Influence of chemically and biosynthesized silver nanoparticles on in vitro viability and infectivity of Trichinella spiralis muscle larvae

• Autorzy: Abd-Elrahman S.M. , Dyab A.K. , Mahmoud A.E.-S. , Alsharif F.M. , Mohamed S.M. , Abomughaid M.M. , Elossily N.A.
• Języki publikacji: EN

Abstrakty

EN

Trichinellosis is a serious worldwide parasitic zoonosis. The available therapy for the treatment of Trichinella spiralis is not satisfactory. Therefore, the recovery of effective treatment is required. This work aimed at evaluating of the in vitro effect of silver nanoparticles (AgNPs) on muscle larvae of Trichinella. The present study investigated the larvicidal properties of chemical and myrrh AgNPs on muscle larvae (ML) of T. spiralis. The used AgNPs were chemically prepared using NaBH4 as reducing agent and biosynthesized using methanolic myrrh extract. Characterization of synthesized AgNPs was monitored via UV-vis pectrophotometry, Fourier transform infrared spectroscopy and transmission electron microscopy (TEM) studies. The ML incubated with AgNPs at concentrations ranged from 1 μg/ml to 20 μg/ml. Chemical and biosynthesized AgNPs revealed marked larvicidal effect against ML of Trichinella. Additionally, this in vitro study showed degenerative changes affecting the cuticle of AgNPs treated ML. The effectiveness of AgNPs on the infectivity of Trichinella ML was also assessed. The results showed complete inhibition of the infectivity of ML exposed to sublethal doses of chemical and myrrh prepared AgNPs when used to infect animal models. This is the first report where myrrh synthesized AgNPs have been tested for their anthelminthic activity against Trichinella in an in vitro model.

• Wydawca: -
• Czasopismo: Annals of Parasitology
• Rocznik: 2021
• Tom: 67
• Numer: 4
• Opis fizyczny: p.591-602,fig.,ref.

Twórcy

autor

Abd-Elrahman S.M.

• Department of Parasitology, Faculty of Veterinary Medicine, Assiut University, Assiut, Egypt

autor

Dyab A.K.

• Department of Medical Parasitology, Faculty of Medicine 2, Assiut University, Assiut, Egypt

autor

Mahmoud A.E.-S.

• Department of Medical Parasitology, Faculty of Medicine 2, Assiut University, Assiut, Egypt

autor

Alsharif F.M.

• Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, Al Azhar University, Assiut, Egypt

autor

Mohamed S.M.

• Department of Pharmacognosy, Faculty of Pharmacy, Assiut University, Assiut, Egypt

autor

Abomughaid M.M.

• Department of Medical Laboratory Sciences, University of Bisha, Bisha, Saudia Arabia

autor

Elossily N.A.

• Department of Medical Parasitology, Faculty of Medicine 2, Assiut University, Assiut, Egypt

Identyfikatory

DOI

10.17420/ap6704.375