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2012 | 59 | 2 |
Tytuł artykułu

Myocardial remodeling in rats with metabolic syndrome: role of Rho-kinase mediated insulin resistance

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Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
 Insulin resistance (IR) plays a critical role in metabolic syndrome (MS). Previous studies have demonstrated that activated ROCK is increased in MS patients. However, the effect of Rho-kinase (ROCK) on IR has not been definitely determined. Thus, the aims of the present study were to determine whether ROCK activation induces IR or affects myocardial structure and function, as well as the possible mechanisms underlying this process. Wistar rats fed high fat, high glucose and high salt diet sewed as model of MS and we used transmission electron microscopy, echocardiogram technology, and terminal deoxynucleotidyl transferase-mediated DNA nick-end labeling staining to identify any myocardial damage. The protein levels of MYPT-1 (characteristic of ROCK activation), IRS-1 and AKT were analyzed by immunohistochemistry and Western blotting. In hearts from MS rats, we found increased protein levels of phospho-MYPT-1 and phospho-IRS-1 (Ser307) and decreased phospho-AKT compared to levels in normal rats. In conclusion, the results suggest that ROCK-mediated IR is involved in the development of myocardial impairments in MS rats and that this effect is mediated probably via the IRS-1/PI3-kinase/AKT pathway.
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Rocznik
Tom
59
Numer
2
Opis fizyczny
p.249-254,fig.,ref.
Twórcy
autor
  • Department of Emergency, Shandong University Qilu Hospital, Jinan, Shandong China
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Bibliografia
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