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2011 | 10 | 3 |
Tytuł artykułu

Histological and ultrastructural changes of hepatocytes in red-eared turtles (Trachemys scripta elegans) during hibernation and after arousal

Treść / Zawartość
Warianty tytułu
PL
Histologiczne i ultrastrukturalne zmiany w wątrobie u żółwia czerwonolicego (Trachemys scripta elegans) w czasie i po hibernacji
Języki publikacji
EN
Abstrakty
EN
This research was conducted on 22 red-eared sliders during hibernation and after arousal. The material came from Poznan Zoo (Poland). Species affiliation was established on the basis of the Turtles of the World key. The examined sliders were of a similar age. The histological, histochemical and ultrastructural studies were conducted during hibernation and after arousal. Additionally analysis of liver elemental composition (C, O2, Na, Ca, Al., P, Mg and S) was performed. The results showed morphological and ultrastructural liver changes, related to hibernation process concerning shape and size of hepatocytes as well as morphology and location of cell organelles. The conducted elemental analyses revealed during hibernation the reduction of the phosphorus levels up to70-80% while after arousal there was distinct increase of oxygen, sodium and aluminum concentration noticed.
PL
Badania wątroby przeprowadzono na 22 osobnikach płci męskiej żół­wia czerwonolicego w czasie hibernacji i po hibernacji. Materiał ten pochodził z Ogro­du Zoologicznego w Poznaniu. Gatunek żółwia określono na podstawie klucz Turtles of the Word [Ernst, Barbour 1989]. Wszystkie badane osobniki były w jednym wieku (8 lat). Przeprowadzono badania histologiczne, histochemiczne i ultrastrukturalne wątro­by. Wykonano również analizę chemiczną składu pierwiastkowego wątroby (C, O2, Na, Ca, Al., P, Mg i S). Badania morfologiczne i ultrastrukturalne wątroby wykazały zmiany w kształcie i wielkości hepatocytów w czasie hibernacji, jak również stwierdzono zmiany w budowie i rozmieszczeniu organelli komórkowych. Przeprowadzona analiza chemicz­na składu pierwiastkowego wątroby podczas hibernacji wykazała spadek poziomu fosforu o 70-80%, podczas gdy zaobserwowano wzrost koncentracji tlenu, sodu i aluminium.
Słowa kluczowe
Wydawca
-
Rocznik
Tom
10
Numer
3
Opis fizyczny
p.19-30,fig.,ref.
Twórcy
autor
  • Electron Microscope Laboratory, Wroclaw University of Environmental and Life Sciences, Kozuchowska 5b, 51-631 Wroclaw, Poland
  • Electron Microscope Laboratory, Wroclaw University of Environmental and Life Sciences, Kozuchowska 5b, 51-631 Wroclaw, Poland
autor
  • Electron Microscope Laboratory, Wroclaw University of Environmental and Life Sciences, Kozuchowska 5b, 51-631 Wroclaw, Poland
Bibliografia
  • Bami S., Vaccarone R., Bertone V., Fraschini A., Bernini F., and Fenoglio C., 2002. Mechanisms of changes to the liver pigmentary component during the annual cycle (activity and hibernation) of Rana esculenta L.J. Anat., (200), 185-194.
  • Churchill T.A., Cheetham K.M., Simpkin S., Green C.J., Wang L.C., Fuller B.J., 1994. Liver metabolism in cold hypoxia: a comparison of energy metabolism and glycolysis in cold-sensitive and cold-resistant mammals. J. Comp. Physiol., (164), 396-404.
  • Claussen D.L., Townsley M.D., Bausch R.G., 1990. Supercooling and freeze tolerance in the European wall lizard, Podarcis muralis, with a revisional history of the discovery of freeze tolerance in vertebrates. J. Comp. Physiol., (160), 137-143.
  • Constanzo J.P., Claussen D.L., 1990. Natural freeze tolerance in the terrestrail turtle, Terrapene Carolina. J. Exp. Zool., (254), 228-232.
  • Da Silva R.S.M., Migliorini R.H., 1990. Effects of starvations and refeeding on energy - linked metabolic processes in the turtle (Phrynops hilarii). Comp. Biochem. Physiol., (96), 415-419.
  • Ernst C.H., Barbour R.W., 1989. Turtles of the World. Copyrighted Material.
  • Fonda M.L., Herber G.H., Cuddihee R.W., 1983. Biochemical and morphometric studies of heart, liver and skeletal muscle from hibernating, arousing and aroused big brown bat, Eptesicus fus- cus. Comp. Biochem. Physiol., (76), 13-19.
  • French A.R., 1985. Allometries of the duration of torpid and eutermic intervals during mammalian hibernations test of the theory of metabolic control of the timing of changes in body tempera­ture. J. Comp. Physiol. (156), 13-19.
  • French A.R., 1988. The patterns of mammalian hibernation. Am. Sci., (76), 569-575.
  • Gans C., Gaunt A.S., 1998. Biology of Reptilia. Visceral organs. Society for the Study of Amphibians and Reptiles., (19), 485-531.
  • Girons S., Duguy R., 1992. Evolution de las masse corporelle et de la masse relative des corps gras, des ovaries et des oeufs au cours des cycles reproducteurs chez Vipera apis. Amphibia-Reptilia, (13), 351-364.
  • Hacking M.A., Budd J., Hodson K., 1987. The ultrastructure of the liver of the rainbow trout: nor­mal structure and modifications after chronic administration of polychlorinated biphenyl. Can. J. Zool. (56), 477-491.
  • Hasting M.H., Ebling F.J.P., 2006. Hibernation proteins: preparing for life in the freezer. Cell., (125), 21-23.
  • Hemmings S.J., Storey K.B., 2000. Hepatic changes in the freeze-tolerant turtle Chrysemys picta marginata in response to freezing and thawing. Cell. Biochem. Funct., (18), 175-86.
  • Malatesta M., Battistelli S., Rocchi M.B., Zancanaro C., Fakan S., Gazzanelli G., 2001. Fine structural modifications of liver, pancreas and brown adipose tissue mitochondria from hibernating, arousing and euthermic dormice. Cell. Biol. Int., (25), 131-138.
  • Mehrani H., Storey K.B., 1995. Enzymatic control of glycogenolysis during anoxic submergence in the freshwater turtle Trachemys scripta. Int. J. Biochem. Cell. Biol., (27), 821-830.
  • Miscalencu D., Iordachel M., Maila F., Mihaescu G., Untu C., 1978. Ultractructure of the liver cells in Salamandra salamandra L. in the end of winter. Acta Anat., (101), 10-18.
  • Rund C.R., Christiansen J.L., Johnson J.C., 1998. In vitro culture of melanomacrophages from the spleen and liver of turtles: comments on melanomacrophage morphology. Pigment. Cell. Res., (11), 114-119.
  • Storey K.B., Storey J.M., 1990. Metabolic rate depression and biochemical adaptation in anaerobiosis, hibernation and estivation. Q Rev. Biol., (65), 145-74.
  • Wang L.C.H., 1987. Mammalian hibernation, [in:] Grout B.W.W., Morris G.J. (eds) The effects of low temperature on biological systems. Arnold. London.
  • Warren D.E., Reese S.A., Jackson D.C, 2006. Tissue glycogen and extracellular buffering limit the survival of red-eared slider turtles during anoxic submergence at 3 degrees C. Physiol. Biochem. Zool., (79), 736-44.
  • Zimny M.L., Moreland J.E, 1968. Mitochondrial populations and succinic dehydrogenase in the heart of hibernator. Can. J. Physiol. Pharmacol., (46), 911-913.
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Bibliografia
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