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1991 | 42 | 3 |
Tytuł artykułu

Enhancement of the physicochemical qualities of gastric mucus by sofalcone

Treść / Zawartość
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
The effect of prolonged administration of an antiulcer drug, sofalcone, on the physicochemical properties of gastric mucus was investigated. The experiments were conducted with groups of rats receiving twice daily for three consecutive days a dose of 100 mg/kg sofalcone, while the control group received daily doses of vehicle. The rats were sacrificed 16 h after the last dose and gastric mucosa subjected to physicochemical measurements. The results revealed that sofalcone evoked a 23% increase in mucus gel dimension, while sulfo- and sialomucins content of the gel increased by 54 and 25%, respectively. These changes were accompanied by a 16% increase in mucus H⁺ retardation capacity, 2-fold increase in viscosity, and a 39% increase in the gel hydrophobicity. The mucus elaborated in the presence of sofalcone contained 67 % more covalently bound fatty acids, exhibited 10% lower content of protein, 30% higher content of carbohydrate, and 18% higher content of lipids. The mucus of the sofalcone group also showed an increase in the proportion of the high molecular weight mucus glycoprotein form, which in the control group accounted for about 30% of gel mucin, while its content in mucus gel of animals receiving sofalcone reached the value of 50%. The results indicate that sofalcone enhances the protective qualities of mucus component of gastric mucosal barrier.
Wydawca
-
Rocznik
Tom
42
Numer
3
Opis fizyczny
p.293-304,fig.,ref.
Twórcy
  • Research Center, University of Medicine and Dentistry of New Jersey, Newark, New Jersey 07103-2400, USA
autor
  • Research Center, University of Medicine and Dentistry of New Jersey, Newark, New Jersey 07103-2400, USA
autor
  • Research Center, University of Medicine and Dentistry of New Jersey, Newark, New Jersey 07103-2400, USA
autor
  • Research Center, University of Medicine and Dentistry of New Jersey, Newark, New Jersey 07103-2400, USA
  • Research Center, University of Medicine and Dentistry of New Jersey, Newark, New Jersey 07103-2400, USA
Bibliografia
  • 1. Allen A. Structure and function of gastrointestinal mucus. In: Johnson LR, ed. Physiology of the gastrointestinal tract. New York: Baven Press. 1981; 617-639.
  • 2. Slomiany BL, Sarosiek J, Slomiany A. Gastric mucus and the mucosal harrier. Pig Dis 1987; 6: 125-145.
  • 3. Slomiany BL, Murty VLN, Piotrowski J, Slomiany A. Effect of antiulcer agents on the physicochemical properties of gastric mucus. In: Chan tier E, Ratcliffe NA, eds. Mucus and Related Topics. Cambridge: Company of Biologists Ltd. 1989; 179- 191.
  • 4. Flemstrom G, Garner A. Gastroduodenal HCO₃ transport: characteristics and proposed role in activity regulation and mucosal protection. Am J Physiol 1982; 242: G183-G193.
  • 5. Slomiany BL, Piasek A, Sarosiek J, Slomiany A. The role of surface and intracellular mucus in gastric mucosal protection against hydrogen ion. Scand J Gastroenterol 1985; 20: 1191-1196.
  • 6. Murty VLN, Sarosiek J, Slomiany A, Slomiany BL. Effect of lipids and proteins on the viscosity of gastric mucus glycoprotein. Biochem Biophys Bes Commun 1984; 121: 521-529.
  • 7. Yuonan F, Pearson J, Allen A, Venables C. Changes in the structure of the mucous gel on the mucosal surface of the stomach in association with peptic disease. Gastroenterology 1982; 82: 817-831.
  • 8. Bilski J, Murty VLN, Nadziejko C, Aono M, Moriga M, Slomiany A, Slomiany BL. Protection against alcohol-induced gastric mucosal injury by geranylgeranylacetone: effect of indomethacin. Digestion 1988; 41: 22-33.
  • 9. Saziki R, Arai I, Isobe Y, Hirose H, Aihara H. Effect of sofalcone on necrotizing agent-induced gastric lesions and on endogeneous prostaglandins in rat stomach. J Pharm Dyn 1984; 7: 791-797.
  • 10. Fujimoto N, Murakami S, Suwa T, Mori Y. The studies on the role of gastric glycoproteins with reference to cytoprotection: protective effect of prostaglandin Ea and sofalcone on ethanol-inuced necrosis. J Pharm Dyn 1984; 7: 864-871.
  • 11. Slomiany BL, Liau YH, Carter SR, Tsukada H, Slomiany A. Enzymatic sulfation of mucin in gastric mucosa: effects of sofalcone, sucralfate and aspirin. Digestion 1987; 38: 178-186.
  • 12. Sarosiek J, Bilski J, Tsukada H, Slomiany A, Slomiany BL. Effect of solon on gastric mucus viscosity, permeability to hydrogen ion, and susceptibility to pepsin. Digestion 1987;37:238-246.
  • 13. Kerss S, Allen A, Garner A. A single method for measuring thickness of the mucus gel layer adherent to rat, frog and human gastric mucosa: influence of feeding, prostaglandin, N-acetylcystine and other agents. Clin Sci 1982; 63: 187-195.
  • 14. Bilski J, Murty VLN, Nadziejko C, Aono M, Moriga M, Slomiany A, Slomiany BL. Enhanement of the lipid content and physical properties of gastric mucus by geranylgeranylacetone. Biochem Pharmac 1987; 36: 4059-4065.
  • 15. Piotrowski J, Bilski J, Nishikawa H, Slomiany A, Slomiany BL. Enhancement in gastric mucus gel qualities with colloidal bismuth subcitrate administration. Eur J Pharmac 1990; 184: 55-63.
  • 16. Gwozdzinski K, Nishikawa H, Okazaki K, Slomiany A, Slomiany BL. Gastric mucin hydrophobicity: Effects of associated and covalently bound lipids, proteolysis and reduction. Biochem Internatl 1988; 17: 907-917.
  • 17. Lowry OH, Tinsley IJ. A simple sensitive method for lipid phosphorus. Lipids 1974; 9: 491-492.
  • 18. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with Folin phenol reagent. J Biol Chem 1951; 193: 265-275.
  • 19. Slomiany BL, Liau YH, Mizuta K, Slomiany A. Fatty acid acylation of mucin by gastric mucosa: effects of sofalcone and sucralfate. Biochem Pharmac 1987; 36: 3273-3276.
  • 20. Slomiany BL, Nishikawa H, Piotrowski J, Okazaki K, Slomiany A. Lipolytic activity of Campylobacter pylori: effect of sofalcone. Digestion 1989; 43: 33-40.
  • 21. Konturek SJ, Radecki T, Brzozowski T, Drozdowicz D, Piastucki I, Muramatsu M, Tanaka M, Aihara H. Antiulcer and gastroprotective effects of solan, a synthetio flavonoid derivative of sophoradin: role of endogenous prostaglandins. Eur J Pharmac 1986; 125: 185-192.
  • 22. Slomiany BL, Nadziejko C, Mizuta K, Slomiany A. Role of associated and covalently bound lipids in the physicochemical properties of mucus glycoprotein. In: Quinton P, Mastella G, eds. Cellular and molecular basis of cystic fibrosis. San Francisco Press. 1988; 263-279.
  • 23. Slomiany BL, Piotrowski J, Okazaki K, Grzelinska E, Slomiany A. Nature of the enhancement of the protective qualities of gastric mucus by sucralfate. Digestion 1989; 44: 222-231.
  • 24. Slomiany A, Yano S, Slomiany BL, Glass GBJ. Lipid composition of the gastric mucous barrier in the rat. J Biol Chem 1978; 253: 3785-3791.
  • 25. Lichtenberger LM, Graziani LA, Dial EJ, Butler BD, Hills BA. Role of surface active phospholipids in gastric cytoprotection. Science 1983; 219: 1327-1329.
  • 26. Hills BA. Gastric mucosal barrier: stabilization of hydrophilic lining to the stomach by mucus. Am J Physiol 1985; 249: G342-G349.
  • 27. Ka YC, Lichtenberger LM. Localization of phospholipid-rich zones in rat gastric mucosa: possible origin of a protective hydrophobic luminal lining. J Histochem Cytochem 1987; 35: 1285-1291.
  • 28. Ehrlich J, Piotrowski J, Slomiany A, Slomiany BL. Dynamic laser light scattering studies on gastric mucin in health and disease. Gastroenterology 1990;. 98: 40A.
Typ dokumentu
Bibliografia
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Identyfikator YADDA
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