Global brain ischemia in Mongolian gerbils: Assessing the level of anastomosis in the cerebral circle of Willis

• Autorzy: Subiros Martinez N. , Machado J. M. , Perez Saad H. , Coro Antich R. M. , Berlanga Acosta J. A. , Rodriguez Salgueiro S. , Garcia Illera G. , Suarez Alba J. , Garcia del Barco Herrera D.
• Języki publikacji: EN

Abstrakty

EN

The Mongolian gerbil has been widely used as a global brain ischemia model because of its incomplete cerebral circle of Willis. However, the inter-individual anatomic variability of this vascular structure interferes with the reliability of the model. The aim of this work was to introduce modifications to the protocol of global brain ischemia experiments in Mongolian gerbils in an attempt to increase the reliability and usefulness of this model. Our study focused on the assessment of the level of anastomosis of the cerebral circle of Willis in order to evaluate its contribution to clinicopathological outcomes in this model. Sham-operated, Ischemic, and Ischemic + Hypothermia animals were subjected to a 15-minute occlusion of the common carotid arteries. Transcardiac perfusion with bromophenol blue / gelatin solution was performed 72 hours after ischemia. Brains were processed for anatomopathological analysis. Tissue damage was observed in the hippocampus, caudate-putamen nucleus, neocortex, and thalamic nuclei of animals from the Ischemic group. The circles of Willis of the Sham-operated animals showed bilateral (38%), unilateral (48%) or no posterior communicating arteries (14%). A negative correlation between infarct volume and the level of anastomosis was revealed for the Ischemic, but not for the Ischemic + Hypothermia group. Additionally, Analysis of covariance (ANCOVA) was performed to assess the contribution of the level of anastomosis to the clinicopathological outcomes. It was confirmed that the infarct volume decreased in the Ischemic + Hypothermia group when compared to the Ischemic group. Since the level of anastomosis cannot be predicted, this variable should necessarily be considered when analyzing the results of global brain ischemia in Mongolian gerbils.

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2012
• Tom: 72
• Numer: 4
• Opis fizyczny: p.377-384,fig.,ref.

Twórcy

autor

Subiros Martinez N.

• Department of Pharmaceutics, Center for Genetic Engineering and Biotechnology, La Habana, Cuba

autor

Machado J. M.

• Department of Pharmaceutics, Center for Genetic Engineering and Biotechnology, La Habana, Cuba

autor

Perez Saad H.

• Department of Experimental Neurology, Institute of Neurology and Neurosurgery, La Habana, Cuba

autor

Coro Antich R. M.

• National Center for Medical Information (INFOMED), La Habana, Cuba

autor

Berlanga Acosta J. A.

• Department of Pharmaceutics, Center for Genetic Engineering and Biotechnology, La Habana, Cuba

autor

Rodriguez Salgueiro S.

• Electron Microscopy Laboratory, National Center for Scientific Research (CNIC), La Habana, Cuba

autor

Garcia Illera G.

• Department of Pharmaceutics, Center for Genetic Engineering and Biotechnology, La Habana, Cuba

autor

Suarez Alba J.

• Department of Pharmaceutics, Center for Genetic Engineering and Biotechnology, La Habana, Cuba

autor

Garcia del Barco Herrera D.

• Department of Pharmaceutics, Center for Genetic Engineering and Biotechnology, La Habana, Cuba

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