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The effect was studied of the M. expansa homogenate administered with Freund’s complete adjuvant and Propionibacterium granulosum - on the humoral response of lambs spontaneously infected with Moniezia sp. Statistically significant increase in precipitating, complement fixing and haemagglutinating antibodies was observed in lambs immunized with homogenate injected with Freund’s adjuvant. Limitation of prevalence of infection observed in immunized animals was not statistically significant.
Three species of oribatid mites: Scheloribates latipes, Pergalumna nervosa and Ceratozetes sp. were experimentally infected with Moniezia expansa eggs or oncospheres. The intermediate hosts were kept under constant laboratory conditions at 27°C and 80% relative humidity. Three species of oribatid mites became infected and completely developed cestode cysticercoids were found. The early part of life cycle of M. expansa was studied in S. latipes. The mites were examined on 20th, 24th and 29th day after cestode oncosphera invasion. A fully fonned cysticercoid of M. expansa was observed on 29th day after infection. The mean of intensity of infection was 1-5 cysticercoides per mite. The infected and living oribatid mites could be kept under laboratory conditions for 7 months. The cysticercoides which had been recovered from S. latipes after this time were able to infect sheep.
One month old male Wistar rats were infected with 4,000 T. spiralis muscle larvae and exposed to intraperitoneal challenge with a lyophilized cell wall extract of Propionibacterium granulosum. The animals were arranged in groups which included naive controls, single and concurrent treatment. The latter comprised several groups differing only in the interval between infection with T. spiralis and administration of the bacterial extract. The intensity of infection was assessed during the intestinal (5 DAI, 10 DAI) and muscle (42 DAI) phases of infection, fecundity indices were calculated and changes in the relative concentration of peripheral blood leucocytes, erythrocytes and haemoglobin levels were monitored. No changes in erythrocyte concentration were detected in groups treated with the immunomodulator and infected with T. spiralis, but increased leucocyte counts, haematocrit and haemoglobin levels depended on the time of treatment in relation to infection and day on which the parameters were monitored. All rats treated with the bacterial extract had lower adult worm counts but harboured worms with enhanced fecundity relative to the control group. The intensity of the muscle phase was lower only in those groups treated with the bacterial extract after nematode infection.
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