Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2015 | 59 | 1 |

Tytuł artykułu

Results of serological and molecular analysis of African and classical swine fever in the population of wild boars in Ukraine

Warianty tytułu

Języki publikacji



The results of the study of lymphoid organs and sera of wild boars for the presence of DNA of African swine fever (ASF) virus and RNA of classical swine fever (CSF) virus are presented, as well as the results of a serological examination for the presence of ASF and CSF virus antibodies. The study was conducted in Ukraine between 2008 and 2013. Biological samples were obtained from wild boars shot during the hunting season, and were examined by real-time PCR and ELISA. In total, 5759 sera were tested for CSF virus antibodies and 4856 for ASF virus antibodies by ELISA. Samples of lymphoid organs totalling 1129 were examined by PCR for the detection of CSF virus RNA and 8102 such samples were examined for the detection of ASF virus DNA. CSF virus antibodies were detected in 6.56% of wild boar sera. RNA of CSF virus was also identified in 1 out of 1129 samples tested. ASF virus antibodies or DNA in lymphoid organ samples were not detected.

Słowa kluczowe








Opis fizyczny



  • Institute of Veterinary Medicine, National Academy of Agrarian Sciences of Ukraine, 03151, Kiev, Ukraine
  • Institute of Veterinary Medicine, National Academy of Agrarian Sciences of Ukraine, 03151, Kiev, Ukraine
  • Institute of Veterinary Medicine, National Academy of Agrarian Sciences of Ukraine, 03151, Kiev, Ukraine
  • Institute of Veterinary Medicine, National Academy of Agrarian Sciences of Ukraine, 03151, Kiev, Ukraine


  • 1. Arias M., Sanchez-Vizcaino J.M.: African swine fever. In: Trends in Emerging Viral Infections of Swine, edited by Morilla A., Yoon K.J., Zimmerman J.J., Iowa State Press, Ames, 2002, pp. 119-124.
  • 2. Bakulov I.A., Makarov V.V.: Problems of the modern evolution of African swine fever. J Agricult Sci 1990, 3, 46-55.
  • 3. Balyshev V.M.: The study of mixed infection of African and classical swine fever in experimental conditions. In: Materials of the international scientific-practical conference "Biological and ecological problems of infectious diseases of wild animals and their role in the pathology of agricultural animals and people", Pokrov, 2002, pp.98-100.
  • 4. Bech-Nielsen S., Bonilla Q.P., Sanchez-Vizcaino J.M.: Benefit-cost analysis of the current African swine fever eradication program in Spain and of an accelerated program. Prev Vet Med 1993, 17, 235-249, 10.1016/0167-5877(93) 90032-0.
  • 5. EFSA. Scientific opinion on the role of tick vectors in the epidemiology of Crimean-Congo hemorrhagic fever and African swine fever in Eurasia EFSA J 2010, 8, 170.
  • 6. FAO. African swine fever in the Caucasus. EMPRES Watch, 2008.
  • 7. FAO. African swine fever spread in the Russian Federation and the risk for the region. EMPRES Watch, 2009.
  • 8. Gallardo C., Okoth E., Pelayo V., Anchuelo R., Martin E., Simon A., Llorente A., Nieto R., Soler A., Martin R., Arias M., Bishop R.P.: African swine fever viruses with two different genotypes, both of which occur in domestic pigs, are associated with ticks and adult warthogs, respectively, at a single geographical site. J Gen Virol 2011, 92, 432-444.
  • 9. Gallardo C., Reis A.L., Kalema-Zikusoka G., Malta J., Soler A., Blanco E., Parkhouse R.M., Leitao A.: Recombinant antigen targets for serodiagnosis of African swine fever. Clin Vaccine Immunol 2009, 16, 1012-1020.
  • 10. Genkina R.: Scientists and managers of veterinary service discussed ways to eliminate ASF in Russia. Vet Life 2010, 20, 4-5.
  • 11. Hermanns W., Trautwein G., Meyer H., Liess B.: Experimental transmission of hog cholera virus in pigs. Zbl Vet Med B. 1981, 28, 669-683.
  • 12. Nychyk S.A., Sutyuk M., Nevolko O.M., Marushchak L.V.: African swine fever: peculiarities of manifestation in Ukraine. In: Programme & Abstract, 14th International Conference of the Association of Institutions for Tropical Veterinary Medicine, Johannesburg, South Africa, 2013, p. 49.
  • 13. Penrith M.-L., Guberti V., Depner K., Lubroth J.: Preparation of African swine fever contingency plans. Yerevan, Food and Agriculture Organization of the United Nations, 2011, p. 77.
  • 14. Perez-Filgueira D.M., Gonzalez-Camacho F., Gallardo C., Resino-Talavan P., Blanco E., Gomez-Casado E., Alonso C., Escribano J.M.: Optimization and validation of recombinant serological tests for African swine fever diagnosis based on detection of the p30 protein produced in Trichoplusiani larvae. J Clin Microbiol 2006, 44, 3114-3121.
  • 15. Pryskoka V.A., Sobko Y.A., Aranci S.V.: Classical swine fever (problems and prospects), Kiev, 2000, p. 180.
  • 16. Shykov A.T., Hryshok L.P., Astakhova O.V.: Strategy and tactics of control of classical swine fever in Ukraine. In: International scientific and practical conference. Actual problems of pathology of agricultural animals, Voronez, 2000, pp. 227-230.
  • 17. Shykov A.T., Hryshok L.P.: Epizootological zoning - a fundamental principle of prevention and control of classical swine fever in Ukraine. In: Ecological aspects of epizootiology and pathology of animals, Voronezh, 1999, pp. 43-46.

Typ dokumentu



Identyfikator YADDA

JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.