The aim of the study was to determine the level of air bacteria and fungi in the horse stable and the emission of microorganisms to the external environment during different seasons of the year. The predominating fungi in the stable and outside were also identified. These results demonstrate that both in the stables and outdoors, the number of microorganisms was the highest in the summer. This tendency was especially noticeable in the case of the concentrations of fungi outside the building, when the average value was significantly higher (P<0.01) than in other seasons. No statistically significant differences in the concentrations of airborne microorganisms at different distances from the stable were observed. Penicillium sp., Fusarium sp., and yeast predominated inside and outside the stable.