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2012 | 34 | 1 |

Tytuł artykułu

Morpho-histological characterization of truncated leaf syndrome seedlings: an oil palm (E. guineensis Jacq.) somaclonal variant

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A comparative phenotypic and morpho-histological study was carried out on tissue culture-derived truncated leaf syndrome (TLS) and wild-type oil palm seedlings to investigate their phenotypic and morpho-histological differences. On the basis of the percentage of TLS occurence in a clone, the TLS seedlings were categorized into three groups: severe (70–100%), moderate (40–69%) and mild (\40%). Wild and TLS seedlings differ in terms of growth, vigor, leaf size and shape, root number, volume, length as well as the size of shoot apical meristem (SAM). Differences were also found in fresh weight of leaf, root and SAM of TLS in comparison to wild-type seedlings. Depressed and wavy leaf surface, sunken and distorted stomata and coalesced epidermal cells were observed by scanning electron microscopy in TLS seedlings. The size, shape and number of stomata were also different in the TLS leaf compared to the wild type. Longer epidermal cells, depressed epidermal layer, larger sub-epidermal cells and loosely arranged less mesophyll cells were observed in TLS leaf than in wild type. Undifferentiated vascular bundle was found in TLS leaves where metaxylem and phloem were absent and root tips were impaired. The size and leaf primordial arrangement of SAM were remarkably different in TLS compared to wild-type seedlings suggesting that these alterations might be due to smaller SAM. Therefore, further detailed genetic analysis on TLS SAM is needed for clear understanding of TLS occurrence.

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  • Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia
  • Felda Biotechnology Centre, PT 23417, Lengkuk Technology, Persiaran Technolohy, 71760 Bandar Enstek, Negeri Sembilan, Malaysia
  • Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia
  • Institute of Tropical Agriculture, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia
  • Advanced Biotechnology and Breeding Centre, Malaysian Palm Oil Board, No.6, Persiaran Instituti, Bandar Baru Bangi, 43000 Kajang, Selangor, Malaysia
  • Department of Crop Science, Faculty of Agriculture, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia
  • Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia
  • Institute of Tropical Agriculture, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia


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