PL EN


Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2014 | 12 | 2 |
Tytuł artykułu

Increased production of carrageenase by Pseudomonas aeruginosa ZSL-2 using Taguchi experimental design

Treść / Zawartość
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
The culture conditions for the production of carrageenase were optimized using one-factor-at-a-time method combined with orthogonal array design. With the one-factor-at-a-time method revealed optimal conditions for carrageenase production were 24 h of fermentation period, 28 °C incubation temperature at pH 8.0 with NaNO3 as nitrogen source and carrageenan as carbon source in MMS media. Further optimization of carrgeenase production by using orthogonal experimental design L9 (34) with four factors, temperature, pH, NH4NO3 and carrageenan with their relevant levels revealed optimised conditions for carrageenase production were temperature of 28 °C, pH 8.0, 2 g L-1 NaNO3 and 2 g L-1 carrageenan. The order of the factors affecting the fermentation process was found to be temperature > pH > NaNO3 > carrageenan. The temperature played a significant role on the carrageenase production. Higher carrageenase yield with activity of 0.542 ±0.045 U ml-1 was obtained in the optimised medium when compared to those of basal medium. Carrageenase hydrolysed products of carrageenan were identified by LC-ESI-MS as neocarrabiose, neocarrabiose-4 sulfate, neocarratetraose, neocarratetraose-4 sulfate, anhydrogalactose, galactose, galactose-4 sulphate and sulphate.
Wydawca
-
Rocznik
Tom
12
Numer
2
Opis fizyczny
p.194-207,fig.,ref.
Twórcy
autor
  • Department of Biochemistry, Gulbarga University, Gulbarga - 585106, Karnataka, India
autor
  • Department of Biochemistry, Gulbarga University, Gulbarga - 585106, Karnataka, India
autor
  • Department of Studies and Research in Biochemistry, Tumkur University, Tumkur - 572103, Karnataka, India
Bibliografia
  • [1] Michel G., Nyval-Collen P., Barbeyron T., Czjzek M., Helbert W., Appl Microbiol Biotechnol. 71 (2006) 23-33.
  • [2] Knutsen S., Myslabodski D., Larsen B., Usov A., Bot Mar. 37 (1994) 163-169.
  • [3] Guzman-Maldonado H., Paredes-Lopez O., Critical Review in Food Science Nutrition 35 (1995) 373-403.
  • [4] Beldman G, Mutter M, Searle-van Leeuwen MJF, van den Broek LAM, Schols HA, Voragen AGJ. Visser J, & Voragen, A.G.J. Amsterdam: Elsevier; 1996.
  • [5] Weigl J., Yaphe W., Can J of Microbiol. 12 (1966) 939-947.
  • [6] Le Gall Y., Braud J. P., Kloareg B., Plant Cell Rep 8 (1990) 582-585.
  • [7] Ostgaard K., Wangen B. F., Knutsen S. H., Aasen I. M., Enzy Microbial Technol. 5 (1993) 326-333.
  • [8] Mou H., Jiang X., Guan H., J of Appl Phycol. 15( 2003) 297-303.
  • [9] Alban S., Schauerte A., Franz G., Carbohydrte Polymer 47 (2002) 267-276.
  • [10] Arfors K. E., Ley K., J Lab Clinical Medicine 121 (1993) 201-202.
  • [11] Suzuki N, Kitazato K, Takamatsu J, Saito H. Thrombosis and Haemostasis. 65 (1991)369-373.
  • [12] Hiroishi S, Sugie K, Yoshida T, Morimoto J, Taniguchi Y, Imai S, Kurebayashi J. Cancer Letters. 167 (2001) 167:145-150.
  • [13] Caceres P. J., Carlucci M. J., Damonte E. B., Matsuhiro B., Zuniga E. A., Phytochemistry 53 (2000) 81-86.
  • [14] Knutsen S., Myslabodski D., Larsen B., Usov A Bot Mar 37 (1994) 163-169.
  • [15] Araki T., Higashimoto Y., Morishita T., Fisheries Science. 65 (1999) 937-942.
  • [16] Greasham R. L., Biotechnology. In: Rehm HJ, Read G, Puhler A, Stagler P (Eds.), Bioprocessing, vol. 3. VCH Publishers, Inc., New York; 1983.
  • [17] Thomas D. M., J of Chem Engi. 6 (1977) 180.
  • [18] Deming S. N., Morgan SL., Elsevier, Oxford; 1987.
  • [19] Ziayoddin M., Shinde M., Lalitha J., The Bioscan. 4 (2012) 096-095.
  • [20] Ziayoddin M., Manohar S, Lalitha J., The Bioscan. 5 (2010) 279-283.
  • [21] Dygert S., Li L. H., Florida D., Thoma J. A., Anal Biochem. 13 (1965) 367-374.
  • [22] Lowry O. H., Rosebrough N. J., Farr A.L., Randall R. J., J of Biol Chem. 193 (1951) 265-275.
  • [23] Basawaraj A. K., Manohar S., Lalitha J. Biotechnology and Bioprocess Engineering 18 (2013) 333-341.
  • [24] Khambhaty Y, Mody K, Jha B., Biotechnol Bioproc Engi. 12 (2007) 668-675.
  • [25] Sarwar G., Matayoshi S., Oda H., Microbiol Immunol. 31 (1987) 869-877.
  • [26] Potin P., Sanseau A., Le Gall Y., Rochas C., Kloareg B., Europ J of Biochem. 201 (1991) 241-247.
  • [27] Mou H. J., Jiang X. L., Jiang X., Guan H. S., J Fish Sci China 9 (2002) 251-254.
  • [28] Yaphe W., Baxter B., Appl Microbiol 3 (1955) 380-383
  • [29] Sarwar G., Sakata T., Kakimoto D., Bulletin of the Japanese Society of Scientific Fisheries 49 (1983) 1689-1694.
  • [30] Mao-hong Zhou, Jian-she Ma, Jun Li, Hai-ren Ye, Ke-xin Huang, Xiao-wei Zhao, Biotechnol Bioproc Engi 13 (2008) 545-551.
  • [31] McLean M. M., Williamson F. B., Europ J of Biochem. 93 (1979) 553-558.
  • [32] Lakshmikanth M., Manohar S., Patnakar J., Vaishampayan P., Shouche Y., Lalitha J., World J of Microbiol and Biotechnol. 22 (2006) 531-537.
  • [33] Agbo J., Moss M., Canadian J. Microbiol. 115 (1979) 355-368.
  • [34] Li Y., Chen J., Lun S. Y., Rui X. S., Appl Microbiol and Biotechnol. 55 (2001) 680-685.
  • [35] Tarng Y. S., Juang S. C., Chang C. H., J of Materials Processing Technology 128 (2002) 1-6.
  • [36] Krishna P. K., Venkata M. S., Sreenivas R. R., Bikas R. P., Sarma P. N., Biochem Engi J. 24 (2005) 17-26.
  • [37] Sreenivas R. R., Prakasham R. S., Krishna P. K., Rajesham S., Sarma P. N., Venkateswar R. L., Proc Biochem. 39 (2004) 951-956.
  • [38] Antonopoulos A., Favetta P., Helbert W., Lafosse M., Anal Chem. 77 (2005) 4125-4136.
  • [39] Aristotelis A., Hardouin J., Favetta P., Helbert W., Lafosse M., Rapid Communications In Mass Spectrometry 19 (2005) 2217-2226.
Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.agro-03067c46-0387-4490-bb5a-414f277e45b7
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.