Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2013 | 35 | 10 |

Tytuł artykułu

Needle terpene concentrations and emissions of two coexisting subspecies of Scots pine attacked by the pine processionary moth (Thaumetopoea pityocampa)

Warianty tytułu

Języki publikacji



Mediterranean pine forests are often attacked by caterpillars of Thaumetopoea pityocampa (Lep., Thaumetopoidae), one of the most important defoliators in the Mediterranean region causing large economic losses and ecological effects. The needle terpene concentrations and emissions may play a key role in the defense of pines. We studied two subspecies of Pinus sylvestris, nevadensis (an endemic and relict subspecies) and iberica, with different levels of caterpillar attack in Sierra Nevada mountains (Spain). GC–MS analyses showed large total concentrations of terpenes (6 to 39 mg g-1 of dry weight) in the needles of both subspecies under field conditions. Concentrations were 25 % higher in ‘‘Non-Attacked Trees’’ (NATs) of the iberica than in the nevadensis subspecies. The branches of NATs had terpene concentrations 20 % higher than those of ‘‘Attacked Branches of attacked trees’’ (ABs). Within attacked trees, the ‘‘Non-Attacked Branches’’ (NABs) also had terpene concentrations 20 % higher than those of ABs. Mainly a-pinene and germacrene D had higher concentrations in NATs and NABs than in ABs. Some terpenes had higher concentrations in NABs than in NATs, indicating possible systemic reactions. In subsp. nevadensis, the percentage of monoterpenes relative to total terpenes was higher in ABs than in other attack states. The rates of emission in nevadensis (standardized to 30 C) were ca. three times higher in ABs than in NABs and NATs. These results suggest that the lower terpene concentrations and high percentages of monoterpenes in ABs were produced by a combination of emission losses and terpene induction in response to herbivorous attack.

Słowa kluczowe








Opis fizyczny



  • Needle terpene concentrations and emissions of two coexisting subspecies of Scots pine attacked by the pine processionary moth (Thaumetopoea pityocampa)
  • Needle terpene concentrations and emissions of two coexisting subspecies of Scots pine attacked by the pine processionary moth (Thaumetopoea pityocampa)
  • Grupo de Ecologı´a Terrestre, Departamento de Biologı´a Animal y Ecologı´a, Facultad de Ciencias, Universidad de Granada, 18071 Granada, Spain
  • Needle terpene concentrations and emissions of two coexisting subspecies of Scots pine attacked by the pine processionary moth (Thaumetopoea pityocampa)


  • Battisti A, Stastny M, Netherer S, Robinet C, Schopf A, Roques A, Larsson S (2005) Expansion of geographic range in the pine processionary moth caused by increased winter temperatures. Ecol Appl 15:2084–2096
  • Bauce E, Crepin M, Carisey N (1994) Spruce budworm growth, development and food utilization on young and old Balsam Fir trees. Oecologia 97:499–507
  • Beyaert I, Kopke D, Stiller J, Hammerbacher A, Yoneya K, Schmidt A, Gershenzon J, Hilker M (2012) Can insect egg deposition ‘warn’ a plant of future feeding damage by herbivorous larvae? Proc R Soc B 279:101–108
  • Blanch JS, Penuelas J, Llusia J (2007) Sensitivity of terpene emissions to drought and fertilization in terpene-storing Pinus halepensis and non-storing Quercus ilex. Physiol Plant 131:211–225
  • Blanch JS, Llusia J, Niinemets U, Noe SM, Penuelas J (2011) Instantaneous and historical temperature effects on alpha-pinene Llusia J, Penuelas J, Seco R, Filella I (2012) Seasonal changes in the
  • daily emission rates of terpenes by Quercus ilex and the atmospheric concentrations of terpenes in the natural park of Montseny, NE Spain. J Atmos Chem 69:215–230
  • Martin DM, Gershenzon J, Bohlmann J (2003) Induction of volatile terpene biosynthesis and diurnal emission by methyl jasmonate in foliage of Norway spruce. Plant Physiol 132:1586–1599
  • Mumm R, Hilker M (2006) Direct and indirect chemical defence of pine against folivorous insects. Trends Plant Sci 11:351–358
  • Nykanen H, Koricheva J (2004) Damage-induced changes in woody plants and their effects on insect herbivore performance: a metaanalysis. Oikos 104:247–268
  • Ormeno E, Fernandez C, Mevy J-P (2007) Plant coexistence alters terpene emission and content of Mediterranean species. Phytochemistry 68:840–852
  • Paiva MR, Mateus E, Santos MH, Branco MR (2011) Pine volatiles mediate host selection for oviposition by Thaumetopoea pityocampa (Lep., Notodontidae). J Appl Entomol 135:195–203
  • Paris CI, Llusia J, Penuelas J (2010) Changes in monoterpene emission rates of Quercus ilex infested by aphids tended by native or invasive Lasius ant species. J Chem Ecol 36:689–698
  • Pasqua G, Monacelli B, Manfredini C, Loreto F, Perez G (2002) The role of isoprenoid accumulation and oxidation in sealing wounded needles of Mediterranean pines. Plant Sci 163:355–359
  • Penuelas J, Llusia J (2004) Plant VOC emissions: making use of the unavoidable. Trends Ecol Evol 19:402–404
  • Penuelas J, Staudt M (2010) BVOCs and global change. Trends Plant Sci 15:133–144
  • Petrakis PV, Roussis V, Papadimitriou D, Vagias C, Tsitsimpikou C (2005) The effect of terpenoid extracts from 15 pine species on the feeding behavioural sequence of the late instars of the pine processionary caterpillar Thaumetopoea pityocampa. Behav Process 69:303–322
  • Phillips MA, Croteau RB (1999) Resin-based defenses in conifers. Trends Plant Sci 4:184–190
  • Sampedro L, Moreira X, Llusia J, Penuelas J, Zas R (2010) Genetics, phosphorus availability, and herbivore-derived induction as sources of phenotypic variation of leaf volatile terpenes in a pine species. J Exp Bot 61:4437–4447
  • Sampedro L, Moreira X, Zas R (2011) Costs of constitutive and herbivore-induced chemical defences in pine trees emerge only under low nutrient availability. J Ecol 99:818–827
  • Staudt M, Lhoutellier L (2007) Volatile organic compound emission from hohn oak infested by gypsy moth larvae: evidence for distinct responses in damaged and undamaged leaves. Tree Physiol 27:1433–1440
  • Steele CL, Crock J, Bohlmann J, Croteau R (1998) Sesquiterpene synthases from grand fir (Abies grandis)—Comparison of constitutive and wound-induced activities, and cDNA isolation, characterization and bacterial expression of delta-selinene synthase and gamma-humulene synthase. J Biol Chem 273: 2078–2089
  • Su JW, Zeng JP, Qin XW, Ge F (2009) Effect of needle damage on the release rate of Masson pine (Pinus massoniana) volatiles. J Plant Res 122:193–200
  • Tiberi R, Niccoli A, Curini M, Epifano F, Marcotullio MC, Rosati O (1999) The role of the monoterpene composition in Pinus spp. needles, in host selection by the pine processionary caterpillar, Thaumetopoea pityocampa. Phytoparasitica 27:263–272
  • Trapp S, Croteau R (2001) Defensive resin biosynthesis in conifers. Annu Rev Plant Physio 52:689–724
  • Vet LEM, Dicke M (1992) Ecology of infochemical use by natural enemies in a tritrophic context. Annu Rev Entomol 37:141–172
  • Wainhouse D, Staley JT, Jinks R, Morgan G (2009) Growth and defence in young pine and spruce and the expression of resistance to a stem-feeding weevil. Oecologia 158:641–650
  • Yassaa N, Williams J (2007) Enantiomeric monoterpene emissions from natural and damaged Scots pine in a boreal coniferous forest measured using solid-phase microextraction and gas chromatography/mass spectrometry. J Chromatogr A 1141:138–144
  • Zhang QH, Schlyter F, Battisti A, Birgersson G, Anderson P (2003) Electrophysiological responses of Thaumetopoea pityocampa females to host volatiles: implications for host selection of active and inactive terpenes. Anz Schadl J Pest Sc 76:103–107
  • Zovi D, Stastny M, Battisti A, Larsson S (2008) Ecological costs on local adaptation of an insect herbivore imposed by host plants and enemies. Ecology 89:1388–1398


rekord w opracowaniu

Typ dokumentu



Identyfikator YADDA

JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.