Evaluation of serum cytokine levels in recurrent airway obstruction

• Autorzy: Niedzwiedz A. , Borowicz H. , Kubiak K. , Nicpon J. , Skrzypczak P. , Jaworski Z. , Cegielski M. , Nicpon J.
• Języki publikacji: EN

Abstrakty

EN

Recurrent airway obstruction (RAO) represents a serious health problem and is traditionally classified as an allergic disease, where contact with an antigen can induce clinical airway inflammation, bronchial hyper-responsiveness and reversible airway obstruction. Previous studies have demonstrated the presence of the Th2 response in the lungs of human patients with asthma and horses with heaves. These cells are involved in the production of cytokines which regulate the synthesis of immunoglobulins. 40 horses were evaluated: 30 horses with RAO and 10 healthy animals. The expression levels of interferon-alpha 1 (IFN-α1), interferon-gamma (IFN-γ), interleukin-1β, (IL-1β), IL-2, IL-4, IL-13 and tumor necrosis factor alpha (TNF-α) were measured in the serum obtained from control and RAO-susceptible horses during crisis. In all the patients, serum cytokine levels were detected. Serum median IL-13 and IFN-γ levels were significantly higher in RAO-affected horses than in the healthy group (p < 0.001). The serum median IFN-α1, IL-1β, IL-2, IL-4, and TNF-α levels were similar in both groups. These results indicate a low variability of the levels of cytokines and a high frequency of their detection in serum samples from horses with RAO. Immune mechanisms involved in equine RAO are more complex than those defined by a simple Th1/Th2 dichotomy.

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 2016
• Tom: 19
• Numer: 4
• Opis fizyczny: p.785-791,fig.,ref.

Twórcy

autor

Niedzwiedz A.

• Department of Internal Diseases with Clinic for Horses, Dogs and Cats, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences. Pl. Grunwaldzki 47, 50-366 Wroclaw, Poland

autor

Borowicz H.

• Department of Internal Diseases with Clinic for Horses, Dogs and Cats, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences. Pl. Grunwaldzki 47, 50-366 Wroclaw, Poland

autor

Kubiak K.

• Department of Internal Diseases with Clinic for Horses, Dogs and Cats, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences. Pl. Grunwaldzki 47, 50-366 Wroclaw, Poland

autor

Nicpon J.

• Department of Surgery, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences Pl. Grunwaldzki 47, 50-366 Wroclaw, Poland

autor

Skrzypczak P.

• Department of Surgery, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences Pl. Grunwaldzki 47, 50-366 Wroclaw, Poland

autor

Jaworski Z.

• Department of Horse Breeding and Riding, University of Warmia and Mazury, Prawochenskiego 2, 10-720 Olsztyn, Poland

autor

Cegielski M.

• Department of Histology and Embryology, Medical University of Wroclaw, Chalubinskiego 6a, 50-368 Wroclaw, Poland

autor

Nicpon J.

• Department of Internal Diseases with Clinic for Horses, Dogs and Cats, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences. Pl. Grunwaldzki 47, 50-366 Wroclaw, Poland

Bibliografia

• Ainsworth DM, Grünig G, Matychak MB, Young J, Wagner B, Erb HN, Antczak DF (2003) Recurrent airway obstruction (RAO) in horses is characterized by IFN-gamma and IL-8 production in bronchoalveolar lavage cells. Vet Immunol Immunopathol 96: 83-91.
• Cian F, Monti P, Durham A (2015) Cytology of the lower respiratory tract in horses: An updated review. Equine Vet Educ 27: 544-553.
• Cordeau ME, Joubert P, Dewachi O, Hamid Q, Lavoie JP (2004) IL-4, IL-5 and IFN-gamma mRNA expression in pulmonary lymphocytes in equine heaves. Vet Immunol Immunopathol 97: 87-96.
• Couëtil LL, Cardwell JM, Gerber V, Lavoie JP, Léguillette R, Richard EA (2016) Inflammatory Airway Disease of Horses-Revised Consensus Statement. J Vet Intern Med 30: 503-515.
• Fernandez NJ, Hecker KG, Gilroy CV, Warren AL, Leguillette R (2013) Reliability of 400-cell and 5-field leukocyte differential counts for equine bronchoalveolar lavage fluid. Vet Clin Pathol 42: 92-98.
• Franchini M, Gilli U, Akens MK, Fellenberg RV, Bracher V (1998) The role of neutrophil chemotactic cytokines in the pathogenesis of equine chronic obstructive pulmonary disease (COPD). Vet Immunol Immunopathol 66: 53-65.
• Gerber V, Tessier C, Marti E (2015) Genetics of upper and lower airway diseases in the horse. Equine Vet J 47: 390-397.
• Giguére S, Viel L, Lee E, MacKay RJ, Hernandez J, Franchini M (2002) Cytokine induction in pulmonary airways of horses with heaves and effect of therapy with inhaled fluticasone propionate. Vet Immunol Immunopathol 85: 147-158.
• Horohov DW, Beadle RE, Mouch S, Pourciau SS (2005) Temporal regulation of cytokine mRNA expression in equine recurrent airway obstruction. Vet Immunol Immunopathol 108: 237-245.
• Kleiber C, McGorum BC, Horohov DW, Pirie RS, Zurbriggen A, Straub R (2005) Cytokine profiles of peripheral blood and airway CD4 and CD8 T lymphocytes in horses with recurrent airway obstruction. Vet Immunol Immunopathol 104: 91-97.
• Laan TT, Bull S, PirieR, Fink-Gremmels J (2006) The role of alveolar macrophages in the pathogenesis of recurrent airway obstruction in horses. J Vet Intern Med 20: 167-174.
• Lavoie JP, HorohovDW (2001) Cytokine immunoregulatory elements in RAO. Equine Immunology. R&W Publications, Newmarket, pp. 73-82.
• Leguillette R (2003) Recurrent airway obstruction-heaves. Vet Clin North Am Equine Pract 19: 63-86.
• Moore WC, Hasday JD, Meltzer SS, Wisnewski PL, White B, Bleecker ER (2001) Subjects with mild and moderate asthma respond to segmental allergen challenge with similar, reproducible, allergen-specific inflammation. J Allergy Clin Immunol 108: 908-914.
• Niedzwiedz A, Jaworski Z (2014) Oxidant-antioxidant status in the blood of horses with symptomatic recurrent airway obstruction (RAO). J Vet Intern Med 28: 1845-1852.
• Niedzwiedz A, Jaworski Z, Tykalowski B, Smialek M (2014) Neutrophil and macrophage apoptosis in bronchoalveolar lavage fluid from healthy horses and horses with recurrent airway obstruction (RAO). BMC Vet Res 10: 29
• Niedźwiedź A (2014) Equine recurrent airway obstruction. Mac Vet Rev 37: 115-120.
• Pacholewska A, Jagannathan V, Drögemüller M, Klukowska-Rötzler J, Lanz S, Hamza E, Dermitzakis ET, Marti E, Leeb T, Gerber V (2015) Impaired cell cycle regulation in a natural equine model of asthma. PloS One 10: e0136103.
• Padoan E, Ferraresso S, Pegolo S, Castagnaro M, Barnini C, Bargelloni L (2013) Real time RT-PCR analysis of inflammatory mediator expression in recurrent airway obstruction-affected horses. Vet Immunol Immunopathol 156: 190-199.
• Pirie RS, Dixon, PM, Collie DD, McGorum BC (2001) Pulmonary and systemic effects of inhaled endotoxin in control and heaves horses. Equine Vet J 33: 311-318.
• Stopyra A, Sobiech P, Wacławska-Matyjasik A (2012) Acid-base indicators in the venous and arterial blood of horses affected by recurrent airway obstruction (RAO). Pol J Vet Sci 15: 463-467.
• ten Hacken NH, Oosterhoff Y, Kauffman HF, Guevarra L, Satoh T, Tollerud DJ, Postma DS (1998) Elevated serum interferon-gamma in atopic asthma correlates with increased airways responsiveness and circadian peak expiratory flow variation. Eur Respir J 11: 312-316.
• Tilley P, Sales Luis JP, Branco Ferreira M (2012) Correlation and discriminant analysis between clinical, endoscopic, thoracic X-ray and bronchoalveolar lavage fluid cytology scores, for staging horses with recurrent airway obstruction (RAO). Res Vet Sci 93: 1006-1014.
• Tsoumakidou M, Tzanakis N, Kyriakou D, Chrysofakis G, Siafakas NM (2004) Inflammatory cell profiles and T-lymphocyte subsets in chronic obstructive pulmonary disease and severe persistent asthma. Clin Exp Allergy 34: 234-240.
• Wills-Karp M, Luyimbazi J, Xu X, Schofield B, Neben TY, Karp CL, Donaldson DD (1998) Interleukin-13: central mediator of allergic asthma. Science 282: 2258-2261.
• Wysocka B, Kluciński W (2015) Cytological evaluation of tracheal aspirate and broncho-alveolar lavage fluid in comparison to endoscopic assessment of lower airways in horses with recurrent airways obstruction or inflammatory airway disease. Pol J Vet Sci 18: 587-597.

Identyfikatory

DOI

10.1515/pjvs-2016-0099