PL EN


Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2008 | 30 | 5 |

Tytuł artykułu

Mitochondrial RNA editing of F0-ATPase subunit 9 gene (atp9) transcripts of Yunnan purple rice cytoplasmic male sterile line and its maintainer line

Autorzy

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
On the base of construction of a new type of cytoplasmic male sterile (CMS) line ZidaoA, we analyzed the editing of transcripts of mitochondrial ATP synthase subunit 9 gene (atp9) from CMS line and its maintainer line. With PCR, RT-PCR, and direct sequencing, complete nucleotide sequences were determined for the mitochondrial atp9 gene and its cDNA from two lines of purple rice type rice: CMS line Ying xiang A and its maintainer line Ying xiang B. The atp9 transcript of Ying xiang A was shown to have no editing sites and the transcript of Ying xiang B was shown to have two editing sites with changes affecting the amino acid sequence of the protein product. The editing of the atp9 transcript from Ying xiang B was found to change an arginine codon into a termination codon, shortening the protein of Ying xiang B to the ‘‘standard’’ size. And the Ying xiang A transcript, which has no termination codon, cannot be translated to a normal protein. The results demonstrate the important role of RNA editing in the production of the functional ATP9 subunit and suggest that RNA editing could be likely associated with cytoplasmic male sterility.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

30

Numer

5

Opis fizyczny

p.657-662.fig.,ref.

Twórcy

autor
  • Key Laboratory of MOE for Plant Development Biology, College of Life Sciences, Wuhan University, 430072 Wuhan, Hubei, People's Republic of China
autor
  • Key Laboratory of MOE for Plant Development Biology, College of Life Sciences, Wuhan University, 430072 Wuhan, Hubei, People's Republic of China
autor
  • Key Laboratory of MOE for Plant Development Biology, College of Life Sciences, Wuhan University, 430072 Wuhan, Hubei, People's Republic of China

Bibliografia

  • Araya A, Domec C, Be´gu D, Litvak S (1992) An in vitro system for the editing of ATP synthase subunit 9 mRNA using wheat mitochondrial extracts. Proc Natl Acad Sci USA 89:1040–1044
  • Bégu D, Graves PV, Domec C, Arselin G, Litvak S, Araya A (1990) RNA editing of wheat mitochondrial ATP synthase subunit 9: direct protein and cDNA sequencing. Plant Cell 2:1283–1290
  • Chapdelaine Y, Bonen L (1991) The wheat mitochondrial gene for subunit I of the NADH dehydrogenase complex: a f/ww-splicing model for this gene-in-pieces. Cell 65:465–472
  • Dewey RE, Levings CS III, Timothy DH (1986) Novel recombinations in the maize mitochondrial genome produce a unique transcriptional unit in the Texas male sterile cytoplasm. Cell 44:439–449
  • Hanson MR (1991) Plant mitochondrial mutations and male sterility. Annu Rev Genet 25:461–486
  • Hanson MR, Bentolila S (2004) Interactions of mitochondrial and nuclear genes that affect male gametophyte development. Plant Cell 16:S154–S169
  • Hernould M, Suharsono S, Litvak S, Araya A, Mouras A (1993) Male sterility induction in transgenic tobacco plants with an unedited ATP9 mitochondrial gene from wheat. Proc Natl Acad Sci USA 90:2370–2374
  • Hernould M, Suharsono S, Zabaleta E, Carde JP, Litvak S, Araya A, Mouras A (1998) Impairment of tapetum and mitochondria in engineered male-sterile tobacco plants. PlantMol Biol 36:499–508
  • Hu Y, Wei L, Liu S, Yu J, Ding Y (2005a) Analysis of male sterilityrelated protein of young panicle in rice (Oryza sativa L.) by twodimensional electrophoresis.Wuhan Univ J Nat Sci 10(3):597–601
  • Hu Y, Wu Q, Liu S, Wei L, Chen X, Yan Z, Yu J, Zeng L, Ding Y (2005b) Study of rice pollen grains by multispectral imaging microscopy. Microsc Res Techn 68(6):335–346
  • Ishikawa M, Kadowaki KI (1993) Excess RNA editing in Rice mitochondrial atp9 transcripts. Plant Cell Physiol 34(6):959–963
  • Kaleikau EK, Andre CP, Walbot V (1990) Sequence of the F0-ATPase proteolipid (atp9) gene from rice mitochondria. Nucleic Acids Res 18:370
  • Kaleikau EK, Andre CP, Walbot V (1993) Transcription of the gene coding for subunit 9 of ATP synthase in rice mitochondria. Plant Mol Biol 22:899–905
  • Kim DS, Cho DS, Park WM, Na HJ, Nam HG (2006) Proteomic pattern-based analyses of light responses in Arabidopsis thaliana wild-type and photoreceptor mutants. Proteomics 6:3040–3049
  • Larbi JG, Stephanie KB, Christine DC (2002) Mitochondrial RNA editing truncates a chimeric open reading frame associated with S male-sterility in maize. Curr Genet 42:179–184
  • Levings CS III (1990) The Texas cytoplasm of maize: cytoplasmic male sterility and disease susceptibility. Science 250:942–947
  • Mignouna H, Vermin SS, Briquet M (1987) Mitochondrial DNA modification associated with cytoplasmic male sterility in rice. Theor Appl Genet 74:666–669
  • Notsu Y, Masood S, Nishikawa T, Kubo N, Akiduki G, Nakazono M, Hirai A, Kadowaki K (2002) The complete sequence of the rice (Oryza sativa L.) mitochondrial genome: frequent DNA sequence acquisition and loss during the evolution of flowering plants. Mol Genet Genomics 268(4):434–445
  • Plomion C, Lalanne C, Claverol S, Meddour H, Kohler A, Bogeat-Triboulot MB, Barre A, Provost G, Dumazet H, Jacob D, Bastien C, Dreyer E, Daruvar A, Guehl JM, Schmitter JM, Martin F, Bonneu M (2006) Mapping the proteome of poplar and application to the discovery of drought-stress responsive proteins. Proteomics 6:6509–6527
  • Rakwal R, Agrawal GK (2003) Rice proteomics: current status and future perspectives. Electrophoresis 24:3378–3389
  • Salazar RA, Pring DR, Kempken F (1991) Editing of mitochondrial atp9 transcripts from two sorghum lines. Curr Genet 20:483–486
  • Schuster W, Brennicke A (1991) RNA editing in ATPase subunit 6 mRNAs in Oenothera mitochondria. FEBS Lett 295:97–101
  • Tanaka N, Mitsui S, Nobori H, Yanagi K, Komatsu S (2005) Expression and function of proteins during development of the basal region in rice seedlings. Mol Cell Proteomics 4:796–808
  • Tian X, Zheng J, Hu S, Yu J (2006) The rice mitochondrial genomes and their variations. Plant Physiol 142(2):401–410
  • Wang Z, Zou Y, Li X, Zhang Q, Chen L, Wu H, Su D, Chen Y, Guo J, Luo D, Long Y, Zhong Y, Liu Y (2006) Cytoplasmic male sterility of rice with Boro II cytoplasm is caused by a cytotoxic peptide and is restored by two related PPR motif genes via distinct modes of mRNA silencing. Plant Cell 18:676–687
  • Wei L, Ding Y, Liu Y, Yu J (2002a) Microcalorimetric analysis of anthers of male sterile rice sterile line. J Wuhan Bot Res 20(4):308–310
  • Wei L, Ding Y, Hu Y, Yu J (2002b) Analysis of leaf proteins of Zidao male sterile line by 2D-PAGE. Acta Genet Sin 29(8):696–699
  • Willson TA, Nagley P (1987) Amino acids substitution in subunit 9 of the mitochondrial ATPase complex of Saccharomyces cerevisiae. Sequence analysis of a series of revertants of an olil mitmutant carrying an amino acid substitution in the hydrophilic loop of subunit 9. Eur J Biochem 167(2):291–297
  • Wintz H, Hanson MR (1991) A termination codon is created by RNA editing in the petunia mitochondrial atp9 gene transcript. Curr Genet 19:61–64
  • Zabaleta E, Mouras A, Hernould M, Suharsono S, Araya A (1996) Transgenic male-sterile plant induced by an unedited atp9 gene is restored to fertility by inhibiting its expression with antisense RNA. Proc Natl Acad Sci USA 93:11259–11263

Uwagi

Rekord w opracowaniu

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

bwmeta1.element.agro-02a47da3-3557-45f1-982f-18e50cb00551
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.