rep 5:427–429. doi:10.1007/bf00269633
  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant 15: 473–497
  • Pandji C, Grimm C, Wray V, Witte L, Proksch P (1993) Insecticidal constituents from four species of the Zingiberaceae. Phytochemistry 34:415–419
  • Shim J-S, Han Y-S, Hwang J-K (2009) The effect of 4-hydroxypanduratin A on the mitogen-activated protein kinase-dependent activation of matrix metalloproteinase-1 expression in human skin fibroblasts. J Dermatol Sci 53:129–134. doi:10.1016/j.jdermsci.2008.09.002
  • Shin GH, Chio MG, Lee DW (2003) Comparative study of the effects of various culture conditions on cell growth and gagaminine synthesis in suspension culture of Cynanchum wilfordii (Maxim.) Hemsley. Biol Pharm Bull 26:1321–1325
  • Srivastava P, Sisodia V, Chaturvedi R (2011) Effect of culture conditions on synthesis of triterpenoids in suspension cultures of Lantana camara L. Bioproc Biosys Eng 34:75–80
  • Tewtrakul S, Subhadhirasakul S, Puripattanavong J, Panphadung T (2003) HIV-1 protease inhibitory substances from the rhizomes of Boesenbergia pandurata Holtt. Songklanakarin J Sci Technol 25:503–508
  • Trakoontivakorn G, Nakahara K, Shinmoto H, Takenaka M, Onishi-Kameyama M, Ono H, Yoshida M, Nagata T, Tsushida T (2001) Structural analysis of a novel antimutagenic compound, 4-hydroxypanduratin A, and the antimutagenic activity of flavonoids in a Thai spice, fingerroot (Boesenbergia pandurata Schult.) against mutagenic heterocyclic amines. J Agric Food Chem 49:3046–3050
  • Wu D, Nair MG, DeWitt DL (2002) Novel compounds from Piper methysticum Forst (kava kava) roots and their effect on cyclooxygenase enzyme. J Agric Food Chem 50:701–705
  • Zhang W, Seki M, Furusaki S (1997) Effect of temperature and its shift on growth and anthocyanin production in suspension cultures of strawberry cells. Plant Sci 127:207–214. doi:10.1016/s0168-9452(97)00124-6
  • Zhang W, Curtin C, Franco C (2002) Towards manipulation of postbiosynthetic events in secondary metabolism of plant cell cultures. Enzyme Microb Technol 30:688–696
  • Zhao J, Verpoorte R (2007) Manipulating indole alkaloid production by Catharanthus roseus cell cultures in bioreactors: from biochemical processing to metabolic engineering. Phytochem Rev 6:435–457. doi:10.1007/s11101-006-9050-0
  • Zhong JJ, Yoshida T (1993) Effects of temperature on cell growth and anthocyanin production in suspension cultures of Perilla frutescens. J Ferment Bioeng 76:530–531


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