Concentration of serum amyloid A and ceruloplasmin activity in milk from cows with subclinical mastitis caused by different pathogens
Treść / Zawartość
The aim of the present study was to determine the concentration of serum amyloid A (SAA) and the activity of ceruloplasmin (Cp) in milk from cows with subclinical mastitis caused by different pathogens. Eighty-four milk samples from cows with subclinical mastitis and fourteen milk samples from healthy cows were examined. SAA concentration was determined using the commercial ELISA kit (Tridelta Development Ltd., Greystones, Wicklow, Ireland). Cp activity was assessed spectrophotometrically, using the Rice method. The results reveal that the concentration of SAA (with exception of CNS) and activity of Cp in cow milk can be regarded as markers of subclinical mastitis, irrespective of the microorganism inducing the disease. In conclusion, measurement of SAA and Cp in milk samples could be a useful method in diagnosing subclinical mastitis in cows, but the method should be adapted for field use.
- Akerstedt M, Persson Waller K, Sternesjö A (2007) Haptoglobin and serum amyloid A in relation to the somatic cell count in quarter, cow composite and bulk tank milk samples. J Dairy Res 74: 198-203.
- Busato A, Trachsel P, Schallibaum M, Blum JW (2000) Udder health and risk factors for subclinical mastitis in organic dairy farms in Switzerland. Prev Vet Med 44: 205-220.
- Cerveza PJ, Mehrbod F, Cotton SJ, Lomeli N, Linder MC, Fonda EG, Wickler SJ (2000) Milk ceruloplasmin and its expression by mammary gland and liver in pigs. Arch Biochem Biophys 373: 451-461.
- Chacornac JP, Barnouin J, Raboisson T (1986) Automated microanalysis of plasma ceruloplasmin for the measurement of oxidase activity in cattle and sheep. Reprod Nutr Dev 26: 417-427.
- Chassagne M, Barnouin J, Chacornac JP (1998) Biological predictors for early clinical mastitis occurrence in Holstein cows under field conditions in France. Prev Vet Med 35: 29-38.
- Conner JG, Eckersall PD, Doherty M, Douglas TA (1986) Acute phase response and mastitis in the cow. Res Vet Sci 41: 126-128.
- Costa EO, Ribeiro AR, Watanabe ET, Melville PA (1998) Infectious bovine mastitis caused by environmental organisms. Zentralbl Veterinarmed B 45: 65-71.
- Devriese LA, De Keyser H (1980) Prevalence of different species of coagulase-negative staphylococci on teats and in milk samples from dairy cows. J Dairy Res 47: 155-158.
- Donley SA, Ilagan BJ, Rim H, Linder MC (2002) Copper transport to mammary gland and milk during lactation in rats. Am J Physiol Endocrinol Metab 283: E667-E675.
- Eckersall PD, Young FJ, McComb C, Hogarth CJ, Safi S, Weber A, McDonald T, Nolan AM, Fitzpatrick JL (2001) Acute phase proteins in serum and milk from dairy cows with clinical mastitis. Vet Rec 148: 35-41.
- Eckersall PD, Young FJ, Nolan AM, Knight CH, McComb C, Waterson MM, Hogarth CJ, Scott EM, Fitzpatrick JL (2006) Acute phase proteins in bovine milk in an experimental model of Staphylococcus aureus subclinical mastitis. J Dairy Sci 89: 1488-1501.
- Hiss S, Mueller U, Neu-Zahren A, Sauerwein H (2007) Haptoglobin and lactate dehydrogenase measurements in milk for the identification of subclinically diseased udder quarters. Vet Med (Praha) 52: 245-252.
- Grönlund U, Hallen Sandgren C, Persson Waller K (2005) Haptoglobin and serum amyloid A in milk from dairy cows with chronic sub-clinical mastitis. Vet Res 36: 191-198.
- Grönlund U, Hulten C, Eckersall PD, Hogarth C, Persson Waller K (2003) Haptoglobin and serum amyloid A in milk and serum during acute and chronic experimentally induced Staphylococcus aureus mastitis. J Dairy Res 70: 379-386.
- Jaeger JL, Shimizu N, Gitlin JD (1991) Tissue-specific ceruloplasmin gene expression in the mammary gland. Biochem J 280: 671-677.
- Kehrer JP (1993) Free radicals as mediators of tissue injury and disease. Crit Rev Toxicol 23: 21-48.
- Kim IG, Park SY, Kim KC, Yum JJ (1998) Thiol-linked peroxidase activity of human ceruloplasmin. FEBS Lett 431: 473-475.
- Kostro K, Gliński Z, Wójcicka-Lorenowicz K, Krakowski K (2001) Acute-phase proteins as indicators of diseases in animals. Med Weter 57: 539-542.
- Kovac G, Popelkova M, Tkacikova L, Burdova O, Ihnat O (2007) Interrelationship between somatic cell countand acute phase proteins in serum and milk of dairy cows. Acta Vet Brno 76: 51-57.
- Krukowski H (2001) Mycotic mastitis in cows. Med Weter 57: 18-20.
- Larson MA, Weber A, Weber AT, McDonald TL (2005) Differential expression and secretion of bovine serum amyloid A3 (SAA 3) by mammary epithelial cells stimulated with prolactin or lipopolysaccharide. Vet Immunol Immunopathol 107: 255-264.
- Lehtolainen T, Rontved C, Pyorala S (2004) Serum amyloid A and TNFα in serum and milk during experimental endotoxin mastitis. Vet Res 35: 651-659.
- McDonald TL, Larson MA, Mack DR, Weber A (2001) Elevated extrahepatic expression and secretion of mammary-associated serum amyloid A 3 (M-SAA 3) into colostrum. Vet Immunol Immunopathol 83: 203-211.
- Murata H, Shimada N, Yoshioka M (2004) Current research on acute phase proteins in veterinary diagnosis: an overview. Vet J 168: 28-40.
- Nielsen BH, Jacobsen S, Andersen PH, Niewold TA, Heegaard PM (2004) Acute phase protein concentrations in serum and milk from healthy cows, cows with clinical mastitis and cows with extramammary inflammatory conditions. Vet Rec 154: 361-365.
- O’Mahony MC, Healy AM, Harte D, Walshe KG, Torgerson PR, Doherty ML (2006) Milk amyloid A: correlation with cellular indices of mammary inflammation in cows with normal and raised serum amyloid A. Res Vet Sci 80: 155-161.
- Pedersen LH, Aalbaek B, Rontved CM, Ingvartsen KL, Sorensen NS, Heegaard PM, Jensen HE (2003) Early pathogenesis and inflammatory response in experimental bovine mastitis due to Streptococcus uberis. J Comp Pathol 128: 156-164.
- Petersen HH, Nielsen JP, Heegaard PM (2004) Application of acute phase protein measurements in veterinary clinical chemistry. Vet Res 35: 163-187.
- Pyörälä S, Hovinen M, Simojoki H, Fitzpatrick J, Eckersall PD, Orro T (2011) Acute phase proteins in milk in naturally acquired bovine mastitis caused by different pathogens. Vet Rec 168: 535.
- Pyörälä S, Syvajarvi J (1987) Bovine acute mastitis. Part I. Clinical aspects and parameters of inflammation in mastitis caused by different pathogens. Zentralbl Veterinarmed B 34: 573-584.
- Reneau JK, Packard VS (1991) Monitoring mastitis, milk quality and economic losses in dairy fields. Dairy, Food and Environmental Sanitation 11: 4-11.
- Rice EV, Wagman E, Takenaka Y (1963) Ceruloplasmin assay in serum: standardization of ceruloplasmin activity in terms of international enzyme units. Stand Methods Clin Chem 4: 39-47.
- Riollet C, Rainard P, Poutrel B (2000) Differential induction of complement fragment C5a and inflammatory cytokines during intramammary infections with Escherichia coli and Staphylococcus aureus. Clin Diagn Lab Immunol 7: 161-167.
- Seegers H, Fourichon C, Beaudeau F (2003) Production effects related to mastitis and mastitis economics in dairy cattle herds. Vet Res 34: 475-491
- Sheldon IM, Noakes DE, Rycroft A, Dobson H (2001) Acute phase protein responses to uterine bacterial contamination in cattle after calving. Vet Rec 148: 172-175.
- Simojoki H, Orro T, Taponen S, Pyörälä S (2009) Host response in bovine mastitis experimentally induced with Staphylococcus chromogenes. Vet Microbiol 134: 95-99.
- Suojala L, Orro T, Jarvinen H, Saatsi J, Pyörälä S (2008) Acute phase response in two consecutive experimentally induced E. coli intramammary infections in dairy cows. Acta Vet Scand 50: 18.
- Sutra L, Poutrel B (1994) Virulence factors involved in the pathogenesis of bovine intramammary infections due to Staphylococcus aureus. J Med Microbiol 40: 79-89.
- Szczubiał M, Dąbrowski R, Kankofer M, Bochniarz M, Albera E (2008) Concentration of serum amyloid A and activity of ceruloplasmin in milk from cows with clinical and subclinical mastitis. Bull Vet Inst Pulawy 52: 391-395.
- Taponen S, Pyörälä S (2009) Coagulase-negative staphylococci as cause of bovine mastitis – not so different from Staphylococcus aureus? Vet Microbiol 134: 29-36.
- Weber A, Weber AT, McDonald TL, Larson MA (2006) Staphylococcus aureus lipotechoic acid induces differential expression of bovine serum amyloid A3 (SAA 3) by mammary epithelial cells: Implications for early diagnosis of mastitis. Vet Immunol Immunopathol 109: 79-83.
- Wenz JR, Fox LK, Muller FJ, Rinaldi M, Zeng R, Bannerman DD (2010) Factors associated with concentrations of select cytokine and acute phase proteins in dairy cows with naturally occuring clinical mastitis. J Dairy Sci 93: 2458-2470.
- Winter P, Fuchs K, Walshe K, Colditz IG (2003) Serum amyloid A in the serum and milk of ewes with mastitis induced experimentally with Staphylococcus epidermidis. Vet Rec 152: 558-562.