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2017 | 35 | 3 |
Tytuł artykułu

Parameters of variability and diversity of tetrameric STRs for practical use in Zlotnicka White and Zlotnicka Spotted pigs

Treść / Zawartość
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
As a goal of this paper, the assessment of genetic variability of Złotnicka White (ZW) and Zlotnicka Spotted (ZS), was chosen in order to verify the appropriateness of 11 tetrameric Short Tandem Repeats (STRs) panel for use in genetic resources of pigs. Analyses were carried out in sets of 91 ZW and 250 ZS pigs. Seventy-one alleles in ZW and 85 alleles in ZS were detected at all 11 STRs loci. An average number of alleles at locus (MNA) was 6.455 in ZW and 7.727 in ZS. An average number of effective alleles (MNe) was 3.532 in ZW and 3.431 in ZS. Observed heterozygosity Ho was 0.659 in ZW and 0.637 in ZS. On average, polymorphism information content (PIC) reached 0.639 and 0.619 per locus in ZW and ZS. The probability of identity of two independent samples PI using all 11 STRs loci in ZW amounted to 3.118 x 10⁻¹⁰ and to 5.921 x 10⁻¹⁰ in ZS while the probability of identity related individuals PISibs was 1.331 x 10⁻⁴ and 1.749 x 10⁻⁴ in ZW and ZS. The power of exclusion for loci combinations when both parents are known, when only one of the parent is known and for two putative parents P1, P2 and P3 were in ZW versus ZS 0.99903 v. 0.99887, 0.97998 v. 0.97654 and 0.99999 v. 0.99998, respectively. Identified estimates of stated parameters illustrate suitability of tetrameric STRs for practical application in the management of genetic resources, verification of parentage and traceability in ZW and ZS. Based on the results, we recommend the panel of tetrameric STRs loci as suitable for parentage, traceability and differentiation of subpopulations in genetic pig resources of similar history. Hₑ.
Wydawca
-
Rocznik
Tom
35
Numer
3
Opis fizyczny
p.253-263,fig.,ref.
Twórcy
autor
  • Department of Morphology, Physiology and Animal Genetics, Mendel University in Brno, Zemedelska 1, 613 00 Brno, Czech Republic
  • Institute of Animal Breeding, Wroclaw University of Environmental and Life Sciences, Chelmonskiego 38c, 51-630 Wroclaw, Poland
  • Faculty of Animal Breeding and Biology, Poznan University of Life Sciences, Wojska Polskiego 28, 60-637 Poznan, Poland
autor
  • Department of Mathematics and Statistics, Msaryk University, Kotlarska 2, 611 37 Brno, Czech Republic
autor
  • Department of Morphology, Physiology and Animal Genetics, Mendel University in Brno, Zemedelska 1, 613 00 Brno, Czech Republic
Bibliografia
  • 1. ALVES E., FERNÁNDEZ A., BARRAGÁN C., OVILO C., RODRÍGUEZ C., SILIÓ L., 2006 - Inference of hidden population substructure of the Iberian pig breed using multilocus microsatellite data. Spanish Journal of Agricultural Research 4 (1), 37 - 46.
  • 2. ANONYM 2007 - Manual. Animaltype Pig kit, [Online] Available at: http://www.biotype.de//en/products/veterinary-medicine/animaltype.html. Accessed: December 2007.
  • 3. BERTHOULY-SALAZAR C., THÉVON S., NHU VAN T., NGUYEN B. T., PHAM L. D., VU CHU C., MAILLARD J. CH., 2012 - Uncontrolled admixture and loss of genetic diversity in a local Vietnamese pig breed. EcoL Evol. 2(5), 962 - 975.
  • 4. FABUEL E., BARRAGAN C., SILIO L., RODRIGUEZ M. C., TORO M. A., 2004 - Analysis of genetic diversity and conservation priorities in Iberian pigs based on microsatellite markers. Heredity 93, 104 - 113.
  • 5. GUASTELLA A. M., CRISCIONE A., MARLETTA D., ZUCCARO A., CHIES L., BORDANO S., 2010 - Molecular characterization and genetic structure of the Nero Siciliano pig breed. Genet. Mol. Biol. 33 (4), 650 - 656.
  • 6. HERRERO-MEDRANO J. M., MEGENS H. J., CROOIJMANS R. P., ABELLANEDA J. M., RAMIS G., 2012 - Farm-by-farm analysis of microsatellite, mtDNA and SNP genotype data reveals inbreeding and crossbreeding as threats to the survival of a native Spanish pig breed. Animal Genet., 44(3), 259 - 266.
  • 7. HERRERO-MEDRANO J. M., MEGENS H. J., CROOIJMANS R. P., ABELLANEDA J. M., RAMIS G. (2013): Farm-by-farm analysis of microsatellite, mtDNA and SNP genotype data reveals inbreeding and crossbreeding as threats to the survival of a native Spanish breed. Animal Genetics 44, 259 - 266.
  • 8. KURYŁ J., JANIK A., KAMYCZEK M., BUCZYŃSKI J. T., - 1997 - Genetic structure of Zlotnicka White and Zlotnicka Spotted pig as defined on the basis of the polymorphism of blood groups, serum proteins and lipoprotein allotype - a review. Animal Science Papers and Reports 15(3), 163 - 171.
  • 9. LI Y. H., CHU, H. P., JIANG Y. N., LIN C. Y., LI S.H., LI K. T., WENG G. J., CHENG C. C., LU D. J., JU Y. T., 2014 - Empirical Selection of Informative Microsatellite Markers within Co-ancestry Pig Populations Is Required for Improving the Individual Assignment Efficiency. Asian-Australas Journal of Animal Science 27(5), 616 - 627.
  • 10. MARTÍNEZ A. M., DELGADO J. V., RODERO A., VEGA-PLA J. L., 2000 - Genetic structure of the Iberian pig breed using microsatellites. Animal Genetics 31(5), 295 - 301.
  • 11. MARTÍNEZ A. M., LANDI V. P., MARTÍNEZ M. D., POTO A. R., PEINADO B. R., PONS A., BARBA C. C., CARRIL GONZÁLEZ-BARROS J. A., MÉNDEZ TUR Y., VEGA-PLA J. L., DELGADO J. V., 2012 - Genetic characterization of autochthonous pig breeds from Spain with microsatellite markers. In: De Pedro E. J. (ed.), Cabezas A. B. (ed.). 7th International Symposium on the Mediterranean Pig. Zaragoza: CIHEAM, 2012. p. 31 - 37. (Options Méditerranéennes: Série A. Séminaires Méditerranéens; n. 101). 7. International Symposium on the Mediterranean Pig, 2010/10/14-16, Córdoba (Spain).
  • 12. PARK S. D. E., 2001 - Trypanotolerance in West African Cattle and the Population Genetic Effects of Selection. [ Ph. D. thesis] University of Dublin.
  • 13. PEAKALL R., SMOUSE P. E., 2012 - Genalex 6.5: genetic analysis in Excel. Population genetic software for teaching and research-an update. Bioinformatics 28, 2537 - 2539.
  • 14. PRITCHARD JK, STEPHENS M., AND DONNELLY R., 2000 - Inference of population structure using multilocus genotype data. Genetics 155: 945 - 959.
  • 15. SZMATOLA T., ROPKA-MOLIK K., TYRA M., PIÓRKOWSKA K., ŻUKOWSKI K., OCZKOWICZ M., BLICHARSKI T., 2016 - The genetic structure of five pig breeds maintained in Poland. Annals of Animals Science 16, 4, 1019 - 1026.
  • 16. SZULC K., BUCZYŃSKI J. T., 2012 - Stare europejskie rasy świń. Poznań 2012, 124 - 127. ISBN 978-83-929756-5-6.
  • 17. TORO M., BARRAGÁN C., ÓVILO C., RODRIGANÉZ J., RODRIGUEZ C., SILIÓ L., 2002 - Estimation of coancestry in Iberian pigs using molecular markers. Conservation Genetics 3(3), 309 - 320.
  • 18. VRTKOVÁ I., 2015 - Genetic admixture analysis in Přeštice Black-Pied pigs. Arch. Anim. Breed., 58, 115-121.
  • 19. VRTKOVÁ I., VRTEK Š., FALKOVÁ L., 2016 - Efficiency of Tetrameric Short Tandem Repaets in Prestice Black Pied Pig for Traceability and Parentity Testing. Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis 64, 2, 557 - 565.
  • 20. WILKINSON S., HALEY C., ALDERSON L., WIENER R, 2011 - An empirical assessment of individual-based population genetic statistical techniques: application to British pig breeds. Heredity, 106 (2), 261 - 269.
Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
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