Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2013 | 22 | 4 |

Tytuł artykułu

Phytoindication of the ecogenotoxic effects of vehicle emissions using pollen abortion test with native flora

Warianty tytułu

Języki publikacji



This study aimed to evaluate the rate of ecogenotoxicity caused by traffic emissions at sites in the vicinity of roads and at sites near planned highway construction using a pollen grain abortion assay with higher wild plant species. The relationship between pollen grain abortivity and distance from a road also was assessed. The highest values were found at a distance of 30 m, where the prevailing wind direction was parallel to the road. Wind blowing away from the road shifted this boundary to a distance of 350 m. The results showed the highest genotoxicity at the “Ring road” site, where the frequency of abortive pollen grains was 4.05 times higher than at the control site, and at the “Dubná Skala” site, with induction factor 3.48. Based on our results we can conclude that Chelidonium majus, Cichorium intybus, and Melilotus albus are suitable species for the detection of genotoxicity in the environment.








Opis fizyczny



  • Institute of High Mountain Biology, University of Zilina, Tatranska Javorina 7, SK-059 56, Slovakia
  • Department of Botany, Comenius University in Bratislava, Faculty of Natural Sciences, Revova 39, Bratislava 1, Slovakia


  • 1. MICIETA K., MURiN G. Microspore analysis for genotox­icity of a polluted environment. Environ. Exp. Bot. 36, (1), 21, 1996.
  • 2. MONARCA S., RIZZONI M., GUSTAVINO B., ZANI C., ALBERTI A., FERETTI D., ZERBINI I. Genotoxicity of Surface Water Treated With Different Disinfectants Using In Situ Plant Tests. Environ. Mol. Mutagen. 41, 353, 2003.
  • 3. CARVALHO-OLIVEIRA R., POZO R.M.K., LOBO D.J.A., LICHTENFELS A.J.F.C., MARTINS-JUNIOR H.A., BUSTILHO J.O.W.V., SAIKI M., SATO I.M., SAL- DIVA P.H.N. Diesel emissions significantly influence com­position and mutagenicity of ambient particles: a case study in Sao Paulo, Brazil. Environ. Res. 98, 1, 2005.
  • 4. SOLENSKA M., MICIETA K., MIStK M. Plant bioassays for an in situ monitoring of air near an industrial area and a municipal solid waste - Zilina (Slovakia). Environ. Monit. Assess. 115, 499, 2006.
  • 5. MIStK M., MICIETA K., SOLENSKA M., MIStKOVA K., PISARCtKOVA H., KNASMULLER S. In situ biomoni­toring of the genotoxic effects of mixed industrial emissions using the Tradescantia micronucleus and pollen abortion tests with wild life plants: Demonstration of the efficacy of emission controls in an eastern European city. Environ. Pollut. 145, 459, 2007.
  • 6. CALZONI G.L., ANTOGNONI F., PARI E., FONTI P., GNES A., SPERANZA A. Active biomonitoring of heavy metal pollution using Rosa rugosa plants. Environ. Pollut. doi: 10.1016/j.envpol.2006.12.023. 2007.
  • 7. WULTSCH G., MIStK M., NERSESYAN A., KNASMULLER S. Genotoxic effects of occupational expo­sure measured in lymphocytes of waste-incinerator workers. Mutat. Res. 720, 3, 2011.
  • 8. SPERANZA A., LEOPOLD K., MAIER M., TADDEI A.R., SCOCCIANTI V. Pd-nanoparticles cause increased toxicity to kiwifruit pollen compared to soluble Pd (II). Environ. Pollut. 158, (3), 873, 2010.
  • 9. Ministry of Environment of the Slovak Republic, Slovak Hydrometeorological Institute. Report on air quality and the contribution of each source to the pollution in the Slovak Republic. kvalite_ovzdusia_SR_2008.pdf, 2008 [In Slovak].
  • 10. KNASMULLER S., GOTTMANN E., STEINKELLNER H., FOMIN A., PICKL CH., PASCHKE A., GOD R., KUNDI M. Detection of genotoxic effects of heavy metal contaminated soils with plant bioassays. Mutat. Res. 420, 37, 1998.
  • 11. FARGASOVA A., LISTIAKOVA J. Cr and Ni simultane­ous phytotoxicity and mutagenicity assay. Nova Biotechnologica. 9, 107, 2009.
  • 12. MICIETA K. Bioindication mutagenic effects of polluted environment by higher plants. Zivot. Prostr. 24, (5), 267, 1990 [In Slovak].
  • 13. GRANT W.F. Chromosome Aberrations in Plants as a Monitoring System. Environ. Health Persp. 27, 37, 1978.
  • 14. GRANT W.F. Higher-Plant Assays for the Detection of Genotoxicity in Air Polluted Envirnoments. Ecosystem Health. 4, (4), 210, 1998.
  • 15. NILAN R.A. Potential of Plant Genetic Systems for Monitoring and Screening Mutagens. Environ. Health Persp. 27, 181, 1978.
  • 16. MIStK M., SOLENSKA M., MICIETA K., MIStKOVA K., KNASMULLER S. In situ monitoring of clastogenicity of ambient air in Bratislava, Slovakia using the Tradescantia micronucleus assay and pollen abortion assays. Mutat. Res. 605, 1, 2006.
  • 17. MURtN A. Flowers as indicators of mutagenicity and phy- totoxicity of polluted environments. Biologia (Bratislava). 42, 447, 1987 [In Slovak].
  • 18. GARREC J-G. Use of Pollen in Plant Biomonitoring of Air Pollution. Resource document. EnviroNewsArchives. html, [Cited on 11th October 2009] 2006.
  • 19. MULCAHY D.L. Pollen Tetrads in the Detection of Environmental Mutagenesis. Environ. Health. Persp. 37, 91, 1981.
  • 20. CARNEIRO M.F.H., RIBEIRO F.Q., FERNANDES- FILHO F.N., LOBO D.J.A., BARBOSA Jr F., RHODEN C.R., MAUAD T., SALDIVA P.H.N., CARVALHO- OLIVEIRA R. Pollen abortion rates, nitrogen dioxide by passive diffusive tubes and bioaccumulation in tree barks are effective in the characterization of air pollution. Environ. Exp. Bot. 72, 272, 2011.
  • 21. MICIETA K., MURÍN G. Wild plant species in bio-indica­tion of radioactive-contaminated sites around Jaslovské Bohunice nuclear power plant in the Slovak Republic. JEnviron. Radioativ. 93, 26, 2007.
  • 22. MICIETA K., MURÍN G. Three species of genus Pinus suit­able as bioindicators of polluted environment. Water Air Soil Poll. 104, 413, 1998.
  • 23. Ministry of Environment of the Slovak Republic, Regional Environmental Office in Zilina, Slovak Hydrometeorological Institute. Program to improve air qual­ity in the area of air quality management - City of Martin and Vrútky. HU pdfUH, 2009 [In Slovak].
  • 24. STANOVÁ V., VALACHOVIC M. (Eds.) Catalogue of Slovakian Habitats. DAPHNE - Institute of Applied Ecology, Bratislava: pp. 225, 2002.
  • 25. MEIRELES J., ROCHA R., COSTA NETO A., CERQUEIRA E. Genotoxic effects of vehicle traffic pollu­tion as evaluated by micronuclei test in tradescantia (Trad- MCN). Mutat. Res. 675, 46, 2009.
  • 26. CARRERAS H.A., PIGNATA M.L., SALDIVA P.H.N. In situ monitoring of urban air in Córdoba, Argentina using the Tradescantia-micronucleus (Trad-MCN) bioassay. Atmos. Environ. 40, 7824, 2006.
  • 27. VILLARINI M., FATIGONI C., DOMONICI L., MAESTRI S., EDERLI L., PASQUALINI S., MONARCA S., MORETTI M. Assessing the genotoxicity of urban air pollutants using two in situ plant bioassays. Environ. Pollut. 157, 3354, 2009.
  • 28. KLUMPP A., ANSEL W., KLUMPP G., CALATAYUD V., GARREC J.P., HE S., PENUELAS J., RIBAS A., RO - POULSEN H., RASMUSSEN S., JOSE SANZ M., VERGNE P. Tradescantia micronucleus test indicates geno­toxic potential of traffic emissions in European cities. Environ. Pollut. 139, 515, 2006.
  • 29. KUHNS H., ETYEMEZIAN V., LANDWEHR D., MAC- DOUGALL C., PITCHFORD M., GREEN M. Testing Re - entrained Aerosol Kinetic Emissions from Roads (TRAK- ER): a new approach to infer silt loading on roadways. Atmos. Environ. 35, 2815, 2001.
  • 30. KUHNS H., ETYEMEZIAN V., GREEN M., HENDRICK­SON K., McGOWN M., BARTON K., PITCHFORD M. Vehicle-based road dust emission measurement - Part II: Effect of precipitation, wintertime road sanding, and street sweepers on inferred PM10 emission potentials from paved and unpaved roads. Atmos. Environ. 37, 4573, 2003.
  • 31. CHAN T.L., NING Z., LEUNG C.W., CHEUNG C.S., HUNG W.T., DONG G. On road remote sensing of petrol vehicle emissions measurement and emission factors esti­mation in Hong Kong. Atmos. Environ. 38, 2055, 2004.
  • 32. HITCHINS J., MORAWSKA L., WOLFF R., GILBERT D. Concentrations of submicrometre particles from vehicle emissions near a major road. Atmos. Environ. 34, 51, 2000.
  • 33. SAVOIA E.J.L., DOMINGOS M., GUIMARAES E.T., BRUMATI F., SALDIVA P.H.N. Biomonitoring genotoxic risks under the urban weather conditions and polluted atmosphere in Santo Andre, SP, Brazil, through Trad-MCN bioassay. Ecotox. Environ. Safe. 72, 255, 2009.
  • 34. KLUMPP A., ANSEL W., FOMIN A., SCHNIRRING S., PICKL CH. Influence of climatic conditions on the muta­tions in pollen mother cells of Tradescantia clone 4430 and implications for the Trad-MCN bioassay protocol. Hereditas. 141, 142, 2004.
  • 35. ETYEMEZIAN V., KUHNS H., GILLIES J., CHOW J., HENDRICKSON K., McGOWN M., PITCHFORD M. Vehicle-based road dust emission measurement (III): effect of speed, traffic volume, location and season on PM10 road dust emissions in the Treasure Valley, ID. Atmos. Environ. 37, 4583, 2003.

Typ dokumentu



Identyfikator YADDA

JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.