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2010 | 32 | 5 |

Tytuł artykułu

Shoot regeneration from cotyledonary leaf explants of Jatropha curcas: a biodiesel plant

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Języki publikacji



A simple, high frequency, and reproducible method for plant regeneration through direct organogenesis from cotyledonary leaf explants of Jatropha curcas was developed using Murashige and Skoog (MS) medium supplemented with different concentrations of thidiazuron (TDZ) or 6-benzyl aminopurine (BAP). Medium containing TDZ has greater influence on regeneration as compared to BAP. The induced shoot buds were transferred to MS medium containing 10 µM kinetin (Kn), 4.5 µM BAP, and 5.5 µM α-naphthaleneacetic acid (NAA) for shoot proliferation. The proliferated shoots could be elongated on MS medium supplemented with different concentrations and combinations of BAP, indole-3-acetic acid (IAA), NAA, and indole-3-butyric acid (IBA). MS medium with 2.25 µM BAP and 8.5 µM IAA was found to be the best combination for shoot elongation. However, significant differences in plant regeneration and shoot elongation were observed among the genotypes studied. Rooting was achieved when the basal cut end of elongated shoots were dipped in half strength MS liquid medium containing different concentrations and combinations of IBA, IAA, and NAA for 4 days, followed by transfer to growth regulators free half strength MS medium supplemented 0.25 mg l⁻¹ activated charcoal. Elongated shoot treated with 15 µM IBA, 5.7 µM IAA, and 11 µM NAA resulted in highest percent rooting. The rooted plants could be established in soil with more than 90% survival rate. The method developed may be useful in improvement of J. curcas through genetic modification.

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  • Department of Agricultural Biotechnology, Anand Agricultural University, Anand 388110, Gujarat, India
  • Discipline of Wasteland Research, Central Salt and Marine Chemicals Research Institute (Council of Scientific and Industrial Research, New Delhi), Bhavnagar 364021, Gujarat, India
  • Discipline of Wasteland Research, Central Salt and Marine Chemicals Research Institute (Council of Scientific and Industrial Research, New Delhi), Bhavnagar 364021, Gujarat, India
  • Discipline of Wasteland Research, Central Salt and Marine Chemicals Research Institute (Council of Scientific and Industrial Research, New Delhi), Bhavnagar 364021, Gujarat, India
  • Plant Stress Genomics and Technology Center, King Abdullah University of Science and Technology, Thuwal 23955-6900, Kingdom of Saudi Arabia


  • Azad MAK, Yokota S, Ohkubo T, Andoh Y, Yahara S, Yoshizawa N (2005) In vitro regeneration of the medicinal woody plant Phellodendron amurense Rupr. through excised leaves. Plant Cell Tiss Organ Cult 80:43–50
  • Blakesey D (1991) Uptake and metabolosm of 6-benzyladenine in shoot proliferation of Musa and Rhododendron. Plant Cell Tiss Organ Cult 25:69–74
  • Chitra DS, Padmaja G (2005) Shoot regeneration via direct organogenesis from in vitro derived leaves of mulberry using thidiazuron and 6-benzylaminopurine. Sci Hortic 106:593–602
  • Christopher T, Rajam MV (1996) Effect of genotype, explant and medium on in vitro regeneration of red pepper. Plant Cell Tiss Organ Cult 46:245–250
  • da Camara Machado A, Frick NS, Kremen R, Katinger H, da Camara Machado ML (1997) Biotechnological approaches to the improvement of Jatropha curcas. In: Proceedings of the international symposium on jatropha, Nicaragua
  • Deore AC, Johnson TS (2008) High-frequency plant regeneration from leaf-disc cultures of Jatropha curcas L.: an important biodiesel plant. Plant Biotech Rep 2:10–15
  • Feyissa T, Welander M, Negash L (2005) In vitro regeneration of Hagenia abyssinica (Bruce) J.F. Gmel. (Rosaceae) from leaf explants. Plant Cell Rep 24:392–400
  • Francis G, Edingger R, Becker K (2005) A concept for simultaneous wasteland reclamation, fuel production, and socio-economic development in degraded areas in India. Need, potential and perspectives of Jatropha plantations. Nat Res Forum 29:12–24
  • Ghosh A, Chaudhary DR, Reddy MP, Rao SN, Chikara J, Pandya JB, Patolia JS, Gandhi MR, Adimurthy S, Vaghela N, Mishra S, Rathod MR, Prakash AR, Shethia BD, Upadhyay SC, Balakrishna V, Prakash CR, Ghosh PK (2007) Prospects for Jatropha methyl ester (biodiesel) in India. Int J Environ Stud 64:659–674
  • Guda CD, Castello S, Savona M, Farina E (2003) Echinacea purpurea—in vivo and in vitro propagation and field evaluation of ornamental clones. Col Prot 32:101–107
  • Hare PD, Van Staden J (1994) Inhibitory effect of thidiazuron on the activity of cytokinin oxidase isolated from soybean callus. Plant Cell Physiol 35:1121–1125
  • Henry Y, Vain P, Buyser JD (1994) Genetic analysis of in vitro plant tissue culture responses and regeneration capacities. Euphytica 79:45–58
  • Huetteman CA, Preece JE (1993) Thidiazuron: a potent cytokinin for woody plant tissue culture. Plant Cell Tiss Organ Cul 33:105–119
  • Hyde CL, Phillips GC (1996) Silver nitrate promotes shoot development and plant regeneration of Chilepepper (Capsicum annuum L.) via organogenesis. In Vitro Cell Dev Biol Plant 32:72–80
  • Jha T, Mukherjee P, Datta MM (2007) Somatic embryogenesis in Jatropha curcas Linn., an important biofuel plant. Plant Biotech Rep 1:135–140
  • Kaminek M (1992) Progress in cytokinin research. Trends Biotech 10:159–162
  • Koroch A, Juliani HR, Kapteyn J, Simon JE (2002) In vitro regeneration of Echinacea purpurea from leaf explants. Plant Cell Tiss Organ Cult 69:79–83
  • Kumar N, Pamidimarri SDVN, Kaur M, Boricha G, Reddy MP (2008) Effects of NaCl on growth, ion accumulation, protein, proline contents, and antioxidant enzymes activity in callus cultures of Jatropha curcas. Biologia 63:378–382
  • Lemaux PG (2008) Genetically engineered plants and foods: a scientist’s analysis of the issues (Part I). Annu Rev Plant Biol 59:771–812
  • Lin HS, De Jeu MJ, Jacobsen E (1997) Direct shoot regeneration from excised leaf explants of in vitro grown seedlings of Alstroemeria L. Plant Cell Rep 16:770–774
  • Magioli C, Rocha APM, de Oliveira DE, Mansur E (1998) Efficient shoot organogenesis of eggplant (Solanum melongena L.) induced by thidiazuron. Plant Cell Rep 17:661–663
  • Martin KP (2003) Rapid axillary bud proliferation and ex vitro rooting of Eupatorium triplinerve. Biol Plant 47:589–591
  • Mondal TK, Bhattacharya A, Sood A, Ahuja PS (1998) Micropropagation of tea (Camellia sinensis (L.) O. Kuntze) using thidiazuron. Plant Growth Regul 26:57–61
  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–479
  • Nandagopal S, Ranjitha Kumari BD (2007) Effectiveness of auxin induced in vitro root culture in chicory. J Cen Eur Agri 8:73–79
  • Nielsen JM, Kirsten B, Hansen J (1993) Long-term effects of thidiazuron are intermediate between benzyladenine, kinetin or isopentenyladenine in Miscanthus sinensis. Plant Cell Tiss Organ Cult 35:173–179
  • Openshaw K (2000) A reviews of Jatropha curcas: an oil plant of unfulfilled promise. Biomass Bioeng 19:1–5
  • Ozaslan M, Can C, Aytekin T (2005) Effect of explant source on in vitro propagation of Paulownia tomentosa Steud. Biotech Biotech Equ 19:20–26
  • Pellegrineschi A (1997) In vitro plant regeneration via organogenesis of cowpea [Vigna unguiculata (L.) Walp.]. Plant Cell Rep 17:89–95
  • Preece JE, Imel MR (1991) Plant regeneration from leaf explants of Rhododendron ‘P.J.M. Hybrids’. Sci Hortic 48:159–170
  • Raghu AV, Geetha SP, Martin G, Balachandran I, Ravindran PN (2006) Direct organogenesis from leaf explants of Embelia ribes Burm.—a vulnerable medicinal plant. J For Res 11:57–60
  • Rajore S, Batra A (2007) An alternative source for regenerable organogenic callus induction in Jatropha curcas. Ind J Biotech 6:545–548
  • Reddy MP, Kumar N, Vijay Anand KG, Singh AH, Singh S (2008) Method for micropropagation of Jatropha curcas plants from leaf explants (Patent filed US and PCT, File No. 2537de2008).
  • Schween G, Schwenkel HG (2003) Effect of genotype on callus induction, shoot regeneration, and phenotypic stability of regenerated plants in greenhouse of Primula ssp. Plant Cell Tiss Organ Cult 72:53–61
  • Sharma KK, Bhatnagar-Mathur P, Thorpe TA (2005) Genetic transformation technology: status and problems. In Vitro Cell Dev Biol Plant 41:102–112
  • Singh A, Reddy MP, Patolia JS (2008) An improved protocol for micropropagation of elite genotypes of Simmondsia chinensis (Link) Schneider. Biol Plant 52:538–540
  • Sujatha M, Mukta N (1996) Morphogenesis and plant regeneration from tissue cultures of Jatropha curcas. Plant Cell Tissue Organ Cult 44:135–141
  • Sujatha M, Makkar HPS, Becker K (2005) Shoot bud proliferation from axillary nodes and leaf sections of non-toxic Jatropha curcas L. Plant Growth Regul 47:83–90
  • Venkataiah P, Subhash K (2003) Thidiazuron-induced adventitious shoot bud formation and plant regeneration in Capsicum annuum L. J Plant Biotech 5:245–250
  • Vuylasteker C, Dewaele S, Rambour S (1998) Auxin induced lateral root formation in chicory. Ann Bot 81:449–454
  • Wei Q, Wei-Da L, Liao Y, Shu-Lin P, Xu Y, Tang L, Fang C (2004) Plant regeneration from epicotyl explants of Jatropha curcas. J Plant Physiol Mol Biol 30:475–478

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