The polymorphism of bovine FIT gene and its associations with cattle (Bos taurus) growth traits

• Autorzy: Sun J , Zhang C. , Fang X. , Lei C. , Lan X. , Chen H.
• Języki publikacji: EN

Abstrakty

EN

Fat-inducing transcript (FIT) are endoplasmic reticulum-resident membrane proteins that induce lipid droplet accumulation. It plays a crucial role in the fundamental process of storing fat. In this study, applying the PCR-SSCP and DNA sequencing methods, polymorphism of the FIT gene were detected. A total of 708 individuals from four Chinese cattle breeds were examined. The results showed that only P5 locus had two SNPs, resulting in a synonymous mutation (NM_001103095: m.199G > T resulting In L124L) and a missense mutation (NM_001103095: m.434G > T resulting in V176L). The associations between polymorphic loci and selected growth traits of indigenous Nangyang cattle were analysed,and significant associations were found in body weight at the age of 12 months and mean daily live weight gain. The body weight at month 12 of life and mean daily live weight gain of individuals with genotype AA were by 3.75% and 4.88% higher than of those with genotype AB, respectively. Hence, it was suggested for the first time, that genotype AA could be regarded as molecular marker for superior body weight and daily live weight gain in Chinese Nanyang cattle.

Słowa kluczowe

EN

gene polymorphism; FIT gene; gene; association; cattle; Bos taurus; growth trait

• Wydawca: -
• Czasopismo: Animal Science Papers and Reports
• Rocznik: 2011
• Tom: 29
• Numer: 2
• Opis fizyczny: p.99-107,fig.,ref.

Twórcy

autor

Sun J

• Institute of Cellular and Molecular Biology, Xuzhou Normal University, Xuzhou, Jiangsu 221116, China

autor

Zhang C.

autor

Fang X.

autor

Lei C.

autor

Lan X.

autor

Chen H.

Bibliografia

• BELLER M., RIEDEL D., JANSCH L., DIETERICH G.., WEHLAND J., JACKLE H., KUHNLEIN R.P., 2006 – Characterization of the Drosophila lipid droplet subproteome. Molecular Cell Proteomics 5, 1082-1094.
• BOTSTEIN D., WHITE R.L., SKOLNICK M., DAVIS R.W., 1980 – Construction of a genetic linkage map in man using restriction fragment length polymorphisms. American Journal of Human Genetics 32, 314-331.
• BRASAEMLE D.L., DOLIOS G.., SHAPIRO L., WANG R., 2004 – Proteomic analysis of proteins associated with lipid droplets of basal and lipolytically stimulated 3T3-L1 adipocytes. Journal of Biological Chemistry 279, 46835-46842.
• CAPON F., ALLEN M.H., AMEEN M., BURDEN A.D., TILLMAN D., BARKERAND J.N.,TREMBATH R.C., 2004 – A synonymous SNP of the corneodesmosin gene leads to increased mRNA stability and demonstrates association with psoriasis across diverse ethnic groups. Human Molecular Genetics 13, 2361-2368.
• CHANG B.H., CHAN L., 2007 – Regulation of Triglyceride Metabolism III Emerging role of lipid droplet protein ADFP in health and disease. Am.J.Physiol Gastrointest. Liver Physiology 292,G1465-G1468.
• MARTIN S., PARTON R.G., 2006 – Lipid droplets: a unified view of a dynamic organelle. Nat.Rev.Mol.Cell Biol. 7, 373-378.
• LIU P., YING Y., ZHAO Y., MUNDY D.I., ZHU M., Anderson R.G., 2004 – Chinese hamster ovary K2 cell lipid droplets appear to be metabolic organelles involved in membrane traffic. Journal of Biological Chemistry 279, 3787-3792.
• KADEREIT B., KUMAR P., WEN-JUN W., MIRANDA D., SNAPP E.L., SEVERINA N.,TORREGROZA I., EVANS T., SILVER D.L., 2008 – Evolutionarily conserved gene family important for fat storage. Proceedings of the National Academy of Sciences of the USA 105, 94-99.
• KIMCHI-SARFATY C., OH J.M., KIM I.W., SAUNA Z.E., CALCAGNO A.M., AMBUDKAR S.V., GOTTESMAN M.M., 2007 – A “silent” polymorphism in the MDR1 gene changes substrate specificity. Science 315, 525- 528.
• MEAD R., CURNOW R.N., 1985 – Statistical Methods in Agriculture and Experimental Biology.Chapman and Hall, London, UK
• MULLENBACH R., LAGODA P.J., WELTER C., 1989 – An efficient salt chloroform extraction of DNA from blood and tissue. Trends in Genetics 5, 391.
• MULLNER H., DAUM G., 2004 – Dynamics of neutral lipid storage in yeast. Acta Biochimica Polonica 51, 323-347.
• NACKLEY A.G., SHABALINA S.A., TCHIVILEVA I.E., SATTERFIELD K.KORCHYNSKYI O., MAKAROV S.S., MAIXNER W., DIATCHENKO L., 2006 – Human catechol-Omethyltransferase haplotypes modulate protein expression by altering mRNA secondary structure.Science 314, 1930–1933.
• SPIEGELMAN B.M., FLIER J.S., 2001 – Obesity and the regulation of energy balance. Cell 104,531-543.
• VAIMAN D., MERCIER D., MOAZAMI-GOUDARZI K., EGGEN A., CIAMPOLINI R.,LEPINGLE A., VELMALA R., KAUKINEN J., VARVIO S.L., MARTIN P., LEVÉZIEL H.,GUÉRIN G., 1994 – A set of 99 cattle microsatellites characterization, synteny mapping and polymorphism. Mammalian Genome 5, 288-297.